ENSG00000088325

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000300403 ENSG00000088325 HEK293_OSMI2_2hA HEK293_TMG_2hB TPX2 protein_coding protein_coding 89.51111 81.18731 97.18336 4.323158 2.000679 0.2594257 66.028211 46.879739 78.8732840 5.6781150 2.2483105 0.7504474 0.72910000 0.57490000 0.811333333 0.236433333 3.730993e-05 2.252078e-38 FALSE TRUE
MSTRG.20534.16 ENSG00000088325 HEK293_OSMI2_2hA HEK293_TMG_2hB TPX2 protein_coding   89.51111 81.18731 97.18336 4.323158 2.000679 0.2594257 2.643914 6.018160 1.7267879 1.5181258 0.3928195 -1.7952961 0.03140833 0.07586667 0.017633333 -0.058233333 1.112850e-02 2.252078e-38 FALSE TRUE
MSTRG.20534.20 ENSG00000088325 HEK293_OSMI2_2hA HEK293_TMG_2hB TPX2 protein_coding   89.51111 81.18731 97.18336 4.323158 2.000679 0.2594257 2.744623 12.427286 0.9217407 2.9931131 0.2106818 -3.7385993 0.03397917 0.15473333 0.009433333 -0.145300000 2.156133e-09 2.252078e-38 FALSE TRUE
MSTRG.20534.23 ENSG00000088325 HEK293_OSMI2_2hA HEK293_TMG_2hB TPX2 protein_coding   89.51111 81.18731 97.18336 4.323158 2.000679 0.2594257 2.845679 5.909704 0.0000000 0.7469906 0.0000000 -9.2093813 0.03534167 0.07296667 0.000000000 -0.072966667 2.252078e-38 2.252078e-38 FALSE TRUE
MSTRG.20534.9 ENSG00000088325 HEK293_OSMI2_2hA HEK293_TMG_2hB TPX2 protein_coding   89.51111 81.18731 97.18336 4.323158 2.000679 0.2594257 8.455275 7.041317 8.3256361 1.2532083 0.6186708 0.2413995 0.09180417 0.08700000 0.085833333 -0.001166667 1.000000e+00 2.252078e-38 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000088325 E001 0.5858876 0.0206272084 3.693202e-01 5.137451e-01 20 31739149 31739196 48 + 0.277 0.132 -1.321
ENSG00000088325 E002 0.5890081 0.0294886837 9.179538e-01 9.521451e-01 20 31739197 31739233 37 + 0.202 0.233 0.260
ENSG00000088325 E003 2.1389287 0.1754975914 3.202097e-01 4.633150e-01 20 31739234 31739265 32 + 0.598 0.381 -1.076
ENSG00000088325 E004 1.9906355 0.1495468551 1.856677e-01 3.083264e-01 20 31739266 31739266 1 + 0.598 0.315 -1.478
ENSG00000088325 E005 2.2830573 0.1456958393 1.102768e-01 2.059564e-01 20 31739267 31739270 4 + 0.658 0.315 -1.740
ENSG00000088325 E006 2.2830573 0.1456958393 1.102768e-01 2.059564e-01 20 31739271 31739271 1 + 0.658 0.315 -1.740
ENSG00000088325 E007 3.6334163 0.1174184629 6.225681e-02 1.304311e-01 20 31739272 31739276 5 + 0.821 0.442 -1.669
ENSG00000088325 E008 4.5201447 0.1162825534 3.755764e-02 8.650961e-02 20 31739277 31739279 3 + 0.908 0.493 -1.750
ENSG00000088325 E009 11.2009811 0.0058670093 1.241052e-07 1.238287e-06 20 31739280 31739288 9 + 1.288 0.722 -2.105
ENSG00000088325 E010 13.1939085 0.0055776897 2.560378e-09 3.519744e-08 20 31739289 31739289 1 + 1.361 0.751 -2.244
ENSG00000088325 E011 20.5811965 0.0056483691 3.437772e-08 3.826980e-07 20 31739290 31739290 1 + 1.507 1.055 -1.591
ENSG00000088325 E012 41.6541567 0.0123427023 3.329637e-08 3.716649e-07 20 31739291 31739301 11 + 1.791 1.377 -1.413
ENSG00000088325 E013 154.4621153 0.0100110760 1.337673e-06 1.081136e-05 20 31739302 31739347 46 + 2.299 2.047 -0.841
ENSG00000088325 E014 524.8172531 0.0042371294 3.647996e-10 5.855803e-09 20 31739348 31739621 274 + 2.805 2.615 -0.635
ENSG00000088325 E015 437.6740734 0.0001557240 4.911028e-32 9.503366e-30 20 31742541 31742647 107 + 2.722 2.543 -0.597
ENSG00000088325 E016 0.8062886 0.0210846447 2.154359e-01 3.449491e-01 20 31744551 31744566 16 + 0.340 0.133 -1.732
ENSG00000088325 E017 1.8552408 0.1932363085 2.925740e-01 4.335403e-01 20 31744567 31744592 26 + 0.529 0.324 -1.101
ENSG00000088325 E018 2.3712091 0.0573045302 8.010625e-01 8.732299e-01 20 31744593 31744598 6 + 0.529 0.500 -0.138
ENSG00000088325 E019 3.5451598 0.0278958909 7.378332e-01 8.278953e-01 20 31744599 31744675 77 + 0.659 0.627 -0.140
ENSG00000088325 E020 251.1392026 0.0013851450 4.602676e-11 8.594157e-10 20 31757407 31757409 3 + 2.473 2.313 -0.533
ENSG00000088325 E021 690.9462562 0.0040449314 2.757791e-07 2.571391e-06 20 31757410 31757582 173 + 2.904 2.764 -0.465
ENSG00000088325 E022 687.6486815 0.0032610775 3.219886e-07 2.960148e-06 20 31760057 31760179 123 + 2.896 2.772 -0.412
ENSG00000088325 E023 675.0754621 0.0011143699 2.106503e-11 4.159586e-10 20 31766556 31766682 127 + 2.881 2.773 -0.362
ENSG00000088325 E024 241.5173205 0.0003616315 1.163125e-08 1.417136e-07 20 31770343 31770345 3 + 2.436 2.328 -0.360
ENSG00000088325 E025 592.6567808 0.0007865242 4.036126e-08 4.434963e-07 20 31770346 31770471 126 + 2.811 2.733 -0.258
ENSG00000088325 E026 553.0442535 0.0017475405 3.672723e-02 8.494834e-02 20 31771560 31771682 123 + 2.755 2.731 -0.077
ENSG00000088325 E027 643.2009493 0.0017890325 1.637365e-01 2.800066e-01 20 31775867 31775988 122 + 2.811 2.806 -0.017
ENSG00000088325 E028 727.1487417 0.0009408309 1.092595e-01 2.044597e-01 20 31777487 31777638 152 + 2.863 2.862 -0.002
ENSG00000088325 E029 758.0531578 0.0006526251 1.529733e-02 4.116173e-02 20 31778813 31778984 172 + 2.886 2.875 -0.038
ENSG00000088325 E030 5.0842783 0.0231358035 1.973687e-01 3.229340e-01 20 31780939 31781046 108 + 0.659 0.872 0.855
ENSG00000088325 E031 681.8104384 0.0009205026 9.655163e-02 1.851902e-01 20 31782249 31782390 142 + 2.835 2.833 -0.008
ENSG00000088325 E032 1001.3010623 0.0004635160 1.916091e-01 3.157737e-01 20 31783705 31783921 217 + 2.995 3.007 0.038
ENSG00000088325 E033 730.5393148 0.0001279040 3.284632e-01 4.719620e-01 20 31792735 31792830 96 + 2.856 2.874 0.059
ENSG00000088325 E034 441.9776637 0.0004528593 2.580618e-01 3.952673e-01 20 31793848 31793851 4 + 2.642 2.651 0.030
ENSG00000088325 E035 1066.7228351 0.0001086168 1.419496e-05 9.102331e-05 20 31793852 31794024 173 + 2.987 3.068 0.269
ENSG00000088325 E036 910.3394612 0.0008379977 1.022685e-05 6.782826e-05 20 31794402 31794548 147 + 2.903 3.013 0.365
ENSG00000088325 E037 890.5209196 0.0012762731 8.361215e-08 8.623263e-07 20 31797404 31797515 112 + 2.875 3.015 0.466
ENSG00000088325 E038 468.8827583 0.0009519573 1.375075e-04 6.926084e-04 20 31798365 31798385 21 + 2.612 2.726 0.380
ENSG00000088325 E039 1056.9645346 0.0030194223 9.067852e-07 7.597865e-06 20 31798386 31798552 167 + 2.933 3.101 0.559
ENSG00000088325 E040 2184.6388941 0.0046956233 3.846499e-15 1.347213e-13 20 31800970 31801805 836 + 3.181 3.455 0.911