ENSG00000088205

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000263239 ENSG00000088205 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX18 protein_coding protein_coding 58.06311 13.19488 99.93708 1.038793 3.96754 2.920093 38.021596 11.7370043 65.919771 1.0100513 3.3257711 2.488637 0.7194708 0.88856667 0.6606667 -0.22790000 2.894409e-07 5.799205e-37 FALSE TRUE
ENST00000415038 ENSG00000088205 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX18 protein_coding protein_coding 58.06311 13.19488 99.93708 1.038793 3.96754 2.920093 6.754616 0.0000000 9.576995 0.0000000 2.8954917 9.904935 0.0888375 0.00000000 0.0946000 0.09460000 1.858168e-07 5.799205e-37 FALSE FALSE
ENST00000474694 ENSG00000088205 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX18 protein_coding processed_transcript 58.06311 13.19488 99.93708 1.038793 3.96754 2.920093 7.627134 0.0000000 14.183798 0.0000000 0.9316204 10.471045 0.0785625 0.00000000 0.1416667 0.14166667 5.799205e-37 5.799205e-37 FALSE TRUE
MSTRG.19108.1 ENSG00000088205 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX18 protein_coding   58.06311 13.19488 99.93708 1.038793 3.96754 2.920093 3.221532 0.8928842 5.581896 0.2027828 0.3570103 2.630725 0.0562750 0.06803333 0.0560000 -0.01203333 7.931904e-01 5.799205e-37 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000088205 E001 0.1451727 0.0432200398 1.000000e+00   2 117814593 117814606 14 + 0.056 0.000 -8.009
ENSG00000088205 E002 17.8179525 0.0378840978 2.094699e-01 3.379011e-01 2 117814607 117814690 84 + 1.186 1.016 -0.613
ENSG00000088205 E003 363.6302928 0.0002156174 4.357881e-22 3.502704e-20 2 117814691 117814862 172 + 2.479 2.232 -0.825
ENSG00000088205 E004 0.1515154 0.0435644859 1.000000e+00   2 117815058 117815092 35 + 0.056 0.000 -7.988
ENSG00000088205 E005 0.2924217 0.0272881819 1.000000e+00   2 117815688 117815723 36 + 0.105 0.000 -8.987
ENSG00000088205 E006 596.7783139 0.0009572783 9.252954e-15 3.059240e-13 2 117817444 117817728 285 + 2.684 2.514 -0.567
ENSG00000088205 E007 405.9208592 0.0003959346 1.054067e-03 4.146554e-03 2 117819649 117819792 144 + 2.501 2.446 -0.183
ENSG00000088205 E008 383.2650384 0.0001252870 1.109850e-03 4.336132e-03 2 117821161 117821296 136 + 2.476 2.424 -0.172
ENSG00000088205 E009 329.5135441 0.0002599962 1.419200e-01 2.506602e-01 2 117821650 117821750 101 + 2.404 2.393 -0.036
ENSG00000088205 E010 377.1038545 0.0010799986 6.888534e-03 2.093546e-02 2 117821862 117821975 114 + 2.469 2.419 -0.168
ENSG00000088205 E011 362.6395866 0.0007114184 2.295208e-02 5.764553e-02 2 117821976 117822061 86 + 2.449 2.414 -0.118
ENSG00000088205 E012 394.5029148 0.0001588505 1.199294e-03 4.638021e-03 2 117822147 117822261 115 + 2.489 2.438 -0.169
ENSG00000088205 E013 20.6305962 0.0026937048 9.061076e-07 7.593195e-06 2 117822262 117822752 491 + 1.289 0.612 -2.577
ENSG00000088205 E014 20.7070119 0.0008632681 3.898591e-01 5.339081e-01 2 117822753 117822845 93 + 1.234 1.174 -0.213
ENSG00000088205 E015 14.3235981 0.0287221292 3.195263e-01 4.626088e-01 2 117824337 117824568 232 + 1.097 0.976 -0.445
ENSG00000088205 E016 455.8640190 0.0003984352 1.359622e-01 2.424294e-01 2 117824569 117824708 140 + 2.544 2.537 -0.023
ENSG00000088205 E017 278.3955133 0.0001542921 5.089455e-01 6.443258e-01 2 117824940 117824996 57 + 2.328 2.338 0.034
ENSG00000088205 E018 430.2968270 0.0004011476 1.086690e-01 2.035679e-01 2 117824997 117825101 105 + 2.519 2.509 -0.035
ENSG00000088205 E019 487.4325131 0.0003681459 1.030026e-01 1.950495e-01 2 117825447 117825599 153 + 2.574 2.566 -0.026
ENSG00000088205 E020 4.2187748 0.0039087371 8.698616e-03 2.553342e-02 2 117825600 117825983 384 + 0.522 0.941 1.733
ENSG00000088205 E021 348.0630660 0.0001578751 4.034939e-02 9.166433e-02 2 117826269 117826382 114 + 2.412 2.491 0.262
ENSG00000088205 E022 23.1520937 0.0046039413 8.278958e-01 8.918187e-01 2 117826383 117828948 2566 + 1.271 1.274 0.012
ENSG00000088205 E023 236.9910521 0.0003424981 5.022314e-05 2.829128e-04 2 117828949 117829005 57 + 2.231 2.378 0.490
ENSG00000088205 E024 3.5535840 0.0050598711 7.067959e-01 8.048790e-01 2 117829006 117829288 283 + 0.573 0.522 -0.236
ENSG00000088205 E025 305.6607218 0.0001433046 6.905256e-10 1.056006e-08 2 117829289 117829420 132 + 2.333 2.514 0.604
ENSG00000088205 E026 217.6998333 0.0003202866 3.107603e-07 2.865962e-06 2 117829421 117829466 46 + 2.186 2.367 0.606
ENSG00000088205 E027 1492.8132888 0.0027389086 1.403079e-11 2.851724e-10 2 117830582 117832377 1796 + 3.019 3.201 0.605