ENSG00000087995

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000311506 ENSG00000087995 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL2A protein_coding protein_coding 10.27829 8.190614 11.97599 1.417425 0.259109 0.5475453 5.8740596 4.3127825 6.9107957 0.98923911 0.33913255 0.6789777 0.55418333 0.51656667 0.57676667 0.06020000 0.6583708666 0.0001262559 FALSE TRUE
ENST00000333483 ENSG00000087995 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL2A protein_coding retained_intron 10.27829 8.190614 11.97599 1.417425 0.259109 0.5475453 2.9428588 2.8255566 3.4921906 0.47402773 0.20088893 0.3046257 0.30001667 0.35230000 0.29256667 -0.05973333 0.6797346636 0.0001262559 FALSE TRUE
ENST00000582027 ENSG00000087995 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL2A protein_coding retained_intron 10.27829 8.190614 11.97599 1.417425 0.259109 0.5475453 0.5809455 0.2358567 0.7716375 0.09046441 0.17522658 1.6686820 0.06117917 0.03406667 0.06390000 0.02983333 0.4635187031 0.0001262559   FALSE
MSTRG.14857.2 ENSG00000087995 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL2A protein_coding   10.27829 8.190614 11.97599 1.417425 0.259109 0.5475453 0.6276521 0.3036331 0.6489562 0.14024272 0.14453462 1.0711047 0.05786250 0.03383333 0.05396667 0.02013333 0.6769591803 0.0001262559 FALSE TRUE
MSTRG.14857.4 ENSG00000087995 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL2A protein_coding   10.27829 8.190614 11.97599 1.417425 0.259109 0.5475453 0.2422036 0.5127852 0.1524105 0.08815711 0.03212348 -1.6865734 0.02571667 0.06323333 0.01280000 -0.05043333 0.0001262559 0.0001262559 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000087995 E001 0.2987644 0.0293375050 2.176467e-01   17 62423875 62423876 2 + 0.219 0.000 -10.899
ENSG00000087995 E002 0.2987644 0.0293375050 2.176467e-01   17 62423877 62423877 1 + 0.219 0.000 -12.999
ENSG00000087995 E003 1.0371996 0.0202603134 4.578846e-03 1.476157e-02 17 62423878 62423889 12 + 0.517 0.000 -14.950
ENSG00000087995 E004 1.3671972 0.0123585730 9.705070e-02 1.859704e-01 17 62423890 62423890 1 + 0.517 0.217 -1.821
ENSG00000087995 E005 4.4252584 0.0066836270 1.103538e-01 2.060658e-01 17 62423891 62423896 6 + 0.858 0.627 -0.942
ENSG00000087995 E006 166.0406111 0.0069822032 9.070632e-01 9.450053e-01 17 62423897 62424012 116 + 2.236 2.214 -0.076
ENSG00000087995 E007 70.2922182 0.0127897611 9.818291e-01 9.926576e-01 17 62424013 62424218 206 + 1.869 1.838 -0.105
ENSG00000087995 E008 139.4370536 0.0004327921 7.278716e-03 2.194876e-02 17 62424219 62424310 92 + 2.202 2.107 -0.316
ENSG00000087995 E009 184.2406475 0.0017010038 2.823578e-02 6.847920e-02 17 62426299 62426654 356 + 2.318 2.233 -0.282
ENSG00000087995 E010 41.7113895 0.0014539395 2.954037e-01 4.365405e-01 17 62427788 62427837 50 + 1.615 1.662 0.159
ENSG00000087995 E011 4.3502059 0.0036762808 8.989467e-03 2.625374e-02 17 62434861 62435231 371 + 0.896 0.514 -1.598
ENSG00000087995 E012 82.3134033 0.0003846469 4.257900e-01 5.686868e-01 17 62435232 62435292 61 + 1.919 1.938 0.063
ENSG00000087995 E013 201.2452326 0.0016900808 9.804111e-01 9.917554e-01 17 62440617 62440740 124 + 2.319 2.303 -0.055
ENSG00000087995 E014 207.5765143 0.0002099745 5.078351e-03 1.614496e-02 17 62440741 62440756 16 + 2.295 2.350 0.184
ENSG00000087995 E015 322.4340856 0.0001653422 9.020229e-05 4.759645e-04 17 62444837 62444943 107 + 2.482 2.544 0.207
ENSG00000087995 E016 250.4974767 0.0001685481 3.886229e-06 2.834724e-05 17 62447701 62447766 66 + 2.358 2.447 0.295
ENSG00000087995 E017 141.1195317 0.0002994754 2.733148e-05 1.641578e-04 17 62448575 62448576 2 + 2.095 2.208 0.376
ENSG00000087995 E018 802.0663496 0.0012045012 7.179195e-06 4.930316e-05 17 62448577 62453385 4809 + 2.954 2.866 -0.294