ENSG00000087157

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262764 ENSG00000087157 HEK293_OSMI2_2hA HEK293_TMG_2hB PGS1 protein_coding protein_coding 31.59035 45.3259 19.32954 2.910157 0.752897 -1.229101 15.4885549 22.5616094 7.4810489 1.1510351 0.3532143 -1.5912697 0.47275417 0.49866667 0.3869333 -0.11173333 1.021819e-04 1.255719e-43 FALSE TRUE
ENST00000588281 ENSG00000087157 HEK293_OSMI2_2hA HEK293_TMG_2hB PGS1 protein_coding processed_transcript 31.59035 45.3259 19.32954 2.910157 0.752897 -1.229101 0.5294468 2.0258306 0.0000000 2.0258306 0.0000000 -7.6694737 0.01393333 0.05086667 0.0000000 -0.05086667 9.264298e-01 1.255719e-43 TRUE TRUE
ENST00000589426 ENSG00000087157 HEK293_OSMI2_2hA HEK293_TMG_2hB PGS1 protein_coding nonsense_mediated_decay 31.59035 45.3259 19.32954 2.910157 0.752897 -1.229101 2.4096793 5.0298262 0.4711686 0.6403924 0.4711686 -3.3887595 0.06836250 0.11023333 0.0249000 -0.08533333 1.117855e-01 1.255719e-43 TRUE TRUE
ENST00000589689 ENSG00000087157 HEK293_OSMI2_2hA HEK293_TMG_2hB PGS1 protein_coding protein_coding 31.59035 45.3259 19.32954 2.910157 0.752897 -1.229101 1.4259364 0.0000000 3.0811848 0.0000000 0.2856277 8.2720161 0.06043333 0.00000000 0.1593667 0.15936667 1.255719e-43 1.255719e-43 FALSE TRUE
ENST00000591996 ENSG00000087157 HEK293_OSMI2_2hA HEK293_TMG_2hB PGS1 protein_coding retained_intron 31.59035 45.3259 19.32954 2.910157 0.752897 -1.229101 4.1706072 4.1288461 3.3372196 0.4218425 0.1390628 -0.3062654 0.14081667 0.09076667 0.1735667 0.08280000 1.374322e-04 1.255719e-43 FALSE TRUE
MSTRG.15175.11 ENSG00000087157 HEK293_OSMI2_2hA HEK293_TMG_2hB PGS1 protein_coding   31.59035 45.3259 19.32954 2.910157 0.752897 -1.229101 1.9533666 3.5446716 0.0000000 1.7723633 0.0000000 -8.4735725 0.06128333 0.07380000 0.0000000 -0.07380000 2.417473e-01 1.255719e-43 TRUE TRUE
MSTRG.15175.7 ENSG00000087157 HEK293_OSMI2_2hA HEK293_TMG_2hB PGS1 protein_coding   31.59035 45.3259 19.32954 2.910157 0.752897 -1.229101 1.4254213 0.1468666 2.6462364 0.1468666 0.4793655 4.0817732 0.05251250 0.00370000 0.1354667 0.13176667 2.509182e-03 1.255719e-43 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000087157 E001 2.1274102 0.0073851431 9.583720e-01 9.779175e-01 17 78378649 78378650 2 + 0.425 0.435 0.056
ENSG00000087157 E002 9.0217377 0.0020374525 4.627429e-01 6.025991e-01 17 78378651 78378655 5 + 0.993 0.908 -0.321
ENSG00000087157 E003 10.0881046 0.0033973730 5.917771e-01 7.144630e-01 17 78378656 78378656 1 + 1.017 0.956 -0.225
ENSG00000087157 E004 16.6765518 0.0226030285 8.098239e-01 8.793012e-01 17 78378657 78378661 5 + 1.138 1.170 0.117
ENSG00000087157 E005 24.5924698 0.0346441768 7.926911e-01 8.674651e-01 17 78378662 78378669 8 + 1.343 1.319 -0.085
ENSG00000087157 E006 42.9202691 0.0316930516 9.498951e-01 9.726602e-01 17 78378670 78378677 8 + 1.549 1.563 0.051
ENSG00000087157 E007 56.4271675 0.0375375761 8.965432e-01 9.378578e-01 17 78378678 78378696 19 + 1.677 1.676 -0.003
ENSG00000087157 E008 127.1795663 0.0092946535 2.293076e-01 3.616098e-01 17 78378697 78378808 112 + 2.071 2.010 -0.203
ENSG00000087157 E009 0.2955422 0.0294762169 4.372905e-01   17 78380871 78381017 147 + 0.191 0.076 -1.533
ENSG00000087157 E010 11.6141084 0.0170660557 8.661423e-01 9.177968e-01 17 78382664 78382785 122 + 0.993 1.021 0.102
ENSG00000087157 E011 115.8430568 0.0041666831 4.100518e-02 9.288820e-02 17 78392476 78392513 38 + 2.052 1.960 -0.310
ENSG00000087157 E012 199.0319430 0.0041690526 3.007871e-02 7.210784e-02 17 78392514 78392665 152 + 2.279 2.197 -0.274
ENSG00000087157 E013 118.1269535 0.0028129859 5.848153e-01 7.086348e-01 17 78396308 78396385 78 + 1.970 1.998 0.093
ENSG00000087157 E014 191.1140445 0.0002221054 6.888367e-01 7.908924e-01 17 78398252 78398351 100 + 2.206 2.196 -0.033
ENSG00000087157 E015 20.5017398 0.0009748965 1.879401e-01 3.111299e-01 17 78399289 78399347 59 + 1.332 1.223 -0.382
ENSG00000087157 E016 293.3043924 0.0001904933 3.836223e-02 8.803432e-02 17 78399348 78399537 190 + 2.420 2.373 -0.157
ENSG00000087157 E017 147.8385606 0.0002702767 4.504533e-01 5.915447e-01 17 78400677 78400713 37 + 2.106 2.083 -0.079
ENSG00000087157 E018 122.4673194 0.0003790656 6.007442e-01 7.219294e-01 17 78400714 78400734 21 + 2.019 2.001 -0.059
ENSG00000087157 E019 196.0026796 0.0002696952 9.436202e-01 9.686328e-01 17 78400735 78400830 96 + 2.206 2.211 0.014
ENSG00000087157 E020 122.8301049 0.0010084526 1.790575e-02 4.693255e-02 17 78400831 78400855 25 + 1.930 2.030 0.336
ENSG00000087157 E021 376.7125664 0.0037050740 1.467812e-01 2.572929e-01 17 78403568 78403834 267 + 2.452 2.508 0.188
ENSG00000087157 E022 221.7528148 0.0005918165 9.140941e-01 9.495743e-01 17 78403835 78403929 95 + 2.266 2.266 -0.002
ENSG00000087157 E023 128.4819881 0.0002674846 4.565411e-01 5.969544e-01 17 78403930 78403963 34 + 2.048 2.023 -0.083
ENSG00000087157 E024 106.8363107 0.0003067586 9.408427e-01 9.668244e-01 17 78403964 78403975 12 + 1.949 1.948 -0.003
ENSG00000087157 E025 217.5708570 0.0001994616 4.970325e-01 6.336008e-01 17 78403976 78404089 114 + 2.238 2.259 0.071
ENSG00000087157 E026 146.8028133 0.0002400853 1.743418e-03 6.419142e-03 17 78414879 78414951 73 + 1.995 2.109 0.382
ENSG00000087157 E027 181.0343650 0.0002351139 1.890929e-02 4.913396e-02 17 78414952 78415027 76 + 2.115 2.192 0.257
ENSG00000087157 E028 249.6631543 0.0012745853 4.413693e-02 9.863761e-02 17 78419546 78419675 130 + 2.264 2.330 0.223
ENSG00000087157 E029 71.7169992 0.0003576884 7.851228e-03 2.340801e-02 17 78419676 78419725 50 + 1.869 1.748 -0.409
ENSG00000087157 E030 61.6367276 0.0004292177 1.254266e-13 3.514523e-12 17 78419726 78420221 496 + 1.955 1.609 -1.167
ENSG00000087157 E031 28.3359066 0.0012012653 5.443177e-04 2.325651e-03 17 78420222 78420323 102 + 1.561 1.321 -0.829
ENSG00000087157 E032 192.9927946 0.0037823088 5.667055e-13 1.446130e-11 17 78420324 78421911 1588 + 2.403 2.128 -0.918
ENSG00000087157 E033 299.4587467 0.0001622749 5.983341e-08 6.346212e-07 17 78424061 78424192 132 + 2.282 2.421 0.463
ENSG00000087157 E034 375.9918349 0.0065385864 3.069268e-05 1.821799e-04 17 78424193 78424659 467 + 2.344 2.532 0.626
ENSG00000087157 E035 3.6773403 0.0045167269 8.475012e-01 9.053067e-01 17 78424918 78425114 197 + 0.576 0.609 0.148