ENSG00000086619

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000354619 ENSG00000086619 HEK293_OSMI2_2hA HEK293_TMG_2hB ERO1B protein_coding protein_coding 5.900075 1.446783 11.52049 0.2190354 0.2460224 2.984596 2.8663845 1.09688278 5.7216013 0.24553619 0.19122775 2.3724358 0.54317083 0.7478667 0.49773333 -0.25013333 6.527973e-02 1.359331e-19 FALSE TRUE
ENST00000687487 ENSG00000086619 HEK293_OSMI2_2hA HEK293_TMG_2hB ERO1B protein_coding protein_coding 5.900075 1.446783 11.52049 0.2190354 0.2460224 2.984596 2.0107021 0.00000000 4.6575987 0.00000000 0.16082752 8.8665367 0.21571250 0.0000000 0.40410000 0.40410000 1.359331e-19 1.359331e-19 FALSE TRUE
ENST00000691519 ENSG00000086619 HEK293_OSMI2_2hA HEK293_TMG_2hB ERO1B protein_coding retained_intron 5.900075 1.446783 11.52049 0.2190354 0.2460224 2.984596 0.4197470 0.08790535 0.4862576 0.05210296 0.18535040 2.3416296 0.08455417 0.0541000 0.04203333 -0.01206667 8.325739e-01 1.359331e-19 FALSE TRUE
MSTRG.3327.1 ENSG00000086619 HEK293_OSMI2_2hA HEK293_TMG_2hB ERO1B protein_coding   5.900075 1.446783 11.52049 0.2190354 0.2460224 2.984596 0.2688111 0.11895529 0.2126669 0.06176724 0.08531413 0.7880159 0.05395000 0.0994000 0.01813333 -0.08126667 4.906708e-01 1.359331e-19 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000086619 E001 0.3666179 0.0296081266 1.991149e-01 0.3251130423 1 236214681 236215100 420 - 0.051 0.267 2.771
ENSG00000086619 E002 277.7533322 0.0127209820 2.133549e-05 0.0001314325 1 236215101 236218243 3143 - 2.252 2.510 0.861
ENSG00000086619 E003 36.7898703 0.0006354853 8.872985e-02 0.1731820032 1 236218244 236218576 333 - 1.405 1.593 0.645
ENSG00000086619 E004 0.6256415 0.0176171709 5.663843e-01 0.6933706234 1 236220173 236220831 659 - 0.138 0.267 1.183
ENSG00000086619 E005 35.9090699 0.0009580514 5.208606e-01 0.6548069344 1 236220832 236220965 134 - 1.408 1.522 0.394
ENSG00000086619 E006 43.0550089 0.0005018559 2.474454e-01 0.3829446008 1 236221924 236222010 87 - 1.506 1.487 -0.063
ENSG00000086619 E007 49.0193731 0.0131531935 7.797512e-02 0.1562248381 1 236225070 236225139 70 - 1.568 1.466 -0.347
ENSG00000086619 E008 82.4368270 0.0003500733 2.387453e-04 0.0011279762 1 236226269 236226515 247 - 1.796 1.655 -0.477
ENSG00000086619 E009 43.0699214 0.0005965264 8.096858e-02 0.1610521800 1 236226647 236226739 93 - 1.514 1.450 -0.221
ENSG00000086619 E010 2.2916918 0.0062332162 3.409734e-01 0.4849063381 1 236226740 236229165 2426 - 0.439 0.267 -1.039
ENSG00000086619 E011 0.1451727 0.0439358884 1.000000e+00   1 236229166 236229415 250 - 0.051 0.000 -9.426
ENSG00000086619 E012 0.5933762 0.0241121669 6.906875e-01 0.7923355314 1 236229416 236230223 808 - 0.176 0.000 -11.406
ENSG00000086619 E013 29.8006787 0.0006576203 4.195815e-01 0.5628417232 1 236230224 236230250 27 - 1.351 1.347 -0.015
ENSG00000086619 E014 2.0035485 0.0146054845 4.330520e-01 0.5755073289 1 236230251 236230605 355 - 0.352 0.550 1.030
ENSG00000086619 E015 24.9300211 0.0007385709 1.538730e-01 0.2669345585 1 236232828 236232839 12 - 1.286 1.212 -0.262
ENSG00000086619 E016 22.3961453 0.0008760662 1.551137e-01 0.2685550518 1 236235789 236235795 7 - 1.245 1.164 -0.288
ENSG00000086619 E017 27.3769005 0.0007360393 2.266739e-01 0.3584232761 1 236235796 236235835 40 - 1.321 1.275 -0.162
ENSG00000086619 E018 2.5725834 0.1921995045 1.753939e-01 0.2951829269 1 236235985 236236113 129 - 0.374 0.725 1.658
ENSG00000086619 E019 3.0907022 0.3046905620 1.735265e-01 0.2927697031 1 236236114 236236277 164 - 0.374 0.868 2.224
ENSG00000086619 E020 30.8825880 0.0007853660 6.737203e-02 0.1389852121 1 236236278 236236331 54 - 1.375 1.275 -0.347
ENSG00000086619 E021 37.4596125 0.0180381348 2.576371e-01 0.3948025979 1 236236332 236236398 67 - 1.448 1.398 -0.172
ENSG00000086619 E022 1.1146300 0.0212494776 3.524864e-01 0.4967343351 1 236242829 236242909 81 - 0.210 0.431 1.446
ENSG00000086619 E023 39.9710185 0.0007186858 3.451604e-01 0.4892387590 1 236243422 236243495 74 - 1.469 1.463 -0.020
ENSG00000086619 E024 34.0682749 0.0006672356 7.471105e-02 0.1510752231 1 236249885 236249967 83 - 1.416 1.330 -0.298
ENSG00000086619 E025 31.2421459 0.0006787283 4.296291e-01 0.5723322953 1 236252050 236252091 42 - 1.365 1.363 -0.007
ENSG00000086619 E026 36.9827755 0.0005965550 2.384645e-02 0.0594919387 1 236253422 236253505 84 - 1.454 1.330 -0.428
ENSG00000086619 E027 2.3356104 0.0067381838 5.788181e-01 0.7038031343 1 236265080 236269874 4795 - 0.397 0.550 0.768
ENSG00000086619 E028 34.1859891 0.0017119239 1.475195e-02 0.0399313194 1 236269875 236269994 120 - 1.428 1.275 -0.534
ENSG00000086619 E029 0.0000000       1 236275596 236275679 84 -      
ENSG00000086619 E030 0.4720498 0.2522313676 3.860728e-01 0.5302598014 1 236281467 236281681 215 - 0.096 0.268 1.776
ENSG00000086619 E031 31.5479606 0.0007795338 2.685843e-01 0.4070664903 1 236281682 236282019 338 - 1.377 1.347 -0.104