ENSG00000086598

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262225 ENSG00000086598 HEK293_OSMI2_2hA HEK293_TMG_2hB TMED2 protein_coding protein_coding 146.8594 79.64767 229.6967 13.98052 1.705485 1.527908 127.82065 76.467041 191.89157 13.9744077 7.031146 1.327268 0.89943750 0.95790000 0.8350667 -0.1228333 5.690328e-06 9.58959e-09 FALSE TRUE
ENST00000438031 ENSG00000086598 HEK293_OSMI2_2hA HEK293_TMG_2hB TMED2 protein_coding protein_coding 146.8594 79.64767 229.6967 13.98052 1.705485 1.527908 16.93508 2.226065 34.80705 0.1046492 5.550435 3.960760 0.08564583 0.02913333 0.1519333 0.1228000 9.589590e-09 9.58959e-09 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000086598 E001 15.682986 4.257910e-02 3.586704e-03 1.197346e-02 12 123584533 123584548 16 + 1.250 0.900 -1.273
ENSG00000086598 E002 45.414368 2.485921e-03 1.651563e-06 1.307714e-05 12 123584549 123584551 3 + 1.671 1.464 -0.707
ENSG00000086598 E003 290.146600 1.253807e-02 5.615004e-04 2.390279e-03 12 123584552 123584597 46 + 2.448 2.330 -0.393
ENSG00000086598 E004 1104.833395 6.574164e-03 1.150605e-06 9.432222e-06 12 123584598 123584816 219 + 3.023 2.925 -0.326
ENSG00000086598 E005 49.261916 5.546009e-04 7.414800e-05 4.000090e-04 12 123585562 123586746 1185 + 1.686 1.570 -0.395
ENSG00000086598 E006 1740.606955 7.430532e-04 3.842563e-25 4.259579e-23 12 123586747 123586939 193 + 3.209 3.161 -0.159
ENSG00000086598 E007 2.092038 2.351218e-01 1.339977e-01 2.397109e-01 12 123586940 123586960 21 + 0.284 0.712 2.169
ENSG00000086598 E008 0.599719 1.509617e-01 2.573917e-01 3.944960e-01 12 123587611 123587631 21 + 0.240 0.001 -8.941
ENSG00000086598 E009 1546.718427 8.298602e-05 1.562131e-23 1.467234e-21 12 123590342 123590449 108 + 3.143 3.157 0.046
ENSG00000086598 E010 1.139508 1.120873e-02 9.621023e-01 9.800984e-01 12 123590450 123590492 43 + 0.284 0.332 0.319
ENSG00000086598 E011 6661.954488 3.785467e-03 1.966337e-14 6.201376e-13 12 123596605 123598582 1978 + 3.712 3.911 0.662