ENSG00000086589

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000199814 ENSG00000086589 HEK293_OSMI2_2hA HEK293_TMG_2hB RBM22 protein_coding protein_coding 56.59422 50.44111 68.02485 3.553333 1.098011 0.4313879 43.680469 48.1905230 45.569978 3.54082604 0.0751713 -0.08064856 0.78682500 0.95496667 0.67023333 -0.28473333 4.951405e-51 4.951405e-51 FALSE TRUE
ENST00000520132 ENSG00000086589 HEK293_OSMI2_2hA HEK293_TMG_2hB RBM22 protein_coding retained_intron 56.59422 50.44111 68.02485 3.553333 1.098011 0.4313879 3.422992 1.2360656 4.885033 0.25866047 0.3191431 1.97393859 0.05915000 0.02426667 0.07190000 0.04763333 2.334677e-05 4.951405e-51 FALSE TRUE
ENST00000521248 ENSG00000086589 HEK293_OSMI2_2hA HEK293_TMG_2hB RBM22 protein_coding retained_intron 56.59422 50.44111 68.02485 3.553333 1.098011 0.4313879 2.827273 0.4834331 4.172383 0.24859751 0.1526183 3.08339881 0.04693333 0.01016667 0.06143333 0.05126667 1.749091e-01 4.951405e-51 FALSE TRUE
ENST00000522469 ENSG00000086589 HEK293_OSMI2_2hA HEK293_TMG_2hB RBM22 protein_coding retained_intron 56.59422 50.44111 68.02485 3.553333 1.098011 0.4313879 6.352888 0.4265086 12.928850 0.08020573 1.4262546 4.88955555 0.10178750 0.00860000 0.18950000 0.18090000 1.554334e-28 4.951405e-51 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000086589 E001 0.4417471 0.6883775827 3.244120e-01 4.677628e-01 5 150688939 150689045 107 - 0.278 0.000 -10.429
ENSG00000086589 E002 2.5702396 0.1544603776 6.712768e-02 1.385816e-01 5 150690792 150690793 2 - 0.279 0.719 2.238
ENSG00000086589 E003 122.0001117 0.0038346899 2.181776e-06 1.682532e-05 5 150690794 150690849 56 - 1.950 2.199 0.833
ENSG00000086589 E004 1197.9775937 0.0029408197 3.023355e-19 1.769980e-17 5 150690850 150691518 669 - 2.930 3.189 0.863
ENSG00000086589 E005 642.7519990 0.0017012547 2.841476e-08 3.213594e-07 5 150691519 150691881 363 - 2.718 2.887 0.562
ENSG00000086589 E006 314.2548392 0.0010099831 4.005260e-05 2.308160e-04 5 150692895 150693026 132 - 2.424 2.567 0.478
ENSG00000086589 E007 345.1280899 0.0004045406 2.996622e-02 7.189547e-02 5 150693219 150693307 89 - 2.497 2.584 0.292
ENSG00000086589 E008 390.5897966 0.0001757684 1.644272e-01 2.809391e-01 5 150694076 150694240 165 - 2.560 2.628 0.227
ENSG00000086589 E009 56.3305018 0.0014598181 1.887020e-30 3.282739e-28 5 150694241 150694833 593 - 1.963 1.380 -1.983
ENSG00000086589 E010 21.2241141 0.0008664546 2.800985e-18 1.458599e-16 5 150695324 150695505 182 - 1.570 0.868 -2.505
ENSG00000086589 E011 511.9192793 0.0006860786 7.596469e-03 2.276237e-02 5 150695506 150695693 188 - 2.717 2.706 -0.037
ENSG00000086589 E012 222.5506219 0.0015844258 6.480327e-03 1.987242e-02 5 150695694 150695706 13 - 2.371 2.328 -0.143
ENSG00000086589 E013 331.1322458 0.0018815571 4.387991e-04 1.923797e-03 5 150696533 150696594 62 - 2.552 2.492 -0.199
ENSG00000086589 E014 326.7811450 0.0013549756 9.911295e-05 5.176910e-04 5 150696595 150696666 72 - 2.547 2.487 -0.201
ENSG00000086589 E015 244.3438033 0.0001819303 6.293788e-06 4.381184e-05 5 150696667 150696705 39 - 2.422 2.362 -0.201
ENSG00000086589 E016 12.7430766 0.0038691731 9.744995e-11 1.722325e-09 5 150696706 150696790 85 - 1.353 0.690 -2.466
ENSG00000086589 E017 297.0128422 0.0001540033 9.362557e-04 3.741022e-03 5 150696791 150696891 101 - 2.490 2.465 -0.085
ENSG00000086589 E018 369.6832024 0.0001627902 2.837087e-09 3.868694e-08 5 150698499 150698631 133 - 2.605 2.536 -0.227
ENSG00000086589 E019 201.0014799 0.0002674792 5.243334e-09 6.815794e-08 5 150699242 150699255 14 - 2.359 2.252 -0.356
ENSG00000086589 E020 212.6675076 0.0015230997 6.469520e-06 4.489985e-05 5 150699256 150699271 16 - 2.377 2.280 -0.324
ENSG00000086589 E021 247.2611018 0.0022061650 8.700273e-06 5.861662e-05 5 150700444 150700476 33 - 2.444 2.342 -0.340
ENSG00000086589 E022 229.8008634 0.0010860833 3.072185e-07 2.836199e-06 5 150700477 150700497 21 - 2.413 2.312 -0.339
ENSG00000086589 E023 2.1808727 0.0068192133 1.385198e-01 2.460426e-01 5 150700498 150700910 413 - 0.601 0.383 -1.079
ENSG00000086589 E024 292.1030870 0.0005034727 1.119262e-11 2.312583e-10 5 150700932 150701077 146 - 2.522 2.409 -0.378