ENSG00000086232

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000199389 ENSG00000086232 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2AK1 protein_coding protein_coding 88.1136 69.0702 104.1777 2.419359 2.062786 0.5928412 22.32217 26.594584 23.55520 1.284088 1.0493802 -0.1750169 0.2674125 0.3850333 0.2259000 -0.1591333 1.819132e-11 1.819132e-11 FALSE TRUE
MSTRG.29457.1 ENSG00000086232 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2AK1 protein_coding   88.1136 69.0702 104.1777 2.419359 2.062786 0.5928412 13.33299 14.449339 11.36844 2.394895 1.5672715 -0.3456985 0.1592792 0.2106333 0.1097333 -0.1009000 1.098665e-01 1.819132e-11 TRUE TRUE
MSTRG.29457.4 ENSG00000086232 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2AK1 protein_coding   88.1136 69.0702 104.1777 2.419359 2.062786 0.5928412 22.03397 2.375889 34.96472 2.271349 0.9759868 3.8737137 0.2228208 0.0326000 0.3359000 0.3033000 2.794077e-02 1.819132e-11 FALSE TRUE
MSTRG.29457.5 ENSG00000086232 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2AK1 protein_coding   88.1136 69.0702 104.1777 2.419359 2.062786 0.5928412 27.88702 23.622255 31.97938 1.836956 2.5168658 0.4368356 0.3180667 0.3419667 0.3062667 -0.0357000 6.687879e-01 1.819132e-11 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000086232 E001 18.8880494 2.570319e-02 9.468502e-03 2.745889e-02 7 6018905 6019313 409 - 1.116 1.428 1.096
ENSG00000086232 E002 123.6161066 2.793201e-04 2.653527e-08 3.019417e-07 7 6022247 6022786 540 - 1.984 2.187 0.681
ENSG00000086232 E003 127.0316910 2.852052e-04 1.906313e-01 3.145454e-01 7 6022787 6023175 389 - 2.075 2.139 0.214
ENSG00000086232 E004 160.0418776 6.616883e-04 7.858354e-02 1.572227e-01 7 6023176 6023721 546 - 2.224 2.192 -0.105
ENSG00000086232 E005 40.5888874 8.057229e-04 4.005194e-02 9.113175e-02 7 6023722 6023746 25 - 1.667 1.575 -0.313
ENSG00000086232 E006 1700.3537328 4.208338e-03 1.390292e-11 2.828768e-10 7 6023747 6024284 538 - 3.101 3.328 0.757
ENSG00000086232 E007 752.8689907 3.932030e-03 5.840390e-07 5.101172e-06 7 6024285 6024355 71 - 2.774 2.959 0.616
ENSG00000086232 E008 1354.5604790 1.457243e-03 2.853044e-14 8.771512e-13 7 6024356 6024708 353 - 3.037 3.209 0.570
ENSG00000086232 E009 597.9207974 1.269611e-04 1.443003e-08 1.726818e-07 7 6024709 6024801 93 - 2.721 2.829 0.358
ENSG00000086232 E010 5.1369372 3.245459e-03 2.681913e-01 4.066302e-01 7 6026435 6026727 293 - 0.846 0.718 -0.512
ENSG00000086232 E011 1013.9760164 1.114151e-04 1.973242e-11 3.913576e-10 7 6026728 6026961 234 - 2.954 3.054 0.335
ENSG00000086232 E012 707.8307427 8.678912e-05 8.923740e-01 9.352245e-01 7 6028615 6028697 83 - 2.842 2.862 0.067
ENSG00000086232 E013 1.4917433 1.772595e-01 5.314672e-01 6.639030e-01 7 6028698 6028839 142 - 0.449 0.316 -0.761
ENSG00000086232 E014 792.3444322 9.651838e-05 6.766190e-01 7.815492e-01 7 6028918 6029032 115 - 2.892 2.909 0.055
ENSG00000086232 E015 676.7568884 6.408800e-04 4.441524e-01 5.857320e-01 7 6037424 6037524 101 - 2.828 2.834 0.021
ENSG00000086232 E016 713.3170657 7.686937e-04 9.736021e-02 1.864409e-01 7 6038560 6038671 112 - 2.860 2.849 -0.036
ENSG00000086232 E017 622.2088434 1.162759e-04 4.162864e-09 5.500556e-08 7 6040892 6040958 67 - 2.828 2.764 -0.215
ENSG00000086232 E018 449.6301586 1.175042e-03 1.627604e-04 8.038393e-04 7 6040959 6040975 17 - 2.688 2.622 -0.221
ENSG00000086232 E019 897.1544358 7.349672e-04 1.572444e-06 1.251028e-05 7 6040976 6041152 177 - 2.985 2.924 -0.205
ENSG00000086232 E020 489.9730507 1.335821e-04 5.339861e-09 6.930808e-08 7 6041153 6041219 67 - 2.729 2.654 -0.249
ENSG00000086232 E021 453.5495983 1.420991e-04 2.605211e-09 3.577482e-08 7 6042933 6042990 58 - 2.697 2.617 -0.269
ENSG00000086232 E022 252.0912589 1.782788e-04 1.643224e-07 1.601110e-06 7 6042991 6042993 3 - 2.451 2.354 -0.326
ENSG00000086232 E023 566.0662344 1.279533e-04 1.200729e-07 1.201725e-06 7 6044562 6044661 100 - 2.785 2.726 -0.199
ENSG00000086232 E024 550.7017048 6.919351e-04 6.694704e-08 7.033170e-07 7 6046071 6046151 81 - 2.784 2.700 -0.280
ENSG00000086232 E025 2.4766424 1.136108e-01 3.267370e-01 4.701919e-01 7 6046152 6046437 286 - 0.635 0.438 -0.925
ENSG00000086232 E026 3.8672774 6.037813e-02 6.486754e-02 1.348169e-01 7 6046723 6046776 54 - 0.492 0.838 1.481
ENSG00000086232 E027 13.1722934 2.667779e-03 7.935741e-02 1.584998e-01 7 6046777 6046991 215 - 1.051 1.235 0.661
ENSG00000086232 E028 593.4989806 3.439129e-03 9.635716e-04 3.833948e-03 7 6046992 6047091 100 - 2.816 2.730 -0.285
ENSG00000086232 E029 0.6633060 1.865128e-02 3.676794e-01 5.120316e-01 7 6047204 6047246 43 - 0.279 0.131 -1.358
ENSG00000086232 E030 351.9629348 3.627659e-03 1.738930e-03 6.404755e-03 7 6048807 6048844 38 - 2.592 2.501 -0.301
ENSG00000086232 E031 0.4513240 2.005484e-01 6.688336e-01 7.757491e-01 7 6049784 6049911 128 - 0.205 0.130 -0.795
ENSG00000086232 E032 687.8297019 2.574357e-03 4.685321e-08 5.083735e-07 7 6049912 6050045 134 - 2.900 2.767 -0.443
ENSG00000086232 E033 629.5210182 2.933142e-03 3.167384e-10 5.137427e-09 7 6054546 6054704 159 - 2.877 2.709 -0.559
ENSG00000086232 E034 0.6674587 1.906820e-02 3.670537e-01 5.114196e-01 7 6058148 6058242 95 - 0.279 0.131 -1.358
ENSG00000086232 E035 344.8385461 9.509513e-03 1.010050e-04 5.265721e-04 7 6058966 6059201 236 - 2.621 2.439 -0.605