ENSG00000085999

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000371975 ENSG00000085999 HEK293_OSMI2_2hA HEK293_TMG_2hB RAD54L protein_coding protein_coding 54.76392 56.6659 45.45281 0.4299397 2.235332 -0.3180488 21.596097 20.473669 20.070500 0.9988702 0.2493696 -0.02867895 0.40804583 0.36163333 0.44320000 0.08156667 0.125402799 0.005411772 FALSE  
ENST00000459678 ENSG00000085999 HEK293_OSMI2_2hA HEK293_TMG_2hB RAD54L protein_coding nonsense_mediated_decay 54.76392 56.6659 45.45281 0.4299397 2.235332 -0.3180488 3.508679 5.911380 1.436684 1.2418913 0.4587540 -2.03318393 0.06128333 0.10413333 0.03176667 -0.07236667 0.015906018 0.005411772 FALSE  
ENST00000488942 ENSG00000085999 HEK293_OSMI2_2hA HEK293_TMG_2hB RAD54L protein_coding protein_coding 54.76392 56.6659 45.45281 0.4299397 2.235332 -0.3180488 5.999917 7.630863 3.880490 2.0462372 1.3615007 -0.97378398 0.10142917 0.13413333 0.08290000 -0.05123333 0.624674063 0.005411772 FALSE  
ENST00000664182 ENSG00000085999 HEK293_OSMI2_2hA HEK293_TMG_2hB RAD54L protein_coding retained_intron 54.76392 56.6659 45.45281 0.4299397 2.235332 -0.3180488 4.412298 2.852595 3.779907 0.1715147 0.8537538 0.40483871 0.07645000 0.05036667 0.08173333 0.03136667 0.216048727 0.005411772 FALSE  
ENST00000668565 ENSG00000085999 HEK293_OSMI2_2hA HEK293_TMG_2hB RAD54L protein_coding retained_intron 54.76392 56.6659 45.45281 0.4299397 2.235332 -0.3180488 5.186709 2.260418 4.361770 0.4325620 0.4880960 0.94525968 0.09356667 0.03993333 0.09546667 0.05553333 0.005411772 0.005411772 FALSE  
ENST00000671528 ENSG00000085999 HEK293_OSMI2_2hA HEK293_TMG_2hB RAD54L protein_coding protein_coding 54.76392 56.6659 45.45281 0.4299397 2.235332 -0.3180488 3.636145 7.736134 0.000000 3.8919195 0.0000000 -9.59733265 0.06089167 0.13706667 0.00000000 -0.13706667 0.161316381 0.005411772 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000085999 E001 0.1817044 0.0403531155 7.015580e-01   1 46246461 46246596 136 + 0.000 0.104 9.307
ENSG00000085999 E002 0.1482932 0.0415689621 7.033420e-01   1 46247069 46247240 172 + 0.000 0.103 9.313
ENSG00000085999 E003 0.1817044 0.0403531155 7.015580e-01   1 46247241 46247304 64 + 0.000 0.104 9.317
ENSG00000085999 E004 0.0000000       1 46247581 46247583 3 +      
ENSG00000085999 E005 0.4449813 0.0216316385 3.529639e-01 4.972223e-01 1 46247584 46247637 54 + 0.252 0.103 -1.552
ENSG00000085999 E006 1.6983250 0.0147881304 9.875129e-01 9.962794e-01 1 46247638 46247687 50 + 0.411 0.417 0.036
ENSG00000085999 E007 1.4016369 0.0359167066 3.998553e-01 5.435818e-01 1 46247688 46247694 7 + 0.252 0.418 1.038
ENSG00000085999 E008 3.3130037 0.0048782005 3.627358e-01 5.071566e-01 1 46247695 46247699 5 + 0.693 0.567 -0.549
ENSG00000085999 E009 5.7432308 0.0054234691 7.151509e-01 8.111570e-01 1 46247700 46247714 15 + 0.839 0.805 -0.135
ENSG00000085999 E010 11.9088559 0.0014945083 2.070915e-01 3.350492e-01 1 46247715 46247746 32 + 1.158 1.050 -0.389
ENSG00000085999 E011 11.9515692 0.0406717843 8.214511e-02 1.629066e-01 1 46247747 46247762 16 + 1.213 1.001 -0.766
ENSG00000085999 E012 17.1969174 0.0160530142 1.524901e-02 4.105038e-02 1 46247763 46247768 6 + 1.370 1.139 -0.815
ENSG00000085999 E013 67.4531727 0.0010214258 2.958281e-03 1.013945e-02 1 46247769 46247796 28 + 1.891 1.772 -0.402
ENSG00000085999 E014 70.0997761 0.0026217816 5.254405e-03 1.662238e-02 1 46247797 46247801 5 + 1.908 1.787 -0.407
ENSG00000085999 E015 269.0844560 0.0011316672 1.618446e-07 1.579390e-06 1 46247802 46248237 436 + 2.488 2.363 -0.416
ENSG00000085999 E016 164.2419191 0.0002688489 2.702091e-06 2.043444e-05 1 46248238 46248317 80 + 2.271 2.152 -0.395
ENSG00000085999 E017 135.4695131 0.0005946749 3.004240e-05 1.787626e-04 1 46248318 46248343 26 + 2.190 2.071 -0.399
ENSG00000085999 E018 149.7355013 0.0005227211 1.138629e-04 5.855315e-04 1 46248344 46248398 55 + 2.223 2.120 -0.346
ENSG00000085999 E019 102.2225994 0.0048733696 1.271573e-01 2.301719e-01 1 46248399 46248408 10 + 2.032 1.972 -0.202
ENSG00000085999 E020 202.1672142 0.0002497505 2.050833e-03 7.383582e-03 1 46248512 46248598 87 + 2.330 2.266 -0.215
ENSG00000085999 E021 2.7907177 0.0640154346 6.356639e-01 7.498840e-01 1 46248599 46248623 25 + 0.617 0.534 -0.377
ENSG00000085999 E022 250.0575563 0.0002303568 1.110547e-05 7.302159e-05 1 46250000 46250119 120 + 2.437 2.350 -0.289
ENSG00000085999 E023 0.6998377 0.0167331230 6.838340e-01 7.869323e-01 1 46257742 46257817 76 + 0.252 0.187 -0.548
ENSG00000085999 E024 144.0007803 0.0050092927 9.540602e-03 2.763752e-02 1 46258686 46258706 21 + 2.208 2.109 -0.334
ENSG00000085999 E025 175.1196200 0.0003804289 6.208924e-03 1.916485e-02 1 46258707 46258746 40 + 2.270 2.208 -0.206
ENSG00000085999 E026 213.4873623 0.0027677546 1.398010e-02 3.818082e-02 1 46259964 46260091 128 + 2.359 2.287 -0.239
ENSG00000085999 E027 87.9408602 0.0105194035 6.763642e-02 1.394259e-01 1 46260092 46260099 8 + 1.995 1.896 -0.333
ENSG00000085999 E028 6.4216078 0.0026257430 2.006919e-05 1.244329e-04 1 46260100 46260103 4 + 1.108 0.598 -1.997
ENSG00000085999 E029 16.6620457 0.0012479176 2.690616e-09 3.686744e-08 1 46260104 46260244 141 + 1.468 0.983 -1.719
ENSG00000085999 E030 142.8774202 0.0002674099 1.167013e-01 2.152517e-01 1 46260542 46260611 70 + 2.163 2.130 -0.110
ENSG00000085999 E031 214.8866504 0.0006254316 1.285639e-01 2.321624e-01 1 46260727 46260847 121 + 2.331 2.305 -0.087
ENSG00000085999 E032 133.2539156 0.0015357488 7.078676e-01 8.056527e-01 1 46260848 46260858 11 + 2.107 2.107 0.003
ENSG00000085999 E033 322.9539473 0.0002519119 4.743090e-02 1.045997e-01 1 46260859 46261015 157 + 2.509 2.482 -0.089
ENSG00000085999 E034 2.0898490 0.0076836402 3.136910e-01 4.563086e-01 1 46261016 46261074 59 + 0.575 0.417 -0.772
ENSG00000085999 E035 125.3641144 0.0002640417 6.332357e-01 7.479743e-01 1 46261261 46261263 3 + 2.064 2.095 0.103
ENSG00000085999 E036 221.4773929 0.0007133378 8.831022e-01 9.291922e-01 1 46261264 46261337 74 + 2.321 2.332 0.035
ENSG00000085999 E037 188.6889208 0.0002368641 8.640671e-01 9.163397e-01 1 46261338 46261385 48 + 2.254 2.264 0.034
ENSG00000085999 E038 206.2689022 0.0002354839 9.824436e-01 9.930743e-01 1 46267459 46267504 46 + 2.289 2.304 0.052
ENSG00000085999 E039 153.5624102 0.0013285230 1.899024e-01 3.136350e-01 1 46267505 46267508 4 + 2.189 2.160 -0.098
ENSG00000085999 E040 266.5561026 0.0002189371 4.325346e-01 5.749707e-01 1 46267509 46267609 101 + 2.411 2.408 -0.009
ENSG00000085999 E041 280.6199186 0.0001615324 9.655318e-01 9.823373e-01 1 46270659 46270785 127 + 2.423 2.439 0.054
ENSG00000085999 E042 1.8445575 0.0073266196 6.338428e-02 1.323323e-01 1 46271368 46271442 75 + 0.618 0.317 -1.551
ENSG00000085999 E043 176.4139466 0.0010578792 8.914737e-01 9.346981e-01 1 46272466 46272504 39 + 2.223 2.233 0.034
ENSG00000085999 E044 190.4322615 0.0025763656 2.914944e-01 4.323674e-01 1 46272505 46272540 36 + 2.230 2.282 0.172
ENSG00000085999 E045 324.3859979 0.0001970895 1.506398e-01 2.625463e-01 1 46272672 46272802 131 + 2.465 2.510 0.150
ENSG00000085999 E046 303.1802436 0.0011257978 1.566095e-03 5.849533e-03 1 46273355 46273465 111 + 2.400 2.498 0.327
ENSG00000085999 E047 40.9104877 0.0007524390 1.161411e-09 1.700092e-08 1 46273466 46273593 128 + 1.779 1.458 -1.092
ENSG00000085999 E048 31.8993926 0.0007065875 1.087598e-03 4.261473e-03 1 46273594 46273623 30 + 1.610 1.424 -0.638
ENSG00000085999 E049 347.8022632 0.0005204154 2.268323e-06 1.743314e-05 1 46273624 46273747 124 + 2.442 2.563 0.401
ENSG00000085999 E050 280.9556967 0.0004188617 8.294964e-13 2.064174e-11 1 46274138 46274216 79 + 2.304 2.493 0.630
ENSG00000085999 E051 419.6099818 0.0014958455 9.450983e-07 7.880547e-06 1 46274538 46274717 180 + 2.512 2.653 0.471
ENSG00000085999 E052 477.0259997 0.0032082181 7.376330e-07 6.305494e-06 1 46277817 46277980 164 + 2.544 2.717 0.576
ENSG00000085999 E053 490.8046337 0.0068453743 1.426363e-04 7.148872e-04 1 46278072 46278480 409 + 2.552 2.731 0.594