ENSG00000085978

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000392017 ENSG00000085978 HEK293_OSMI2_2hA HEK293_TMG_2hB ATG16L1 protein_coding protein_coding 18.5773 17.40384 17.89836 1.168702 0.4090592 0.04039873 2.9474876 3.1613892 2.65060737 0.24629162 0.85754567 -0.2533593 0.16199583 0.182700000 0.150300000 -0.03240000 7.797695e-01 1.169569e-20 FALSE TRUE
ENST00000392020 ENSG00000085978 HEK293_OSMI2_2hA HEK293_TMG_2hB ATG16L1 protein_coding protein_coding 18.5773 17.40384 17.89836 1.168702 0.4090592 0.04039873 6.0718347 5.9671275 6.94661711 0.65175589 0.55435168 0.2189336 0.32622917 0.344000000 0.387633333 0.04363333 7.217703e-01 1.169569e-20 FALSE TRUE
ENST00000473865 ENSG00000085978 HEK293_OSMI2_2hA HEK293_TMG_2hB ATG16L1 protein_coding retained_intron 18.5773 17.40384 17.89836 1.168702 0.4090592 0.04039873 2.9356593 5.9962243 0.02859121 1.03234433 0.02859121 -7.2820424 0.16554167 0.342700000 0.001633333 -0.34106667 1.169569e-20 1.169569e-20 FALSE FALSE
ENST00000479942 ENSG00000085978 HEK293_OSMI2_2hA HEK293_TMG_2hB ATG16L1 protein_coding retained_intron 18.5773 17.40384 17.89836 1.168702 0.4090592 0.04039873 3.3734640 0.8831971 4.43662319 0.15575195 0.57810787 2.3156597 0.17510417 0.052400000 0.247166667 0.19476667 6.250137e-06 1.169569e-20 TRUE TRUE
MSTRG.20082.8 ENSG00000085978 HEK293_OSMI2_2hA HEK293_TMG_2hB ATG16L1 protein_coding   18.5773 17.40384 17.89836 1.168702 0.4090592 0.04039873 0.7882895 0.1448398 1.21503643 0.07860024 0.35369088 2.9839760 0.04154583 0.008033333 0.068700000 0.06066667 9.987384e-03 1.169569e-20 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000085978 E001 0.0000000       2 233210051 233210079 29 +      
ENSG00000085978 E002 0.0000000       2 233210327 233210583 257 +      
ENSG00000085978 E003 1.8874068 0.0110542403 1.345253e-01 2.404375e-01 2 233251571 233251669 99 + 0.549 0.327 -1.175
ENSG00000085978 E004 2.6644620 0.0145304545 3.703951e-01 5.148263e-01 2 233251670 233251671 2 + 0.613 0.512 -0.467
ENSG00000085978 E005 2.8461664 0.0153749654 5.371547e-01 6.687939e-01 2 233251672 233251672 1 + 0.613 0.559 -0.244
ENSG00000085978 E006 3.1459750 0.0168991651 5.788388e-01 7.038239e-01 2 233251673 233251675 3 + 0.642 0.601 -0.179
ENSG00000085978 E007 4.6948766 0.0573258645 3.972318e-01 5.410053e-01 2 233251676 233251681 6 + 0.804 0.707 -0.392
ENSG00000085978 E008 8.5300577 0.0655217067 8.944520e-02 1.742841e-01 2 233251682 233251684 3 + 1.080 0.842 -0.890
ENSG00000085978 E009 20.7777161 0.0130013642 8.908258e-03 2.605312e-02 2 233251685 233251700 16 + 1.417 1.225 -0.671
ENSG00000085978 E010 21.8096146 0.0124260776 1.759843e-02 4.626594e-02 2 233251701 233251704 4 + 1.426 1.262 -0.571
ENSG00000085978 E011 21.8096146 0.0124260776 1.759843e-02 4.626594e-02 2 233251705 233251705 1 + 1.426 1.262 -0.571
ENSG00000085978 E012 24.2034223 0.0131476304 8.651543e-02 1.698282e-01 2 233251706 233251713 8 + 1.444 1.344 -0.349
ENSG00000085978 E013 76.0960092 0.0003215418 1.027295e-03 4.052854e-03 2 233251714 233251940 227 + 1.921 1.850 -0.241
ENSG00000085978 E014 25.5594582 0.0007539172 3.684812e-02 8.518115e-02 2 233251941 233251942 2 + 1.462 1.378 -0.289
ENSG00000085978 E015 71.0974917 0.0003711168 5.995844e-08 6.357501e-07 2 233256102 233256195 94 + 1.932 1.763 -0.567
ENSG00000085978 E016 98.0113716 0.0002942758 2.218735e-08 2.565194e-07 2 233263130 233263235 106 + 2.058 1.918 -0.470
ENSG00000085978 E017 86.9146899 0.0003711440 4.352824e-04 1.910181e-03 2 233263992 233264065 74 + 1.982 1.910 -0.242
ENSG00000085978 E018 0.8211642 0.1781550394 3.023267e-01 4.441980e-01 2 233264066 233264101 36 + 0.329 0.140 -1.576
ENSG00000085978 E019 145.9807439 0.0009740021 9.870567e-06 6.569755e-05 2 233264892 233265143 252 + 2.205 2.123 -0.275
ENSG00000085978 E020 0.2965864 0.1019041636 1.849854e-01   2 233265955 233266159 205 + 0.000 0.241 10.884
ENSG00000085978 E021 69.9773661 0.0113428281 3.111531e-01 4.535957e-01 2 233270002 233270067 66 + 1.856 1.841 -0.051
ENSG00000085978 E022 74.2095499 0.0034260322 6.712810e-02 1.385816e-01 2 233272966 233273052 87 + 1.892 1.861 -0.108
ENSG00000085978 E023 4.7233861 0.0135276245 1.626123e-06 1.289727e-05 2 233273053 233273720 668 + 0.976 0.243 -3.499
ENSG00000085978 E024 23.9374246 0.0030353189 3.114920e-01 4.539514e-01 2 233273721 233273777 57 + 1.408 1.391 -0.057
ENSG00000085978 E025 1.4854032 0.0088635716 8.631872e-04 3.484811e-03 2 233273991 233274041 51 + 0.582 0.000 -13.930
ENSG00000085978 E026 4.7800773 0.0075995441 1.636139e-05 1.035172e-04 2 233274304 233274675 372 + 0.950 0.328 -2.811
ENSG00000085978 E027 89.2081433 0.0082123382 5.523742e-02 1.183975e-01 2 233274676 233274778 103 + 1.981 1.934 -0.160
ENSG00000085978 E028 0.0000000       2 233275379 233275467 89 +      
ENSG00000085978 E029 0.4396707 0.0249802071 1.110763e-01 2.071214e-01 2 233277148 233277567 420 + 0.266 0.000 -12.165
ENSG00000085978 E030 76.3646434 0.0071284532 7.997089e-02 1.595166e-01 2 233277568 233277610 43 + 1.911 1.874 -0.125
ENSG00000085978 E031 82.6321523 0.0068270141 2.232354e-01 3.542487e-01 2 233277611 233277673 63 + 1.929 1.927 -0.005
ENSG00000085978 E032 64.3064372 0.0003690833 5.776325e-02 1.227959e-01 2 233281105 233281146 42 + 1.828 1.806 -0.075
ENSG00000085978 E033 57.7787146 0.0004435909 3.491843e-03 1.170126e-02 2 233281147 233281175 29 + 1.806 1.731 -0.252
ENSG00000085978 E034 81.4992686 0.0003428112 8.144587e-05 4.348225e-04 2 233282682 233282753 72 + 1.959 1.866 -0.315
ENSG00000085978 E035 0.5106578 0.2634331976 5.507588e-01 6.803389e-01 2 233282754 233282995 242 + 0.108 0.244 1.418
ENSG00000085978 E036 110.5005664 0.0003538299 7.793527e-02 1.561582e-01 2 233289854 233289970 117 + 2.048 2.049 0.004
ENSG00000085978 E037 42.7954980 0.0005119890 9.008125e-01 9.407471e-01 2 233289971 233289974 4 + 1.620 1.673 0.182
ENSG00000085978 E038 22.3112789 0.0039751649 3.304636e-09 4.448613e-08 2 233289975 233290106 132 + 1.521 1.088 -1.516
ENSG00000085978 E039 19.9954085 0.0081569653 8.069184e-07 6.835767e-06 2 233290107 233290247 141 + 1.462 1.062 -1.406
ENSG00000085978 E040 108.5173273 0.0003051871 7.325654e-01 8.240105e-01 2 233290248 233290353 106 + 2.016 2.065 0.164
ENSG00000085978 E041 64.8063413 0.0008180486 4.757856e-01 6.144669e-01 2 233292128 233292132 5 + 1.772 1.863 0.308
ENSG00000085978 E042 154.2390890 0.0002677485 4.004122e-02 9.111172e-02 2 233292133 233292277 145 + 2.132 2.250 0.397
ENSG00000085978 E043 101.9642495 0.0014674637 2.371739e-02 5.922939e-02 2 233292387 233292434 48 + 1.939 2.086 0.494
ENSG00000085978 E044 132.7194845 0.0026716089 5.669104e-03 1.773791e-02 2 233293256 233293357 102 + 2.041 2.207 0.556
ENSG00000085978 E045 702.0068203 0.0056279930 1.206081e-15 4.508455e-14 2 233294257 233295674 1418 + 2.677 2.980 1.006