ENSG00000085832

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000371733 ENSG00000085832 HEK293_OSMI2_2hA HEK293_TMG_2hB EPS15 protein_coding protein_coding 10.00805 3.660395 14.79228 0.2297927 0.9574086 2.011813 6.1621175 2.9638068 8.2215371 0.07133386 0.38655989 1.4688509 0.71380833 0.8146 0.55753333 -0.25706667 2.072771e-03 2.694478e-26 FALSE TRUE
MSTRG.1165.3 ENSG00000085832 HEK293_OSMI2_2hA HEK293_TMG_2hB EPS15 protein_coding   10.00805 3.660395 14.79228 0.2297927 0.9574086 2.011813 0.2388969 0.4043108 0.2661085 0.04111683 0.08962909 -0.5854782 0.04338333 0.1103 0.01846667 -0.09183333 3.785570e-04 2.694478e-26 FALSE TRUE
MSTRG.1165.4 ENSG00000085832 HEK293_OSMI2_2hA HEK293_TMG_2hB EPS15 protein_coding   10.00805 3.660395 14.79228 0.2297927 0.9574086 2.011813 3.3133762 0.0000000 5.9605088 0.00000000 0.69533915 9.2217101 0.20430833 0.0000 0.40083333 0.40083333 2.694478e-26 2.694478e-26 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000085832 E001 6.4296435 0.0085130589 1.234724e-05 8.031410e-05 1 51354263 51354355 93 - 0.583 1.198 2.386
ENSG00000085832 E002 357.9983005 0.0064728573 1.268225e-28 1.888847e-26 1 51354356 51356571 2216 - 2.320 2.791 1.570
ENSG00000085832 E003 104.0909508 0.0003328945 1.173765e-01 2.162023e-01 1 51356572 51356846 275 - 1.910 2.042 0.443
ENSG00000085832 E004 92.9345203 0.0069401267 9.858545e-01 9.951787e-01 1 51361171 51361355 185 - 1.877 1.953 0.256
ENSG00000085832 E005 87.3700408 0.0011360333 8.065257e-01 8.770521e-01 1 51363866 51364028 163 - 1.856 1.916 0.200
ENSG00000085832 E006 61.3959767 0.0004100304 3.004000e-01 4.420769e-01 1 51365953 51366029 77 - 1.714 1.729 0.050
ENSG00000085832 E007 0.0000000       1 51394212 51394380 169 -      
ENSG00000085832 E008 46.1235915 0.0005401436 4.067243e-01 5.503446e-01 1 51394381 51394418 38 - 1.591 1.610 0.064
ENSG00000085832 E009 42.1454058 0.0099495814 7.525812e-02 1.519448e-01 1 51394419 51394447 29 - 1.572 1.495 -0.262
ENSG00000085832 E010 0.0000000       1 51397111 51397211 101 -      
ENSG00000085832 E011 72.4521462 0.0095440995 2.954661e-02 7.106874e-02 1 51399032 51399165 134 - 1.802 1.718 -0.285
ENSG00000085832 E012 33.4559732 0.0006262514 6.409220e-02 1.335116e-01 1 51400918 51400953 36 - 1.471 1.402 -0.237
ENSG00000085832 E013 0.1515154 0.0429296056 1.000000e+00   1 51400954 51401199 246 - 0.063 0.000 -7.977
ENSG00000085832 E014 57.0927669 0.0004191468 5.982440e-01 7.198876e-01 1 51402435 51402525 91 - 1.677 1.718 0.139
ENSG00000085832 E015 36.5746439 0.0005456413 1.196765e-01 2.195083e-01 1 51403419 51403428 10 - 1.507 1.467 -0.137
ENSG00000085832 E016 59.3146646 0.0006112082 3.967552e-01 5.405264e-01 1 51403429 51403532 104 - 1.695 1.718 0.077
ENSG00000085832 E017 77.4743882 0.0117266323 7.691109e-01 8.508266e-01 1 51405905 51406108 204 - 1.803 1.843 0.135
ENSG00000085832 E018 82.7105534 0.0035779598 3.632498e-02 8.419888e-02 1 51408135 51408332 198 - 1.851 1.801 -0.169
ENSG00000085832 E019 91.6397244 0.0003052490 1.623682e-04 8.021321e-04 1 51409535 51409696 162 - 1.908 1.804 -0.349
ENSG00000085832 E020 63.1362681 0.0005160812 1.582696e-04 7.839694e-04 1 51421786 51421858 73 - 1.755 1.610 -0.492
ENSG00000085832 E021 0.5932625 0.2150052198 4.375245e-01 5.794884e-01 1 51421859 51422104 246 - 0.212 0.000 -9.977
ENSG00000085832 E022 56.5329533 0.0005598335 6.997536e-05 3.799112e-04 1 51440347 51440432 86 - 1.713 1.540 -0.586
ENSG00000085832 E023 71.3605040 0.0004717953 1.153665e-03 4.483527e-03 1 51444889 51445045 157 - 1.801 1.701 -0.339
ENSG00000085832 E024 68.1080572 0.0017131619 2.149569e-02 5.463599e-02 1 51446960 51447105 146 - 1.772 1.712 -0.201
ENSG00000085832 E025 8.9305948 0.0018810303 4.184128e-01 5.616761e-01 1 51447935 51448045 111 - 0.932 0.889 -0.162
ENSG00000085832 E026 54.1840349 0.0029272843 1.511372e-02 4.074466e-02 1 51448046 51448135 90 - 1.682 1.595 -0.297
ENSG00000085832 E027 1.3995737 0.0096765512 1.416293e-03 5.361102e-03 1 51457536 51458395 860 - 0.119 0.703 3.686
ENSG00000085832 E028 2.6580295 0.0080143353 1.167909e-03 4.531910e-03 1 51458396 51458733 338 - 0.322 0.888 2.611
ENSG00000085832 E029 0.5880631 0.4088587332 1.619456e-01 2.776600e-01 1 51459041 51459148 108 - 0.063 0.383 3.173
ENSG00000085832 E030 0.2924217 0.0290785164 1.000000e+00   1 51460944 51461090 147 - 0.119 0.000 -8.976
ENSG00000085832 E031 35.7258098 0.0239809773 3.018057e-02 7.232273e-02 1 51461091 51461122 32 - 1.519 1.371 -0.508
ENSG00000085832 E032 29.7346464 0.0350958267 3.188190e-02 7.562390e-02 1 51461123 51461150 28 - 1.447 1.273 -0.603
ENSG00000085832 E033 37.6206307 0.0065715594 1.558431e-03 5.824310e-03 1 51463673 51463749 77 - 1.547 1.352 -0.671
ENSG00000085832 E034 39.2720883 0.0006177596 5.536082e-06 3.901909e-05 1 51463750 51463798 49 - 1.574 1.298 -0.950
ENSG00000085832 E035 30.0830441 0.0006850883 5.475833e-04 2.337916e-03 1 51465261 51465266 6 - 1.455 1.235 -0.766
ENSG00000085832 E036 49.2135389 0.0004902597 1.445395e-03 5.457739e-03 1 51465267 51465326 60 - 1.647 1.514 -0.453
ENSG00000085832 E037 50.9553826 0.0004570161 1.328537e-03 5.070763e-03 1 51468473 51468568 96 - 1.662 1.532 -0.445
ENSG00000085832 E038 34.0413744 0.0108103405 5.041523e-02 1.099056e-01 1 51471690 51471737 48 - 1.491 1.377 -0.392
ENSG00000085832 E039 32.9615911 0.0224500433 2.670607e-02 6.538025e-02 1 51472859 51472948 90 - 1.486 1.300 -0.646
ENSG00000085832 E040 21.9518921 0.0018154575 5.063208e-04 2.183943e-03 1 51481273 51481314 42 - 1.331 1.046 -1.014
ENSG00000085832 E041 2.9246436 0.0104712586 1.419903e-01 2.507587e-01 1 51508689 51508771 83 - 0.460 0.756 1.320
ENSG00000085832 E042 19.7933243 0.0010360683 1.924887e-01 3.168842e-01 1 51519199 51519333 135 - 1.255 1.199 -0.195