ENSG00000085719

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000517490 ENSG00000085719 HEK293_OSMI2_2hA HEK293_TMG_2hB CPNE3 protein_coding protein_coding 40.92885 11.37168 70.76727 3.044197 3.859486 2.636573 32.9168055 9.540797 55.2165907 1.9816090 2.1662079 2.531670 0.84546667 0.86303333 0.781700000 -0.08133333 4.054420e-01 1.337256e-21 FALSE TRUE
ENST00000614678 ENSG00000085719 HEK293_OSMI2_2hA HEK293_TMG_2hB CPNE3 protein_coding retained_intron 40.92885 11.37168 70.76727 3.044197 3.859486 2.636573 0.2756825 1.013862 0.3306454 0.9673772 0.1208401 -1.587678 0.01371667 0.05996667 0.004866667 -0.05510000 6.394272e-01 1.337256e-21 FALSE TRUE
MSTRG.31771.2 ENSG00000085719 HEK293_OSMI2_2hA HEK293_TMG_2hB CPNE3 protein_coding   40.92885 11.37168 70.76727 3.044197 3.859486 2.636573 2.4860318 0.000000 5.6122337 0.0000000 0.4029463 9.135000 0.03594583 0.00000000 0.079200000 0.07920000 1.337256e-21 1.337256e-21 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000085719 E001 0.7490443 0.1112065900 5.521127e-01 6.814478e-01 8 86514435 86514436 2 + 0.195 0.000 -20.590
ENSG00000085719 E002 0.7490443 0.1112065900 5.521127e-01 6.814478e-01 8 86514437 86514437 1 + 0.195 0.000 -20.524
ENSG00000085719 E003 0.7490443 0.1112065900 5.521127e-01 6.814478e-01 8 86514438 86514438 1 + 0.195 0.000 -20.524
ENSG00000085719 E004 1.1929815 0.0193382976 2.471461e-01 3.825694e-01 8 86514439 86514442 4 + 0.279 0.000 -21.199
ENSG00000085719 E005 2.6868037 0.2304734395 9.252480e-02 1.790619e-01 8 86514443 86514445 3 + 0.483 0.000 -22.377
ENSG00000085719 E006 28.6498263 0.0006404428 1.615565e-04 7.986845e-04 8 86514446 86514458 13 + 1.329 1.089 -0.851
ENSG00000085719 E007 110.5244143 0.0003013936 3.941500e-08 4.341057e-07 8 86514459 86514503 45 + 1.888 1.765 -0.414
ENSG00000085719 E008 139.5821189 0.0008495487 1.608328e-09 2.294523e-08 8 86514504 86514541 38 + 1.989 1.859 -0.436
ENSG00000085719 E009 6.8511671 0.0024212694 4.064902e-01 5.501264e-01 8 86514542 86514561 20 + 0.750 0.755 0.020
ENSG00000085719 E010 6.1126182 0.0057674999 5.938891e-01 7.161986e-01 8 86514562 86514573 12 + 0.704 0.755 0.207
ENSG00000085719 E011 5.2498498 0.0030764380 8.961798e-01 9.376613e-01 8 86514574 86514722 149 + 0.642 0.755 0.471
ENSG00000085719 E012 2.8014206 0.0059371316 1.834842e-01 3.055710e-01 8 86515142 86515461 320 + 0.482 0.287 -1.114
ENSG00000085719 E013 148.8191968 0.0002848900 1.884911e-10 3.172166e-09 8 86515462 86515499 38 + 2.016 1.892 -0.416
ENSG00000085719 E014 0.8534295 0.0312827445 1.757423e-01 2.956557e-01 8 86521604 86521714 111 + 0.127 0.460 2.475
ENSG00000085719 E015 1.5229790 0.0123477366 6.793047e-01 7.835228e-01 8 86524709 86524814 106 + 0.279 0.459 1.055
ENSG00000085719 E016 7.9838403 0.0208455279 7.537784e-01 8.395864e-01 8 86524877 86524997 121 + 0.791 0.879 0.340
ENSG00000085719 E017 261.2682097 0.0002355733 1.151424e-12 2.795154e-11 8 86528536 86528677 142 + 2.253 2.182 -0.239
ENSG00000085719 E018 275.7301132 0.0002172517 1.977729e-11 3.921653e-10 8 86528945 86529062 118 + 2.274 2.225 -0.164
ENSG00000085719 E019 243.5318873 0.0010488842 3.071685e-11 5.906633e-10 8 86529063 86529124 62 + 2.224 2.133 -0.304
ENSG00000085719 E020 258.9771250 0.0068817464 3.747266e-04 1.676328e-03 8 86531155 86531229 75 + 2.245 2.203 -0.142
ENSG00000085719 E021 222.8793966 0.0010983434 1.256003e-04 6.393879e-04 8 86532509 86532580 72 + 2.174 2.192 0.061
ENSG00000085719 E022 239.6856066 0.0002255910 7.217632e-05 3.907632e-04 8 86537563 86537646 84 + 2.203 2.240 0.121
ENSG00000085719 E023 168.4792380 0.0002246317 2.719142e-05 1.634254e-04 8 86540245 86540274 30 + 2.056 2.055 -0.001
ENSG00000085719 E024 214.9591009 0.0002393765 1.940773e-07 1.864282e-06 8 86540275 86540334 60 + 2.163 2.143 -0.066
ENSG00000085719 E025 272.3256467 0.0001908143 8.766904e-10 1.313351e-08 8 86544740 86544838 99 + 2.266 2.237 -0.096
ENSG00000085719 E026 3.0907219 0.0122355352 1.993111e-02 5.134261e-02 8 86544839 86545333 495 + 0.527 0.000 -22.590
ENSG00000085719 E027 193.1946903 0.0002366795 8.778374e-11 1.564916e-09 8 86546595 86546627 33 + 2.125 2.037 -0.293
ENSG00000085719 E028 187.1344653 0.0002838093 3.484607e-07 3.180671e-06 8 86546628 86546681 54 + 2.104 2.073 -0.105
ENSG00000085719 E029 3.1355589 0.0081828723 1.393064e-01 2.471038e-01 8 86547306 86547710 405 + 0.513 0.288 -1.265
ENSG00000085719 E030 169.7758022 0.0004082372 3.203097e-04 1.462069e-03 8 86547711 86547770 60 + 2.056 2.080 0.079
ENSG00000085719 E031 242.0907919 0.0005225432 4.927899e-05 2.781230e-04 8 86548301 86548434 134 + 2.209 2.237 0.094
ENSG00000085719 E032 0.1451727 0.0444877713 1.000000e+00   8 86551005 86551045 41 + 0.046 0.000 -18.198
ENSG00000085719 E033 177.4287584 0.0002579896 7.047759e-03 2.134882e-02 8 86551046 86551100 55 + 2.071 2.137 0.222
ENSG00000085719 E034 1.6284995 0.0082490930 1.279200e-01 2.312675e-01 8 86551101 86551182 82 + 0.350 0.000 -21.658
ENSG00000085719 E035 176.3106983 0.0002055125 3.577007e-03 1.194692e-02 8 86551183 86551234 52 + 2.068 2.125 0.191
ENSG00000085719 E036 1.6930170 0.0359950641 7.813791e-01 8.595497e-01 8 86551235 86551512 278 + 0.304 0.459 0.888
ENSG00000085719 E037 243.8949009 0.0001773605 4.503387e-03 1.455247e-02 8 86554851 86554984 134 + 2.205 2.282 0.258
ENSG00000085719 E038 309.8012382 0.0001655317 2.041488e-03 7.354969e-03 8 86556102 86556338 237 + 2.309 2.391 0.273
ENSG00000085719 E039 0.2924217 0.0275776038 1.000000e+00   8 86557195 86557222 28 + 0.088 0.000 -19.198
ENSG00000085719 E040 2617.1851490 0.0131654354 4.601915e-10 7.252467e-09 8 86558288 86561498 3211 + 3.174 3.541 1.217