ENSG00000085491

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000370041 ENSG00000085491 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC25A24 protein_coding protein_coding 10.99886 3.857584 21.55068 0.9686552 0.8965605 2.478898 7.1958356 3.18534770 13.1238531 0.93084126 0.566832452 2.0392454 0.68656250 0.81053333 0.6089333 -0.20160000 7.777806e-05 1.80271e-28 FALSE TRUE
ENST00000569674 ENSG00000085491 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC25A24 protein_coding protein_coding 10.99886 3.857584 21.55068 0.9686552 0.8965605 2.478898 0.1400545 0.21171754 0.1062021 0.05045821 0.006252496 -0.9320863 0.04149583 0.06143333 0.0049000 -0.05653333 1.715219e-05 1.80271e-28 FALSE TRUE
MSTRG.1719.5 ENSG00000085491 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC25A24 protein_coding   10.99886 3.857584 21.55068 0.9686552 0.8965605 2.478898 3.3739539 0.03385226 7.9234758 0.02176574 0.468153388 7.4991582 0.20534583 0.01026667 0.3674000 0.35713333 1.802710e-28 1.80271e-28 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000085491 E001 3.6307102 0.1053790504 3.768204e-02 8.673320e-02 1 108134043 108134819 777 - 0.445 0.926 2.057
ENSG00000085491 E002 0.7414659 0.4087919473 8.645497e-03 2.539874e-02 1 108134820 108134822 3 - 0.000 0.688 13.925
ENSG00000085491 E003 2.3247200 0.1540004276 5.241904e-05 2.938612e-04 1 108134823 108134868 46 - 0.098 1.052 5.341
ENSG00000085491 E004 377.3183978 0.0064374293 1.594844e-28 2.356774e-26 1 108134869 108136483 1615 - 2.321 2.808 1.621
ENSG00000085491 E005 151.1966540 0.0002305996 3.864500e-02 8.852482e-02 1 108136484 108136837 354 - 2.042 2.082 0.136
ENSG00000085491 E006 36.8374353 0.0005437219 2.502523e-01 3.862348e-01 1 108139058 108139082 25 - 1.440 1.467 0.092
ENSG00000085491 E007 86.5871865 0.0003018185 6.651593e-02 1.375817e-01 1 108139083 108139208 126 - 1.805 1.830 0.085
ENSG00000085491 E008 104.8923369 0.0003682738 1.052879e-02 3.004394e-02 1 108143543 108143710 168 - 1.891 1.890 -0.004
ENSG00000085491 E009 94.1298797 0.0002999690 2.424158e-04 1.143350e-03 1 108148279 108148386 108 - 1.853 1.785 -0.230
ENSG00000085491 E010 108.9062477 0.0002960362 7.944940e-06 5.399623e-05 1 108154983 108155084 102 - 1.919 1.825 -0.318
ENSG00000085491 E011 85.8220688 0.0003331004 4.118086e-06 2.985569e-05 1 108155085 108155135 51 - 1.821 1.685 -0.458
ENSG00000085491 E012 3.4205886 0.1495047227 2.373858e-01 3.711451e-01 1 108156701 108156885 185 - 0.463 0.788 1.436
ENSG00000085491 E013 139.7222691 0.0002984817 1.573619e-07 1.538829e-06 1 108157462 108157620 159 - 2.025 1.921 -0.349
ENSG00000085491 E014 122.3790840 0.0002703058 3.593247e-09 4.804699e-08 1 108161182 108161293 112 - 1.975 1.819 -0.524
ENSG00000085491 E015 96.4482896 0.0004846713 1.994250e-06 1.551687e-05 1 108181941 108182028 88 - 1.871 1.741 -0.439
ENSG00000085491 E016 99.5371704 0.0002897810 7.668812e-11 1.378661e-09 1 108185828 108185954 127 - 1.894 1.663 -0.784
ENSG00000085491 E017 0.1451727 0.0444259620 1.000000e+00   1 108192496 108192818 323 - 0.052 0.001 -6.556
ENSG00000085491 E018 9.2367675 0.0285124465 1.382999e-12 3.311069e-11 1 108198493 108199955 1463 - 0.517 1.511 3.778
ENSG00000085491 E019 95.8148237 0.0006852790 5.524377e-08 5.896539e-07 1 108199956 108201115 1160 - 1.872 1.693 -0.605