ENSG00000085433

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000369962 ENSG00000085433 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR47 protein_coding protein_coding 5.149396 1.753304 8.731952 0.1627872 0.08002945 2.309674 4.1615626 1.67243121 6.6964159 0.13899645 0.21923657 1.994994 0.8372583 0.95576667 0.76746667 -0.1883 1.835866e-03 8.602246e-06 FALSE TRUE
ENST00000369965 ENSG00000085433 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR47 protein_coding protein_coding 5.149396 1.753304 8.731952 0.1627872 0.08002945 2.309674 0.5726260 0.07326497 0.6596485 0.03680064 0.35951942 3.007622 0.1091625 0.04056667 0.07506667 0.0345 9.671403e-01 8.602246e-06 FALSE TRUE
MSTRG.1726.5 ENSG00000085433 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR47 protein_coding   5.149396 1.753304 8.731952 0.1627872 0.08002945 2.309674 0.2028749 0.00000000 0.9326432 0.00000000 0.07471127 6.558640 0.0281750 0.00000000 0.10680000 0.1068 8.602246e-06 8.602246e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000085433 E001 137.9030130 0.0008663824 7.778250e-28 1.085900e-25 1 108970214 108971322 1109 - 1.958 2.312 1.184
ENSG00000085433 E002 25.1803458 0.0008297986 3.136329e-02 7.461143e-02 1 108971323 108971337 15 - 1.294 1.465 0.592
ENSG00000085433 E003 52.7021057 0.0004921983 6.747649e-02 1.391568e-01 1 108971338 108971572 235 - 1.620 1.726 0.357
ENSG00000085433 E004 48.2988564 0.0004730122 2.066349e-02 5.287619e-02 1 108974536 108974754 219 - 1.578 1.714 0.462
ENSG00000085433 E005 38.5315391 0.0019257464 2.356255e-01 3.691090e-01 1 108981733 108981864 132 - 1.497 1.580 0.285
ENSG00000085433 E006 52.2922488 0.0004685988 1.786855e-01 2.994959e-01 1 108982609 108982779 171 - 1.624 1.703 0.269
ENSG00000085433 E007 52.8767539 0.0005772246 3.675449e-01 5.119195e-01 1 108983282 108983451 170 - 1.636 1.691 0.185
ENSG00000085433 E008 48.8783633 0.0018717888 5.045837e-01 6.404963e-01 1 108986523 108986680 158 - 1.622 1.581 -0.141
ENSG00000085433 E009 41.7199693 0.0078784392 5.245153e-01 6.580260e-01 1 108991254 108991329 76 - 1.557 1.504 -0.180
ENSG00000085433 E010 66.4472231 0.0004116484 2.896305e-03 9.959637e-03 1 108995580 108995837 258 - 1.775 1.611 -0.558
ENSG00000085433 E011 42.4243762 0.0005387227 9.297376e-02 1.797820e-01 1 109002224 109002402 179 - 1.577 1.465 -0.384
ENSG00000085433 E012 40.4807120 0.0030831633 2.771664e-01 4.166787e-01 1 109004592 109004715 124 - 1.551 1.474 -0.262
ENSG00000085433 E013 2.4420613 0.0077188528 5.239363e-01 6.575740e-01 1 109004716 109004718 3 - 0.500 0.375 -0.653
ENSG00000085433 E014 35.4418502 0.0035348226 6.580452e-01 7.672688e-01 1 109010916 109010991 76 - 1.486 1.454 -0.109
ENSG00000085433 E015 49.6573255 0.0004881662 1.452740e-02 3.942714e-02 1 109010992 109011179 188 - 1.650 1.494 -0.531
ENSG00000085433 E016 34.4402742 0.0006035365 1.339730e-02 3.683820e-02 1 109011180 109011345 166 - 1.499 1.306 -0.668
ENSG00000085433 E017 34.9655830 0.0107314807 5.686743e-03 1.778360e-02 1 109011346 109011526 181 - 1.519 1.257 -0.908
ENSG00000085433 E018 15.8323405 0.0165242118 2.205006e-02 5.578459e-02 1 109011527 109011545 19 - 1.199 0.894 -1.114
ENSG00000085433 E019 19.2847725 0.0010479766 1.774399e-02 4.658461e-02 1 109011546 109011575 30 - 1.273 1.026 -0.885
ENSG00000085433 E020 23.2988561 0.0008325272 8.885521e-04 3.574188e-03 1 109011576 109011642 67 - 1.360 1.026 -1.189
ENSG00000085433 E021 24.0910132 0.0010347538 2.153948e-02 5.472600e-02 1 109011643 109011718 76 - 1.360 1.147 -0.749
ENSG00000085433 E022 0.4355181 0.6405867309 1.000000e+00 1.000000e+00 1 109013820 109013840 21 - 0.164 0.000 -9.977
ENSG00000085433 E023 19.8487789 0.0016496232 1.359772e-02 3.730159e-02 1 109013841 109013849 9 - 1.283 1.026 -0.922
ENSG00000085433 E024 27.5131276 0.0006910461 1.942181e-02 5.024928e-02 1 109013850 109013925 76 - 1.410 1.207 -0.709
ENSG00000085433 E025 24.0621516 0.0046381540 7.060512e-01 8.042866e-01 1 109017518 109017601 84 - 1.324 1.291 -0.115
ENSG00000085433 E026 28.4132977 0.0007199586 6.902173e-04 2.863516e-03 1 109023355 109023521 167 - 1.435 1.126 -1.085
ENSG00000085433 E027 0.2965864 0.0645307932 1.893540e-02   1 109024865 109024992 128 - 0.000 0.373 13.420
ENSG00000085433 E028 0.0000000       1 109041520 109041547 28 -      
ENSG00000085433 E029 20.3719068 0.0175353040 7.754177e-03 2.316130e-02 1 109041862 109042113 252 - 1.303 0.965 -1.215