ENSG00000085415

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262124 ENSG00000085415 HEK293_OSMI2_2hA HEK293_TMG_2hB SEH1L protein_coding protein_coding 24.27173 12.36158 39.06522 3.116279 1.812737 1.659224 9.6214388 6.598268 15.8949523 1.175135 0.7182701 1.267132 0.4036042 0.55256667 0.40733333 -0.14523333 1.214129e-01 1.714486e-06 FALSE TRUE
ENST00000399892 ENSG00000085415 HEK293_OSMI2_2hA HEK293_TMG_2hB SEH1L protein_coding protein_coding 24.27173 12.36158 39.06522 3.116279 1.812737 1.659224 10.5693974 3.829410 17.5781026 1.088887 0.8230643 2.195643 0.4249042 0.30543333 0.44993333 0.14450000 1.714486e-06 1.714486e-06 FALSE TRUE
MSTRG.15529.12 ENSG00000085415 HEK293_OSMI2_2hA HEK293_TMG_2hB SEH1L protein_coding   24.27173 12.36158 39.06522 3.116279 1.812737 1.659224 0.8092033 1.166618 0.4765954 1.166618 0.3170272 -1.273852 0.0472250 0.06286667 0.01263333 -0.05023333 9.100911e-01 1.714486e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000085415 E001 0.0000000       18 12946963 12947132 170 +      
ENSG00000085415 E002 1.1854928 0.0189616855 2.712274e-01 4.099507e-01 18 12947133 12947626 494 + 0.375 0.187 -1.346
ENSG00000085415 E003 3.1169200 0.1647811377 6.281720e-01 7.437716e-01 18 12947995 12948010 16 + 0.618 0.501 -0.534
ENSG00000085415 E004 3.1169200 0.1647811377 6.281720e-01 7.437716e-01 18 12948011 12948011 1 + 0.618 0.501 -0.534
ENSG00000085415 E005 3.1169200 0.1647811377 6.281720e-01 7.437716e-01 18 12948012 12948013 2 + 0.618 0.501 -0.534
ENSG00000085415 E006 5.1076693 0.0244070442 3.631028e-01 5.075236e-01 18 12948014 12948024 11 + 0.785 0.680 -0.431
ENSG00000085415 E007 27.3529244 0.0358195623 2.841616e-01 4.243742e-01 18 12948025 12948035 11 + 1.447 1.337 -0.383
ENSG00000085415 E008 41.7213263 0.0103120189 5.182673e-02 1.124148e-01 18 12948036 12948047 12 + 1.629 1.510 -0.405
ENSG00000085415 E009 100.3930185 0.0180070872 2.502652e-02 6.192789e-02 18 12948048 12948138 91 + 2.012 1.859 -0.513
ENSG00000085415 E010 142.5023499 0.0238851959 4.569165e-02 1.014658e-01 18 12948139 12948232 94 + 2.162 2.012 -0.502
ENSG00000085415 E011 4.4329349 0.0037586288 9.441744e-01 9.690093e-01 18 12948505 12948786 282 + 0.692 0.726 0.142
ENSG00000085415 E012 2.4022307 0.0063055179 4.952515e-01 6.320858e-01 18 12949818 12949907 90 + 0.471 0.627 0.724
ENSG00000085415 E013 138.1476003 0.0043296740 2.241500e-04 1.066922e-03 18 12951855 12951905 51 + 2.146 2.015 -0.439
ENSG00000085415 E014 195.6651595 0.0032056749 1.126114e-07 1.133274e-06 18 12955463 12955609 147 + 2.305 2.143 -0.540
ENSG00000085415 E015 0.7405235 0.0157918025 6.192466e-01 7.367144e-01 18 12955610 12955610 1 + 0.201 0.318 0.876
ENSG00000085415 E016 234.3017701 0.0002852181 1.001405e-05 6.654032e-05 18 12963160 12963371 212 + 2.359 2.301 -0.191
ENSG00000085415 E017 2.4002681 0.1791412352 6.673779e-01 7.746367e-01 18 12964436 12964495 60 + 0.471 0.615 0.674
ENSG00000085415 E018 191.0305343 0.0002463957 4.190478e-02 9.455152e-02 18 12971153 12971251 99 + 2.254 2.254 0.001
ENSG00000085415 E019 0.7468662 0.0235518918 6.257920e-01 7.418553e-01 18 12971252 12971252 1 + 0.201 0.318 0.874
ENSG00000085415 E020 1.9251877 0.0074783774 5.866488e-03 1.826228e-02 18 12971563 12974555 2993 + 0.550 0.000 -11.295
ENSG00000085415 E021 3.1838299 0.0062150079 3.575361e-01 5.019277e-01 18 12975733 12975882 150 + 0.617 0.500 -0.535
ENSG00000085415 E022 7.4566185 0.0533886602 2.712073e-01 4.099246e-01 18 12976288 12978751 2464 + 0.937 0.771 -0.642
ENSG00000085415 E023 176.5196061 0.0010228555 1.346635e-03 5.132086e-03 18 12978752 12978828 77 + 2.233 2.183 -0.167
ENSG00000085415 E024 163.5619949 0.0020638866 1.030702e-03 4.064348e-03 18 12978829 12978892 64 + 2.204 2.131 -0.245
ENSG00000085415 E025 249.7051120 0.0040763893 3.496482e-01 4.938570e-01 18 12982518 12982675 158 + 2.362 2.372 0.031
ENSG00000085415 E026 3.9055433 0.0202780785 1.628557e-03 6.052483e-03 18 12982676 12983217 542 + 0.771 0.188 -3.178
ENSG00000085415 E027 228.5622147 0.0002843496 2.565000e-01 3.934636e-01 18 12984040 12984190 151 + 2.303 2.386 0.279
ENSG00000085415 E028 547.5493034 0.0029088209 1.853565e-07 1.787307e-06 18 12985240 12986861 1622 + 2.640 2.831 0.636
ENSG00000085415 E029 368.9652296 0.0204388150 2.823161e-04 1.307804e-03 18 12986862 12987536 675 + 2.410 2.729 1.063