ENSG00000085365

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000506858 ENSG00000085365 HEK293_OSMI2_2hA HEK293_TMG_2hB SCAMP1 protein_coding processed_transcript 13.88366 2.033397 23.8806 0.3146286 0.7613433 3.547402 1.10026628 0.2238553 1.9679336 0.12791555 0.1629112 3.08030613 0.07315000 0.09426667 0.08226667 -0.01200000 0.93090799 0.02687744 FALSE FALSE
ENST00000510030 ENSG00000085365 HEK293_OSMI2_2hA HEK293_TMG_2hB SCAMP1 protein_coding protein_coding 13.88366 2.033397 23.8806 0.3146286 0.7613433 3.547402 0.05595354 0.1027202 0.1069857 0.05168937 0.1069857 0.05358644 0.02247083 0.05570000 0.00440000 -0.05130000 0.24490850 0.02687744   FALSE
ENST00000614488 ENSG00000085365 HEK293_OSMI2_2hA HEK293_TMG_2hB SCAMP1 protein_coding nonsense_mediated_decay 13.88366 2.033397 23.8806 0.3146286 0.7613433 3.547402 0.94912407 0.1788861 1.3199076 0.08990761 0.1472654 2.81573746 0.05832500 0.10220000 0.05510000 -0.04710000 0.83670728 0.02687744 TRUE TRUE
ENST00000618166 ENSG00000085365 HEK293_OSMI2_2hA HEK293_TMG_2hB SCAMP1 protein_coding protein_coding 13.88366 2.033397 23.8806 0.3146286 0.7613433 3.547402 0.43897270 0.1367648 0.6157126 0.07775734 0.3016797 2.09199471 0.02637083 0.08183333 0.02660000 -0.05523333 0.72611579 0.02687744 FALSE TRUE
ENST00000621999 ENSG00000085365 HEK293_OSMI2_2hA HEK293_TMG_2hB SCAMP1 protein_coding protein_coding 13.88366 2.033397 23.8806 0.3146286 0.7613433 3.547402 2.76156496 1.0153273 5.3426134 0.29572785 0.4706500 2.38415895 0.38933750 0.51316667 0.22290000 -0.29026667 0.11568285 0.02687744 FALSE TRUE
MSTRG.26453.3 ENSG00000085365 HEK293_OSMI2_2hA HEK293_TMG_2hB SCAMP1 protein_coding   13.88366 2.033397 23.8806 0.3146286 0.7613433 3.547402 7.73655743 0.1938453 13.4587542 0.19384531 0.3960106 6.04599782 0.37088750 0.07813333 0.56366667 0.48553333 0.02687744 0.02687744 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000085365 E001 10.6738782 0.0015876891 7.361316e-01 8.267125e-01 5 78360611 78360616 6 + 0.930 0.909 -0.082
ENSG00000085365 E002 13.1839093 0.0012959433 3.041965e-01 4.462082e-01 5 78360617 78360627 11 + 1.023 0.909 -0.426
ENSG00000085365 E003 54.3926085 0.0006780789 3.944148e-01 5.382483e-01 5 78360628 78360728 101 + 1.593 1.562 -0.105
ENSG00000085365 E004 0.9514719 0.1924101947 1.783286e-03 6.546043e-03 5 78360805 78361056 252 + 0.049 0.667 4.935
ENSG00000085365 E005 1.6984293 0.0082096134 7.375611e-01 8.276984e-01 5 78370620 78370805 186 + 0.341 0.276 -0.425
ENSG00000085365 E006 72.6670496 0.0003518581 1.243746e-04 6.339258e-04 5 78388837 78388914 78 + 1.736 1.506 -0.782
ENSG00000085365 E007 93.5856891 0.0003961947 1.260479e-03 4.844968e-03 5 78415520 78415618 99 + 1.837 1.682 -0.523
ENSG00000085365 E008 79.6961285 0.0003326645 9.569406e-03 2.770740e-02 5 78416541 78416602 62 + 1.767 1.640 -0.432
ENSG00000085365 E009 72.4352732 0.0003790667 1.373685e-03 5.220891e-03 5 78416603 78416649 47 + 1.732 1.552 -0.614
ENSG00000085365 E010 92.1368077 0.0007379190 3.286724e-06 2.439604e-05 5 78418775 78418903 129 + 1.841 1.583 -0.875
ENSG00000085365 E011 89.1204805 0.0003347575 9.609202e-06 6.414079e-05 5 78421801 78421960 160 + 1.825 1.583 -0.821
ENSG00000085365 E012 63.0826199 0.0004270914 1.541385e-03 5.769974e-03 5 78449933 78450034 102 + 1.676 1.481 -0.664
ENSG00000085365 E013 8.0755695 0.0021209651 7.803879e-01 8.588632e-01 5 78457793 78457951 159 + 0.820 0.801 -0.074
ENSG00000085365 E014 80.3552584 0.0003278475 5.494235e-02 1.178927e-01 5 78459245 78459362 118 + 1.765 1.682 -0.284
ENSG00000085365 E015 83.3018331 0.0003299960 1.765152e-01 2.967168e-01 5 78475504 78475654 151 + 1.775 1.727 -0.161
ENSG00000085365 E016 692.1460026 0.0007653297 1.125684e-28 1.685830e-26 5 78475655 78480739 5085 + 2.653 2.839 0.621
ENSG00000085365 E017 0.6717251 0.0255644679 4.716995e-01 6.107721e-01 5 78481045 78481199 155 + 0.133 0.276 1.313