ENSG00000084463

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000261167 ENSG00000084463 HEK293_OSMI2_2hA HEK293_TMG_2hB WBP11 protein_coding protein_coding 79.85046 81.17158 89.78772 3.487101 1.253958 0.1455264 20.850783 29.37308133 16.56813 1.43549329 0.6765131 -0.8257047 0.2643958 0.3622 0.1845333 -0.17766667 1.560312e-13 2.448605e-17 FALSE TRUE
ENST00000544764 ENSG00000084463 HEK293_OSMI2_2hA HEK293_TMG_2hB WBP11 protein_coding retained_intron 79.85046 81.17158 89.78772 3.487101 1.253958 0.1455264 3.125843 0.04022161 10.36970 0.04022161 1.7889122 7.6912411 0.0361375 0.0005 0.1150333 0.11453333 2.448605e-17 2.448605e-17   FALSE
MSTRG.6886.2 ENSG00000084463 HEK293_OSMI2_2hA HEK293_TMG_2hB WBP11 protein_coding   79.85046 81.17158 89.78772 3.487101 1.253958 0.1455264 55.456425 51.62876825 62.63116 2.74664329 0.7477952 0.2786564 0.6944125 0.6358 0.6980333 0.06223333 1.960333e-01 2.448605e-17 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000084463 E001 1487.6780720 3.493395e-03 1.930402e-17 9.101764e-16 12 14784582 14787062 2481 - 2.998 3.252 0.843
ENSG00000084463 E002 1481.3705814 4.324844e-04 1.007076e-12 2.471342e-11 12 14787063 14787498 436 - 3.097 3.206 0.362
ENSG00000084463 E003 587.5048184 1.485956e-03 1.675244e-02 4.439224e-02 12 14788951 14789133 183 - 2.718 2.791 0.245
ENSG00000084463 E004 904.5812358 1.022421e-03 4.709440e-06 3.372479e-05 12 14790456 14790688 233 - 2.886 2.990 0.347
ENSG00000084463 E005 611.3865468 1.243191e-04 1.476268e-03 5.560351e-03 12 14790689 14790749 61 - 2.740 2.807 0.222
ENSG00000084463 E006 772.9286849 1.443845e-04 2.266885e-01 3.584433e-01 12 14791169 14791270 102 - 2.880 2.882 0.008
ENSG00000084463 E007 1042.7674275 9.384837e-05 8.168934e-11 1.463156e-09 12 14793731 14793922 192 - 3.044 2.990 -0.181
ENSG00000084463 E008 946.8573798 2.937142e-04 9.976017e-11 1.760364e-09 12 14794537 14794736 200 - 3.010 2.941 -0.227
ENSG00000084463 E009 637.4858784 1.341281e-04 7.390250e-04 3.039274e-03 12 14794971 14795104 134 - 2.817 2.787 -0.101
ENSG00000084463 E010 785.4387706 1.447110e-03 3.648453e-04 1.638043e-03 12 14796807 14797003 197 - 2.922 2.863 -0.198
ENSG00000084463 E011 0.4481018 8.712094e-02 7.375045e-01 8.276447e-01 12 14799441 14799634 194 - 0.133 0.200 0.710
ENSG00000084463 E012 400.3229986 1.961671e-03 4.848557e-05 2.741024e-04 12 14799635 14799665 31 - 2.649 2.556 -0.311
ENSG00000084463 E013 449.1601137 1.355141e-03 5.363639e-10 8.359043e-09 12 14799666 14799728 63 - 2.719 2.588 -0.437
ENSG00000084463 E014 404.2033459 3.457693e-03 4.599997e-05 2.615021e-04 12 14800752 14800783 32 - 2.666 2.548 -0.394
ENSG00000084463 E015 1.7714040 2.411186e-01 5.813606e-01 7.058993e-01 12 14801000 14801319 320 - 0.499 0.394 -0.545
ENSG00000084463 E016 503.0525090 4.822300e-03 2.138728e-04 1.023558e-03 12 14801320 14801428 109 - 2.761 2.643 -0.393
ENSG00000084463 E017 0.5964967 2.272009e-02 2.173881e-01 3.472385e-01 12 14801878 14802032 155 - 0.317 0.112 -1.861
ENSG00000084463 E018 189.0551050 6.091766e-03 1.811209e-03 6.634468e-03 12 14803352 14803353 2 - 2.341 2.216 -0.419
ENSG00000084463 E019 326.2908470 6.998797e-03 5.228389e-05 2.931869e-04 12 14803354 14803498 145 - 2.597 2.433 -0.546