ENSG00000083520

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000377767 ENSG00000083520 HEK293_OSMI2_2hA HEK293_TMG_2hB DIS3 protein_coding protein_coding 42.24985 7.304061 74.99953 0.5330773 2.376973 3.358329 2.024291 0.8270025 4.055714 0.05689523 0.101152 2.280205 0.07219583 0.1148000 0.05416667 -0.06063333 0.0004768629 0.0004768629 FALSE TRUE
ENST00000377780 ENSG00000083520 HEK293_OSMI2_2hA HEK293_TMG_2hB DIS3 protein_coding protein_coding 42.24985 7.304061 74.99953 0.5330773 2.376973 3.358329 9.751368 3.7528503 14.466325 0.41775551 1.013818 1.943798 0.33963333 0.5249667 0.19243333 -0.33253333 0.0023949575 0.0004768629 FALSE TRUE
MSTRG.8876.1 ENSG00000083520 HEK293_OSMI2_2hA HEK293_TMG_2hB DIS3 protein_coding   42.24985 7.304061 74.99953 0.5330773 2.376973 3.358329 29.665113 2.6293614 55.659277 0.83547049 1.167057 4.398621 0.57283750 0.3483000 0.74263333 0.39433333 0.0070271671 0.0004768629 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000083520 E001 0.1817044 0.0411069381 4.480635e-02   13 72744481 72744531 51 - 0.000 0.288 11.877
ENSG00000083520 E002 244.1755349 0.0021458262 3.697487e-12 8.261326e-11 13 72752169 72756678 4510 - 2.160 2.465 1.017
ENSG00000083520 E003 52.7172796 0.0010501503 1.220196e-03 4.709050e-03 13 72756679 72756935 257 - 1.512 1.774 0.890
ENSG00000083520 E004 53.4977422 0.0004389577 5.724737e-05 3.178495e-04 13 72756936 72757442 507 - 1.512 1.813 1.023
ENSG00000083520 E005 115.2907930 0.0002449849 2.741574e-01 4.132838e-01 13 72757443 72758906 1464 - 1.889 1.888 -0.002
ENSG00000083520 E006 7.9681867 0.0279375820 7.018556e-01 8.008383e-01 13 72758907 72758908 2 - 0.775 0.884 0.424
ENSG00000083520 E007 53.5639477 0.0062923505 1.799554e-06 1.414019e-05 13 72758909 72759043 135 - 1.495 1.880 1.306
ENSG00000083520 E008 203.3904685 0.0225354649 6.701964e-08 7.039626e-07 13 72759044 72759163 120 - 2.034 2.523 1.636
ENSG00000083520 E009 470.5090813 0.0146688203 8.657023e-12 1.821465e-10 13 72759164 72759472 309 - 2.402 2.869 1.556
ENSG00000083520 E010 328.3589996 0.0039307364 5.530974e-18 2.792714e-16 13 72759473 72759674 202 - 2.270 2.651 1.271
ENSG00000083520 E011 316.8353348 0.0001652137 8.897291e-16 3.385349e-14 13 72759675 72759878 204 - 2.281 2.546 0.884
ENSG00000083520 E012 228.7013209 0.0005284463 5.825115e-05 3.228455e-04 13 72760529 72760651 123 - 2.154 2.342 0.627
ENSG00000083520 E013 260.8362503 0.0002197815 3.053194e-06 2.280290e-05 13 72761363 72761521 159 - 2.209 2.404 0.651
ENSG00000083520 E014 358.1817581 0.0001410318 5.298059e-01 6.625311e-01 13 72761646 72761814 169 - 2.368 2.439 0.238
ENSG00000083520 E015 439.3501251 0.0002243081 9.196519e-02 1.781754e-01 13 72761923 72762137 215 - 2.464 2.475 0.036
ENSG00000083520 E016 339.5766928 0.0001433635 7.493921e-01 8.363508e-01 13 72763451 72763607 157 - 2.345 2.409 0.211
ENSG00000083520 E017 275.2638335 0.0004233346 6.829734e-01 7.863027e-01 13 72765972 72766058 87 - 2.258 2.298 0.136
ENSG00000083520 E018 335.9295261 0.0001582973 7.382109e-02 1.496286e-01 13 72768785 72768912 128 - 2.349 2.351 0.006
ENSG00000083520 E019 307.0825639 0.0001691046 1.553526e-02 4.170328e-02 13 72770904 72770988 85 - 2.313 2.292 -0.070
ENSG00000083520 E020 240.7342231 0.0001719703 7.953163e-02 1.587959e-01 13 72771081 72771145 65 - 2.206 2.199 -0.023
ENSG00000083520 E021 310.8261779 0.0011531601 1.270098e-03 4.877390e-03 13 72771795 72771896 102 - 2.323 2.264 -0.197
ENSG00000083520 E022 393.5930920 0.0001297340 4.946392e-14 1.468776e-12 13 72772159 72772275 117 - 2.437 2.266 -0.568
ENSG00000083520 E023 431.2112474 0.0001332816 1.759275e-14 5.592103e-13 13 72772693 72772839 147 - 2.476 2.312 -0.545
ENSG00000083520 E024 377.8041608 0.0001367285 4.126484e-18 2.107711e-16 13 72773684 72773821 138 - 2.422 2.204 -0.727
ENSG00000083520 E025 315.4399795 0.0001515288 2.367941e-14 7.367373e-13 13 72773946 72774059 114 - 2.344 2.138 -0.688
ENSG00000083520 E026 379.3721847 0.0171811740 2.189443e-04 1.045005e-03 13 72775211 72775375 165 - 2.424 2.220 -0.679
ENSG00000083520 E027 343.8750473 0.0003105257 1.013677e-20 7.094131e-19 13 72775925 72776092 168 - 2.386 2.117 -0.899
ENSG00000083520 E028 243.0848848 0.0002116791 1.908674e-14 6.031569e-13 13 72777420 72777493 74 - 2.236 1.983 -0.847
ENSG00000083520 E029 368.6675990 0.0009577558 5.734300e-17 2.538191e-15 13 72778187 72778380 194 - 2.415 2.173 -0.808
ENSG00000083520 E030 233.0612549 0.0007279663 1.095881e-15 4.114375e-14 13 72780846 72781003 158 - 2.220 1.924 -0.994
ENSG00000083520 E031 18.3729773 0.0012645781 9.456022e-02 1.822266e-01 13 72781289 72781356 68 - 1.141 0.976 -0.598
ENSG00000083520 E032 198.3628558 0.0005990027 1.880729e-12 4.406361e-11 13 72781605 72782096 492 - 2.148 1.878 -0.907