ENSG00000083223

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000375957 ENSG00000083223 HEK293_OSMI2_2hA HEK293_TMG_2hB TUT7 protein_coding protein_coding 4.842192 1.558021 7.308808 0.06103906 0.1788381 2.222664 0.3809746 0.9148561 0.05487563 0.07270512 0.05487563 -3.833480 0.2229000 0.5866667 0.007166667 -0.5795 1.542982e-08 1.542982e-08 FALSE TRUE
ENST00000375963 ENSG00000083223 HEK293_OSMI2_2hA HEK293_TMG_2hB TUT7 protein_coding protein_coding 4.842192 1.558021 7.308808 0.06103906 0.1788381 2.222664 2.3136610 0.3379796 3.93709954 0.05281242 0.38049984 3.503718 0.4247833 0.2171333 0.537733333 0.3206 6.489729e-04 1.542982e-08 FALSE TRUE
MSTRG.32929.5 ENSG00000083223 HEK293_OSMI2_2hA HEK293_TMG_2hB TUT7 protein_coding   4.842192 1.558021 7.308808 0.06103906 0.1788381 2.222664 1.5086114 0.2590500 2.96068500 0.03097806 0.14532058 3.464849 0.2500167 0.1654667 0.404866667 0.2394 1.087403e-04 1.542982e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000083223 E001 0.1482932 0.0427927613 5.193391e-02   9 86287733 86287734 2 - 0.000 0.273 11.723
ENSG00000083223 E002 51.9218002 0.0255331908 4.001014e-11 7.542842e-10 9 86287735 86288402 668 - 1.413 2.030 2.093
ENSG00000083223 E003 17.7944333 0.0074931409 8.220413e-07 6.952688e-06 9 86288403 86288561 159 - 1.007 1.514 1.787
ENSG00000083223 E004 28.0091805 0.0048668654 2.233324e-06 1.719276e-05 9 86288562 86288744 183 - 1.219 1.636 1.439
ENSG00000083223 E005 12.2442099 0.0015488707 2.468827e-01 3.822893e-01 9 86298767 86298830 64 - 0.952 1.123 0.626
ENSG00000083223 E006 60.7366826 0.0018620338 3.388540e-02 7.952586e-02 9 86301276 86301601 326 - 1.608 1.771 0.552
ENSG00000083223 E007 31.7331746 0.0011462257 3.581416e-02 8.322212e-02 9 86303086 86303201 116 - 1.325 1.525 0.690
ENSG00000083223 E008 24.8026515 0.0020835498 3.317036e-03 1.119748e-02 9 86304856 86304947 92 - 1.203 1.490 0.999
ENSG00000083223 E009 19.4283697 0.0178776214 6.033302e-03 1.869893e-02 9 86305192 86305239 48 - 1.095 1.425 1.164
ENSG00000083223 E010 34.7771408 0.0076418309 1.369035e-03 5.205618e-03 9 86308429 86308606 178 - 1.344 1.637 1.004
ENSG00000083223 E011 18.4594206 0.0022733679 2.971183e-02 7.139181e-02 9 86309212 86309289 78 - 1.095 1.344 0.880
ENSG00000083223 E012 25.9050978 0.0007930560 3.547131e-01 4.990370e-01 9 86309463 86309576 114 - 1.261 1.377 0.404
ENSG00000083223 E013 31.4607006 0.0006371088 4.344029e-01 5.767854e-01 9 86309928 86310017 90 - 1.363 1.326 -0.128
ENSG00000083223 E014 34.2838781 0.0006008730 3.994784e-01 5.432254e-01 9 86310706 86310809 104 - 1.400 1.361 -0.136
ENSG00000083223 E015 24.2542357 0.0007867437 4.654874e-03 1.497151e-02 9 86317219 86317276 58 - 1.286 0.990 -1.063
ENSG00000083223 E016 24.8067332 0.0007671043 3.085472e-01 4.508406e-01 9 86318958 86319058 101 - 1.273 1.202 -0.249
ENSG00000083223 E017 20.6048284 0.0009507869 9.024228e-01 9.418524e-01 9 86319584 86319670 87 - 1.183 1.202 0.068
ENSG00000083223 E018 28.0414062 0.0106595979 8.930276e-01 9.356335e-01 9 86322325 86322475 151 - 1.310 1.327 0.057
ENSG00000083223 E019 21.2418080 0.0008878202 4.375309e-01 5.794928e-01 9 86322873 86322953 81 - 1.203 1.151 -0.184
ENSG00000083223 E020 96.9590940 0.0002917091 5.007718e-04 2.163062e-03 9 86322954 86323627 674 - 1.855 1.700 -0.524
ENSG00000083223 E021 43.7262172 0.0005282215 7.171080e-04 2.960297e-03 9 86323628 86323960 333 - 1.528 1.269 -0.897
ENSG00000083223 E022 0.4439371 0.0215781394 1.000000e+00 1.000000e+00 9 86324362 86324653 292 - 0.134 0.000 -12.786
ENSG00000083223 E023 24.4572069 0.0044055908 2.945684e-02 7.088972e-02 9 86325334 86325514 181 - 1.281 1.062 -0.779
ENSG00000083223 E024 0.8856842 0.0807978147 4.761739e-01 6.148342e-01 9 86326371 86326491 121 - 0.236 0.000 -13.542
ENSG00000083223 E025 21.9453281 0.0418302522 7.471829e-02 1.510850e-01 9 86328340 86328492 153 - 1.238 0.996 -0.872
ENSG00000083223 E026 0.4772466 0.0222367816 3.042133e-02 7.278793e-02 9 86336879 86337418 540 - 0.049 0.440 3.862
ENSG00000083223 E027 22.4102534 0.0115849152 1.596233e-01 2.746139e-01 9 86337419 86337538 120 - 1.235 1.096 -0.496
ENSG00000083223 E028 24.4826865 0.0007759213 1.424595e-01 2.513811e-01 9 86338823 86338949 127 - 1.273 1.151 -0.429
ENSG00000083223 E029 21.8624201 0.0355922358 5.605630e-02 1.197976e-01 9 86340036 86340105 70 - 1.243 0.986 -0.926
ENSG00000083223 E030 21.3091139 0.0061212799 5.177982e-03 1.641213e-02 9 86341002 86341053 52 - 1.238 0.904 -1.214
ENSG00000083223 E031 24.7112831 0.0011654023 8.909686e-03 2.605649e-02 9 86343075 86343163 89 - 1.292 1.028 -0.944
ENSG00000083223 E032 0.1482932 0.0427927613 5.193391e-02   9 86344870 86344976 107 - 0.000 0.273 14.425
ENSG00000083223 E033 35.5773266 0.0005741483 1.997977e-03 7.219492e-03 9 86344977 86345154 178 - 1.440 1.177 -0.918
ENSG00000083223 E034 28.6893011 0.0011919447 1.275766e-04 6.481018e-04 9 86345669 86345785 117 - 1.361 0.950 -1.474
ENSG00000083223 E035 34.9915954 0.0068219557 7.161109e-06 4.919143e-05 9 86346299 86346480 182 - 1.450 0.950 -1.781
ENSG00000083223 E036 64.4290153 0.0003910027 5.810813e-05 3.221728e-04 9 86352680 86353230 551 - 1.689 1.437 -0.863
ENSG00000083223 E037 16.6814704 0.0010427121 2.233595e-02 5.637398e-02 9 86354271 86354488 218 - 1.132 0.854 -1.030