ENSG00000082458

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000374355 ENSG00000082458 HEK293_OSMI2_2hA HEK293_TMG_2hB DLG3 protein_coding protein_coding 6.77464 5.98625 7.897234 0.8462309 0.5099234 0.3991127 1.4492753 1.165819 1.957395 0.09355902 0.15768096 0.7426208 0.2125917 0.2043333 0.2479 0.04356667 6.646655e-01 6.876727e-11 FALSE TRUE
ENST00000461646 ENSG00000082458 HEK293_OSMI2_2hA HEK293_TMG_2hB DLG3 protein_coding processed_transcript 6.77464 5.98625 7.897234 0.8462309 0.5099234 0.3991127 0.3902584 1.280137 0.000000 0.75069771 0.00000000 -7.0113810 0.0653250 0.1967333 0.0000 -0.19673333 1.216122e-01 6.876727e-11 FALSE TRUE
ENST00000489733 ENSG00000082458 HEK293_OSMI2_2hA HEK293_TMG_2hB DLG3 protein_coding processed_transcript 6.77464 5.98625 7.897234 0.8462309 0.5099234 0.3991127 1.0378977 0.000000 1.537585 0.00000000 0.39437309 7.2738746 0.1297125 0.0000000 0.1901 0.19010000 6.876727e-11 6.876727e-11 FALSE FALSE
ENST00000542398 ENSG00000082458 HEK293_OSMI2_2hA HEK293_TMG_2hB DLG3 protein_coding protein_coding 6.77464 5.98625 7.897234 0.8462309 0.5099234 0.3991127 3.1966903 3.111083 3.582680 0.48963860 0.03985233 0.2030138 0.4905000 0.5230333 0.4568 -0.06623333 7.255926e-01 6.876727e-11 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000082458 E001 0.0000000       X 70444835 70444860 26 +      
ENSG00000082458 E002 0.1472490 0.0437728274 5.181171e-01   X 70444861 70445135 275 + 0.115 0.000 -10.438
ENSG00000082458 E003 0.0000000       X 70445136 70445558 423 +      
ENSG00000082458 E004 0.0000000       X 70448580 70448633 54 +      
ENSG00000082458 E005 0.0000000       X 70448913 70448963 51 +      
ENSG00000082458 E006 0.1451727 0.0429797684 5.191585e-01   X 70449359 70449483 125 + 0.115 0.000 -12.267
ENSG00000082458 E007 0.2903454 0.2778515887 2.913084e-01   X 70449690 70449859 170 + 0.205 0.000 -12.545
ENSG00000082458 E008 0.1451727 0.0429797684 5.191585e-01   X 70450169 70450305 137 + 0.115 0.000 -12.267
ENSG00000082458 E009 0.0000000       X 70450306 70450638 333 +      
ENSG00000082458 E010 0.2903454 0.2778515887 2.913084e-01   X 70450639 70450783 145 + 0.205 0.000 -12.545
ENSG00000082458 E011 0.2903454 0.2778515887 2.913084e-01   X 70451867 70452026 160 + 0.205 0.000 -12.545
ENSG00000082458 E012 14.8641895 0.0012203469 1.642421e-03 6.096710e-03 X 70452305 70452755 451 + 1.302 1.074 -0.809
ENSG00000082458 E013 16.5512902 0.0033385861 3.577213e-02 8.314222e-02 X 70453637 70453793 157 + 1.308 1.176 -0.465
ENSG00000082458 E014 13.7933062 0.0042713013 5.662654e-01 6.932744e-01 X 70454214 70454316 103 + 1.117 1.215 0.351
ENSG00000082458 E015 1.0997650 0.0189293487 8.460390e-02 1.667633e-01 X 70455096 70455102 7 + 0.115 0.440 2.539
ENSG00000082458 E016 1.2470141 0.0135012560 2.360963e-01 3.696449e-01 X 70455103 70455106 4 + 0.205 0.440 1.538
ENSG00000082458 E017 16.8705678 0.0011943026 1.128605e-02 3.184457e-02 X 70455107 70455263 157 + 1.333 1.176 -0.553
ENSG00000082458 E018 1.2502232 0.2260487279 6.956788e-01 7.962179e-01 X 70455787 70455788 2 + 0.280 0.388 0.674
ENSG00000082458 E019 6.0644637 0.0028193170 1.290436e-01 2.328477e-01 X 70455789 70456102 314 + 0.917 0.771 -0.564
ENSG00000082458 E020 0.5890081 0.0194413712 9.847211e-01 9.945118e-01 X 70479118 70479149 32 + 0.205 0.230 0.211
ENSG00000082458 E021 41.1950647 0.0005755967 2.331498e-03 8.248703e-03 X 70479150 70479264 115 + 1.685 1.573 -0.381
ENSG00000082458 E022 53.9352560 0.0006012515 2.043778e-03 7.361679e-03 X 70492107 70492283 177 + 1.791 1.696 -0.319
ENSG00000082458 E023 42.5206045 0.0006884837 1.286847e-03 4.933108e-03 X 70492521 70492596 76 + 1.699 1.577 -0.412
ENSG00000082458 E024 42.3932835 0.0008540016 1.609624e-02 4.295150e-02 X 70493376 70493475 100 + 1.680 1.601 -0.268
ENSG00000082458 E025 0.4782907 0.0217681645 8.065643e-02 1.605780e-01 X 70495408 70495453 46 + 0.000 0.312 13.416
ENSG00000082458 E026 0.0000000       X 70497165 70497179 15 +      
ENSG00000082458 E027 27.8937787 0.0089490368 6.219412e-02 1.303401e-01 X 70497180 70497221 42 + 1.514 1.411 -0.354
ENSG00000082458 E028 34.2464596 0.0030190320 3.217525e-02 7.619367e-02 X 70498520 70498570 51 + 1.595 1.511 -0.287
ENSG00000082458 E029 50.6103939 0.0004639147 1.556162e-03 5.817435e-03 X 70499176 70499277 102 + 1.766 1.665 -0.345
ENSG00000082458 E030 49.0057762 0.0005155240 6.465564e-02 1.344694e-01 X 70499877 70499964 88 + 1.724 1.684 -0.136
ENSG00000082458 E031 50.8388219 0.0005075201 1.524840e-02 4.104932e-02 X 70499965 70500049 85 + 1.755 1.690 -0.221
ENSG00000082458 E032 50.1188792 0.0007062700 2.486383e-01 3.843198e-01 X 70500471 70500580 110 + 1.719 1.714 -0.017
ENSG00000082458 E033 0.1472490 0.0437728274 5.181171e-01   X 70500581 70500897 317 + 0.115 0.000 -12.261
ENSG00000082458 E034 46.5361746 0.0005393243 5.685728e-01 6.952188e-01 X 70500898 70500989 92 + 1.671 1.693 0.074
ENSG00000082458 E035 505.4190943 0.0026282009 1.280561e-14 4.149818e-13 X 70502163 70505490 3328 + 2.604 2.789 0.616