ENSG00000082269

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000194672 ENSG00000082269 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM135A protein_coding nonsense_mediated_decay 7.588388 3.911843 11.91133 0.3521089 0.4572793 1.603942 0.4397096 0.1207191 0.88877565 0.06219538 0.47021488 2.781491 0.06258333 0.02966667 0.07190000 0.04223333 8.938765e-01 1.810414e-08 FALSE TRUE
ENST00000361499 ENSG00000082269 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM135A protein_coding protein_coding 7.588388 3.911843 11.91133 0.3521089 0.4572793 1.603942 0.6478653 0.1372900 1.31308741 0.13728997 0.20641237 3.167177 0.06143750 0.03806667 0.10990000 0.07183333 2.494162e-01 1.810414e-08 FALSE TRUE
ENST00000418814 ENSG00000082269 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM135A protein_coding protein_coding 7.588388 3.911843 11.91133 0.3521089 0.4572793 1.603942 0.5807101 0.0000000 0.88917151 0.00000000 0.51297975 6.490524 0.05516250 0.00000000 0.07503333 0.07503333 3.514880e-01 1.810414e-08 FALSE TRUE
ENST00000505675 ENSG00000082269 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM135A protein_coding retained_intron 7.588388 3.911843 11.91133 0.3521089 0.4572793 1.603942 0.3544522 1.0551552 0.01373264 0.59790122 0.01373264 -5.488047 0.09721667 0.24816667 0.00110000 -0.24706667 1.661963e-05 1.810414e-08   FALSE
ENST00000515280 ENSG00000082269 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM135A protein_coding protein_coding 7.588388 3.911843 11.91133 0.3521089 0.4572793 1.603942 0.3043409 0.0000000 0.89193750 0.00000000 0.12512887 6.494956 0.02673333 0.00000000 0.07483333 0.07483333 5.211331e-07 1.810414e-08 TRUE TRUE
MSTRG.28487.10 ENSG00000082269 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM135A protein_coding   7.588388 3.911843 11.91133 0.3521089 0.4572793 1.603942 1.3668862 1.7954205 1.15080686 0.20412127 0.30696416 -0.637207 0.28932917 0.47083333 0.09746667 -0.37336667 6.014917e-04 1.810414e-08   FALSE
MSTRG.28487.5 ENSG00000082269 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM135A protein_coding   7.588388 3.911843 11.91133 0.3521089 0.4572793 1.603942 1.9249344 0.3719456 4.00626847 0.05282473 0.32303746 3.394417 0.18810833 0.09720000 0.33550000 0.23830000 1.810414e-08 1.810414e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000082269 E001 0.0000000       6 70412941 70413090 150 +      
ENSG00000082269 E002 0.0000000       6 70413421 70413424 4 +      
ENSG00000082269 E003 0.4418608 0.0241839675 1.000000e+00 1.000000e+00 6 70413425 70413495 71 + 0.118 0.000 -8.749
ENSG00000082269 E004 0.4418608 0.0241839675 1.000000e+00 1.000000e+00 6 70413496 70413496 1 + 0.118 0.000 -10.951
ENSG00000082269 E005 0.8900644 0.0337891957 5.818473e-01 7.063305e-01 6 70413497 70413507 11 + 0.211 0.000 -11.793
ENSG00000082269 E006 1.0415798 0.0843955190 4.975636e-01 6.341072e-01 6 70413508 70413513 6 + 0.238 0.000 -11.775
ENSG00000082269 E007 1.1888288 0.0110612160 3.783108e-01 5.225906e-01 6 70413514 70413514 1 + 0.263 0.000 -12.267
ENSG00000082269 E008 7.6274954 0.0022124848 1.856898e-01 3.083604e-01 6 70413515 70413543 29 + 0.778 0.608 -0.708
ENSG00000082269 E009 10.4705308 0.0016001715 4.559010e-01 5.963888e-01 6 70413544 70413549 6 + 0.884 0.852 -0.123
ENSG00000082269 E010 12.6546257 0.0016228645 8.659207e-01 9.176633e-01 6 70413550 70413565 16 + 0.944 1.007 0.233
ENSG00000082269 E011 13.2480020 0.0013540188 7.495399e-01 8.364636e-01 6 70413566 70413577 12 + 0.964 1.007 0.158
ENSG00000082269 E012 17.2222285 0.0011609896 7.950541e-01 8.690840e-01 6 70413578 70413645 68 + 1.065 1.121 0.205
ENSG00000082269 E013 22.3423197 0.0008943189 7.402464e-01 8.297092e-01 6 70413646 70413702 57 + 1.162 1.286 0.439
ENSG00000082269 E014 25.5382779 0.0007911483 3.763844e-02 8.665285e-02 6 70415291 70415376 86 + 1.192 1.470 0.970
ENSG00000082269 E015 12.1674306 0.0039577752 5.403573e-29 8.322689e-27 6 70417280 70417482 203 + 0.517 1.707 4.449
ENSG00000082269 E016 0.3030308 0.3303644756 1.000000e+00   6 70418375 70418492 118 + 0.082 0.000 -9.583
ENSG00000082269 E017 17.7466354 0.0070383071 2.099527e-03 7.532557e-03 6 70426439 70426527 89 + 1.116 0.705 -1.567
ENSG00000082269 E018 10.5217511 0.0021667507 8.337885e-03 2.463678e-02 6 70426528 70426532 5 + 0.912 0.482 -1.817
ENSG00000082269 E019 5.3558508 0.0136244878 6.386536e-02 1.331312e-01 6 70426570 70426709 140 + 0.667 0.305 -1.840
ENSG00000082269 E020 1.9431837 0.1227745422 7.490472e-01 8.361060e-01 6 70426710 70426764 55 + 0.353 0.303 -0.308
ENSG00000082269 E021 0.0000000       6 70426765 70426771 7 +      
ENSG00000082269 E022 15.7292070 0.0011634638 5.241603e-03 1.658936e-02 6 70428304 70428342 39 + 1.065 0.705 -1.381
ENSG00000082269 E023 18.8665300 0.0121520744 1.508167e-02 4.067098e-02 6 70428343 70428385 43 + 1.133 0.851 -1.048
ENSG00000082269 E024 13.0719389 0.0085691053 1.724749e-01 2.914145e-01 6 70428386 70428419 34 + 0.979 0.851 -0.484
ENSG00000082269 E025 21.4272352 0.0008634608 6.620287e-03 2.024068e-02 6 70452492 70452571 80 + 1.183 0.910 -0.999
ENSG00000082269 E026 18.4703856 0.0062036329 4.030595e-02 9.159834e-02 6 70475410 70475458 49 + 1.116 0.910 -0.758
ENSG00000082269 E027 17.6312360 0.1363425301 2.669047e-01 4.051658e-01 6 70475459 70475549 91 + 1.091 0.967 -0.455
ENSG00000082269 E028 23.9491467 0.0011699367 1.927871e-03 7.001810e-03 6 70475663 70475733 71 + 1.228 0.910 -1.158
ENSG00000082269 E029 37.6816550 0.0421162636 1.268544e-01 2.297663e-01 6 70477159 70477292 134 + 1.401 1.289 -0.389
ENSG00000082269 E030 25.5723958 0.0062005239 4.230679e-02 9.528497e-02 6 70477293 70477332 40 + 1.244 1.087 -0.562
ENSG00000082269 E031 40.2244838 0.0018704992 9.321190e-05 4.901386e-04 6 70480901 70481027 127 + 1.441 1.121 -1.123
ENSG00000082269 E032 35.1122093 0.0007080307 6.941211e-02 1.423891e-01 6 70482001 70482132 132 + 1.368 1.286 -0.286
ENSG00000082269 E033 15.8525025 0.0013407487 5.852624e-01 7.089815e-01 6 70482133 70482154 22 + 1.032 1.049 0.060
ENSG00000082269 E034 20.9588401 0.0009971135 1.978273e-01 3.235055e-01 6 70486167 70486244 78 + 1.152 1.087 -0.237
ENSG00000082269 E035 23.0709315 0.0009266021 4.582724e-02 1.017156e-01 6 70491034 70491083 50 + 1.201 1.049 -0.546
ENSG00000082269 E036 39.2747536 0.0047099976 1.572347e-03 5.868794e-03 6 70502636 70502791 156 + 1.428 1.184 -0.852
ENSG00000082269 E037 12.9909834 0.0014420442 1.396785e-04 7.021803e-04 6 70502792 70504960 2169 + 0.866 1.369 1.821
ENSG00000082269 E038 28.4636296 0.0209366062 1.400516e-03 5.308596e-03 6 70522513 70522586 74 + 1.303 0.911 -1.418
ENSG00000082269 E039 40.4839843 0.0115049775 2.375026e-03 8.383641e-03 6 70523967 70524121 155 + 1.443 1.183 -0.906
ENSG00000082269 E040 0.1472490 0.0455260538 7.076384e-01   6 70524122 70524342 221 + 0.043 0.000 -9.315
ENSG00000082269 E041 16.8390030 0.0074891361 8.930282e-02 1.740765e-01 6 70524343 70524930 588 + 1.073 0.911 -0.599
ENSG00000082269 E042 58.6613573 0.0011443721 3.355215e-02 7.887747e-02 6 70524931 70525191 261 + 1.581 1.513 -0.233
ENSG00000082269 E043 53.2731834 0.0252628732 9.103803e-02 1.767714e-01 6 70525192 70525863 672 + 1.545 1.455 -0.305
ENSG00000082269 E044 133.5663717 0.0002520560 8.747995e-03 2.565499e-02 6 70525864 70526698 835 + 1.930 1.900 -0.099
ENSG00000082269 E045 43.5637582 0.0006383867 8.145521e-01 8.825230e-01 6 70528292 70528452 161 + 1.440 1.513 0.252
ENSG00000082269 E046 34.2685515 0.0006310799 7.695100e-01 8.511389e-01 6 70533160 70533251 92 + 1.340 1.406 0.226
ENSG00000082269 E047 30.5875911 0.0006273744 6.852886e-01 7.880659e-01 6 70533757 70533854 98 + 1.297 1.350 0.185
ENSG00000082269 E048 2.2185227 0.2868684343 8.042304e-02 1.602244e-01 6 70535907 70536259 353 + 0.263 0.802 2.681
ENSG00000082269 E049 46.7542514 0.0055957396 5.844495e-01 7.083792e-01 6 70536260 70536411 152 + 1.462 1.599 0.470
ENSG00000082269 E050 36.7598686 0.0029882672 9.001325e-01 9.402650e-01 6 70538291 70538401 111 + 1.372 1.454 0.284
ENSG00000082269 E051 0.2998086 0.0288116266 1.372298e-01   6 70556698 70556749 52 + 0.043 0.305 3.289
ENSG00000082269 E052 33.6457409 0.0005903500 6.819458e-01 7.855372e-01 6 70556750 70556863 114 + 1.328 1.455 0.439
ENSG00000082269 E053 2.0167736 0.0577898342 6.585209e-01 7.676274e-01 6 70556864 70557694 831 + 0.331 0.484 0.842
ENSG00000082269 E054 14.8240701 0.0011530328 2.156290e-01 3.451620e-01 6 70559716 70559720 5 + 0.979 1.212 0.841
ENSG00000082269 E055 114.8965445 0.0637925203 1.824892e-06 1.432206e-05 6 70559721 70561174 1454 + 1.716 2.388 2.253