ENSG00000082258

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000264157 ENSG00000082258 HEK293_OSMI2_2hA HEK293_TMG_2hB CCNT2 protein_coding protein_coding 10.71594 1.42974 18.55852 0.178363 1.149858 3.688979 2.81609580 0.53093911 4.6220550 0.080600708 0.2260560 3.098114 0.36986250 0.39506667 0.2496667 -0.14540000 4.519209e-01 2.941446e-14 FALSE TRUE
ENST00000417175 ENSG00000082258 HEK293_OSMI2_2hA HEK293_TMG_2hB CCNT2 protein_coding nonsense_mediated_decay 10.71594 1.42974 18.55852 0.178363 1.149858 3.688979 1.09486387 0.05004936 1.9429360 0.050049355 0.1064695 5.023352 0.07397500 0.03650000 0.1061333 0.06963333 1.673319e-01 2.941446e-14 TRUE TRUE
ENST00000446247 ENSG00000082258 HEK293_OSMI2_2hA HEK293_TMG_2hB CCNT2 protein_coding protein_coding 10.71594 1.42974 18.55852 0.178363 1.149858 3.688979 3.05978451 0.00000000 5.5857151 0.000000000 0.1578434 9.128179 0.17535833 0.00000000 0.3023333 0.30233333 2.941446e-14 2.941446e-14 FALSE TRUE
ENST00000452521 ENSG00000082258 HEK293_OSMI2_2hA HEK293_TMG_2hB CCNT2 protein_coding protein_coding 10.71594 1.42974 18.55852 0.178363 1.149858 3.688979 0.04723620 0.16207429 0.0000000 0.162074287 0.0000000 -4.104960 0.02680000 0.09190000 0.0000000 -0.09190000 2.735295e-01 2.941446e-14 FALSE FALSE
ENST00000475094 ENSG00000082258 HEK293_OSMI2_2hA HEK293_TMG_2hB CCNT2 protein_coding retained_intron 10.71594 1.42974 18.55852 0.178363 1.149858 3.688979 1.30972554 0.00000000 2.4476603 0.000000000 0.9272125 7.941142 0.08011250 0.00000000 0.1269667 0.12696667 1.465506e-03 2.941446e-14 FALSE TRUE
MSTRG.19268.4 ENSG00000082258 HEK293_OSMI2_2hA HEK293_TMG_2hB CCNT2 protein_coding   10.71594 1.42974 18.55852 0.178363 1.149858 3.688979 0.04435474 0.22586419 0.0000000 0.225864188 0.0000000 -4.559884 0.01670000 0.12806667 0.0000000 -0.12806667 3.109940e-01 2.941446e-14 FALSE TRUE
MSTRG.19268.8 ENSG00000082258 HEK293_OSMI2_2hA HEK293_TMG_2hB CCNT2 protein_coding   10.71594 1.42974 18.55852 0.178363 1.149858 3.688979 1.59742538 0.25976828 2.6390466 0.138052322 0.2102263 3.295681 0.14507917 0.21096667 0.1445667 -0.06640000 1.000000e+00 2.941446e-14 FALSE TRUE
MSTRG.19268.9 ENSG00000082258 HEK293_OSMI2_2hA HEK293_TMG_2hB CCNT2 protein_coding   10.71594 1.42974 18.55852 0.178363 1.149858 3.688979 0.40380304 0.08881107 0.9418774 0.009367223 0.2572245 3.268031 0.05901667 0.06336667 0.0497000 -0.01366667 8.601263e-01 2.941446e-14 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000082258 E001 0.000000       2 134918235 134918240 6 +      
ENSG00000082258 E002 0.000000       2 134918241 134918297 57 +      
ENSG00000082258 E003 0.000000       2 134918298 134918336 39 +      
ENSG00000082258 E004 1.556377 0.0087815571 4.268576e-01 5.696827e-01 2 134918337 134918821 485 + 0.240 0.519 1.642
ENSG00000082258 E005 0.000000       2 134918822 134918823 2 +      
ENSG00000082258 E006 0.294498 0.3303621993 1.000000e+00   2 134918824 134918824 1 + 0.073 0.001 -6.781
ENSG00000082258 E007 1.703639 0.0431668181 5.065924e-01 6.422480e-01 2 134918825 134918836 12 + 0.262 0.519 1.473
ENSG00000082258 E008 3.332025 0.0647911391 7.879013e-01 8.641403e-01 2 134918837 134918842 6 + 0.454 0.519 0.321
ENSG00000082258 E009 6.147163 0.0046641603 1.545091e-01 2.677676e-01 2 134918843 134918846 4 + 0.662 0.519 -0.643
ENSG00000082258 E010 8.891325 0.0022322053 9.842391e-02 1.881147e-01 2 134918847 134918855 9 + 0.789 0.648 -0.580
ENSG00000082258 E011 14.914256 0.0011992842 3.073912e-02 7.340123e-02 2 134918856 134918905 50 + 0.985 0.829 -0.590
ENSG00000082258 E012 25.145806 0.0017946663 2.490606e-01 3.848062e-01 2 134918906 134919012 107 + 1.173 1.233 0.212
ENSG00000082258 E013 31.192919 0.0006487640 4.884615e-01 6.260152e-01 2 134919810 134919891 82 + 1.256 1.380 0.433
ENSG00000082258 E014 0.000000       2 134919892 134920362 471 +      
ENSG00000082258 E015 48.975382 0.0218110286 2.896978e-02 6.994772e-02 2 134936841 134936969 129 + 1.461 1.406 -0.189
ENSG00000082258 E016 44.920404 0.0005365937 3.501929e-04 1.580891e-03 2 134939002 134939062 61 + 1.432 1.288 -0.502
ENSG00000082258 E017 43.282784 0.0005230428 1.129493e-04 5.814489e-04 2 134942612 134942674 63 + 1.420 1.233 -0.653
ENSG00000082258 E018 6.558511 0.0632246226 6.159514e-03 1.903156e-02 2 134942675 134942769 95 + 0.705 0.001 -11.331
ENSG00000082258 E019 12.605610 0.0013822236 3.990744e-01 5.428202e-01 2 134945787 134945888 102 + 0.903 0.957 0.202
ENSG00000082258 E020 13.592548 0.0012681080 6.127790e-02 1.287890e-01 2 134945889 134945975 87 + 0.946 0.829 -0.445
ENSG00000082258 E021 3.619351 0.0044141965 2.168811e-01 3.466399e-01 2 134945976 134945982 7 + 0.494 0.332 -0.881
ENSG00000082258 E022 44.134175 0.0005233029 4.915456e-04 2.128110e-03 2 134946101 134946146 46 + 1.425 1.288 -0.477
ENSG00000082258 E023 42.779495 0.0050196391 9.236794e-04 3.697978e-03 2 134946147 134946465 319 + 1.413 1.262 -0.525
ENSG00000082258 E024 7.002983 0.0180005868 9.935008e-01 1.000000e+00 2 134947730 134947735 6 + 0.671 0.831 0.649
ENSG00000082258 E025 57.890104 0.0023645539 5.219181e-05 2.927226e-04 2 134947736 134947825 90 + 1.540 1.380 -0.550
ENSG00000082258 E026 47.362017 0.0005093729 5.807123e-04 2.462600e-03 2 134947826 134947899 74 + 1.454 1.336 -0.407
ENSG00000082258 E027 53.341436 0.0025188833 3.205609e-03 1.087248e-02 2 134952641 134952711 71 + 1.498 1.439 -0.203
ENSG00000082258 E028 1.143673 0.0110220581 3.573643e-03 1.193758e-02 2 134952712 134953198 487 + 0.106 0.748 4.057
ENSG00000082258 E029 38.647738 0.0504692363 4.389367e-02 9.818778e-02 2 134953230 134953268 39 + 1.365 1.270 -0.334
ENSG00000082258 E030 261.036048 0.0001769182 1.512492e-05 9.645763e-05 2 134953269 134954379 1111 + 2.169 2.225 0.186
ENSG00000082258 E031 29.573346 0.0006820173 4.950309e-01 6.319081e-01 2 134954380 134954480 101 + 1.236 1.359 0.431
ENSG00000082258 E032 542.909774 0.0419639686 4.377248e-05 2.500773e-04 2 134954481 134959342 4862 + 2.430 2.858 1.425