ENSG00000081721

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000367943 ENSG00000081721 HEK293_OSMI2_2hA HEK293_TMG_2hB DUSP12 protein_coding protein_coding 18.16061 14.00933 25.56061 1.732894 0.610834 0.8670687 11.527006 10.7886604 14.593479 1.2979699 0.2502078 0.4354598 0.6570667 0.77120000 0.5711333 -0.20006667 9.636965e-08 9.636965e-08 FALSE TRUE
ENST00000463365 ENSG00000081721 HEK293_OSMI2_2hA HEK293_TMG_2hB DUSP12 protein_coding retained_intron 18.16061 14.00933 25.56061 1.732894 0.610834 0.8670687 2.158879 0.4491639 3.385689 0.1492882 0.1229868 2.8866231 0.1081250 0.03386667 0.1324000 0.09853333 9.100198e-04 9.636965e-08   FALSE
ENST00000464004 ENSG00000081721 HEK293_OSMI2_2hA HEK293_TMG_2hB DUSP12 protein_coding nonsense_mediated_decay 18.16061 14.00933 25.56061 1.732894 0.610834 0.8670687 3.923390 2.2645436 6.742017 0.5996047 0.3765179 1.5697415 0.2055667 0.15776667 0.2635000 0.10573333 1.144505e-01 9.636965e-08 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000081721 E001 0.9492964 0.0357615471 0.7276374156 0.8202724593 1 161748502 161749757 1256 + 0.266 0.328 0.421
ENSG00000081721 E002 2.7699931 0.0058927641 0.8946311181 0.9366806206 1 161749758 161749779 22 + 0.581 0.561 -0.092
ENSG00000081721 E003 4.9938409 0.0032260014 0.6437926628 0.7562476041 1 161749780 161749785 6 + 0.802 0.742 -0.241
ENSG00000081721 E004 16.6159604 0.0022778682 0.4338212815 0.5762272508 1 161749786 161749804 19 + 1.279 1.216 -0.222
ENSG00000081721 E005 129.7038054 0.0042994433 0.2266081880 0.3583522395 1 161749805 161750088 284 + 2.143 2.088 -0.186
ENSG00000081721 E006 75.2090927 0.0034821313 0.8866346359 0.9314966581 1 161750089 161750145 57 + 1.888 1.879 -0.028
ENSG00000081721 E007 2.1370639 0.1372982601 0.6389249132 0.7524522148 1 161751132 161751377 246 + 0.429 0.524 0.473
ENSG00000081721 E008 23.1168265 0.0017707873 0.0108688980 0.0308527265 1 161751655 161751667 13 + 1.464 1.280 -0.638
ENSG00000081721 E009 122.0027580 0.0015956068 0.2697790743 0.4083343917 1 161751668 161751781 114 + 2.110 2.071 -0.130
ENSG00000081721 E010 133.4540125 0.0004255776 0.8568293071 0.9115255311 1 161751866 161751984 119 + 2.133 2.130 -0.010
ENSG00000081721 E011 86.3147833 0.0003634496 0.4670175088 0.6065336668 1 161752368 161752398 31 + 1.931 1.961 0.099
ENSG00000081721 E012 118.6919928 0.0028273136 0.7522185262 0.8384849204 1 161752399 161752464 66 + 2.077 2.091 0.049
ENSG00000081721 E013 184.7332228 0.0010545822 0.5620177228 0.6897207253 1 161753075 161753261 187 + 2.265 2.283 0.058
ENSG00000081721 E014 8.4572985 0.0020175081 0.0001216716 0.0006214533 1 161753262 161753406 145 + 1.135 0.678 -1.748
ENSG00000081721 E015 188.0921152 0.0017071273 0.0053625344 0.0169163131 1 161756786 161757238 453 + 2.241 2.323 0.274