ENSG00000081087

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000193322 ENSG00000081087 HEK293_OSMI2_2hA HEK293_TMG_2hB OSTM1 protein_coding protein_coding 11.21284 1.710545 17.13073 0.309648 1.35272 3.316491 1.06411421 0.30334587 1.84262486 0.13365982 0.19777365 2.5637429 0.14675417 0.16243333 0.107733333 -0.05470000 3.330216e-01 3.054304e-05 FALSE TRUE
ENST00000440575 ENSG00000081087 HEK293_OSMI2_2hA HEK293_TMG_2hB OSTM1 protein_coding protein_coding 11.21284 1.710545 17.13073 0.309648 1.35272 3.316491 1.54575663 0.00000000 1.56534923 0.00000000 0.19595302 7.2995279 0.07260833 0.00000000 0.091300000 0.09130000 3.850668e-05 3.054304e-05 FALSE FALSE
ENST00000467960 ENSG00000081087 HEK293_OSMI2_2hA HEK293_TMG_2hB OSTM1 protein_coding processed_transcript 11.21284 1.710545 17.13073 0.309648 1.35272 3.316491 0.09333673 0.09361504 0.09089767 0.01092511 0.04547677 -0.0383407 0.02841667 0.05610000 0.005000000 -0.05110000 1.166106e-03 3.054304e-05 TRUE TRUE
ENST00000472669 ENSG00000081087 HEK293_OSMI2_2hA HEK293_TMG_2hB OSTM1 protein_coding processed_transcript 11.21284 1.710545 17.13073 0.309648 1.35272 3.316491 1.17849407 0.04465091 2.16208004 0.04465091 0.01652937 5.3126880 0.07848750 0.01916667 0.127933333 0.10876667 8.904054e-03 3.054304e-05 FALSE FALSE
ENST00000477774 ENSG00000081087 HEK293_OSMI2_2hA HEK293_TMG_2hB OSTM1 protein_coding processed_transcript 11.21284 1.710545 17.13073 0.309648 1.35272 3.316491 2.80964320 0.00000000 3.93787166 0.00000000 1.25260392 8.6249313 0.13181250 0.00000000 0.220933333 0.22093333 3.054304e-05 3.054304e-05   FALSE
ENST00000492070 ENSG00000081087 HEK293_OSMI2_2hA HEK293_TMG_2hB OSTM1 protein_coding processed_transcript 11.21284 1.710545 17.13073 0.309648 1.35272 3.316491 0.04460088 0.11109950 0.03468736 0.05760272 0.03468736 -1.4382540 0.02549167 0.06613333 0.002366667 -0.06376667 7.625237e-02 3.054304e-05   FALSE
ENST00000492130 ENSG00000081087 HEK293_OSMI2_2hA HEK293_TMG_2hB OSTM1 protein_coding processed_transcript 11.21284 1.710545 17.13073 0.309648 1.35272 3.316491 0.07234316 0.11757173 0.00000000 0.11757173 0.00000000 -3.6732368 0.03388750 0.05046667 0.000000000 -0.05046667 2.425806e-01 3.054304e-05   FALSE
MSTRG.28745.2 ENSG00000081087 HEK293_OSMI2_2hA HEK293_TMG_2hB OSTM1 protein_coding   11.21284 1.710545 17.13073 0.309648 1.35272 3.316491 4.40454955 1.04026167 7.49721891 0.04881017 0.30421694 2.8375297 0.48255417 0.64573333 0.444700000 -0.20103333 3.632114e-01 3.054304e-05 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000081087 E001 0.2987644 0.0293846619 1.000000e+00   6 108041409 108041471 63 - 0.093 0.000 -9.214
ENSG00000081087 E002 32.5574802 0.0006303915 8.780654e-03 2.573435e-02 6 108041472 108042264 793 - 1.344 1.550 0.712
ENSG00000081087 E003 10.1477260 0.0020637921 4.174362e-02 9.426387e-02 6 108042265 108042566 302 - 0.866 1.127 0.965
ENSG00000081087 E004 246.0071908 0.0002797456 8.738429e-29 1.321204e-26 6 108042567 108044378 1812 - 2.188 2.461 0.913
ENSG00000081087 E005 72.4682741 0.0003541775 2.144979e-04 1.026265e-03 6 108044379 108044732 354 - 1.680 1.877 0.664
ENSG00000081087 E006 91.4785036 0.0003723226 1.559810e-05 9.914822e-05 6 108044733 108044830 98 - 1.839 1.582 -0.870
ENSG00000081087 E007 62.7286618 0.0004400920 2.225324e-05 1.364870e-04 6 108044831 108044840 10 - 1.682 1.364 -1.091
ENSG00000081087 E008 107.9616616 0.0008443009 1.312548e-07 1.303672e-06 6 108049253 108049418 166 - 1.913 1.611 -1.022
ENSG00000081087 E009 2.2312252 0.0205152137 5.066820e-01 6.423359e-01 6 108049532 108049583 52 - 0.427 0.275 -0.923
ENSG00000081087 E010 0.2987644 0.0293846619 1.000000e+00   6 108049584 108049668 85 - 0.093 0.000 -10.913
ENSG00000081087 E011 87.5270755 0.0003929841 3.857026e-05 2.231696e-04 6 108051031 108051198 168 - 1.820 1.572 -0.843
ENSG00000081087 E012 54.5657791 0.0007250968 3.653536e-03 1.216542e-02 6 108054490 108054587 98 - 1.616 1.396 -0.755
ENSG00000081087 E013 52.5784865 0.0015197887 1.451040e-02 3.938666e-02 6 108064185 108064299 115 - 1.598 1.411 -0.642
ENSG00000081087 E014 1.4392153 0.0094746183 1.880707e-03 6.854979e-03 6 108073642 108073798 157 - 0.170 0.734 3.208
ENSG00000081087 E015 79.4553680 0.0003780146 1.629318e-04 8.044762e-04 6 108074250 108074741 492 - 1.777 1.540 -0.807
ENSG00000081087 E016 0.3299976 0.0274424043 5.589598e-03   6 108122336 108122714 379 - 0.000 0.442 13.913
ENSG00000081087 E017 0.0000000       6 108165567 108165704 138 -      
ENSG00000081087 E018 0.0000000       6 108165812 108165854 43 -