ENSG00000081019

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000261441 ENSG00000081019 HEK293_OSMI2_2hA HEK293_TMG_2hB RSBN1 protein_coding protein_coding 4.727799 1.617125 7.751441 0.3061363 0.4189266 2.253999 0.3721896 1.1911316 0.00000000 0.6478142 0.00000000 -6.9082504 0.1491250 0.66666667 0.0000000 -0.66666667 2.958725e-01 1.75662e-14 FALSE TRUE
ENST00000369581 ENSG00000081019 HEK293_OSMI2_2hA HEK293_TMG_2hB RSBN1 protein_coding processed_transcript 4.727799 1.617125 7.751441 0.3061363 0.4189266 2.253999 0.1541677 0.1145717 0.07390213 0.1145717 0.07390213 -0.5701971 0.0385875 0.08966667 0.0087000 -0.08096667 8.205941e-01 1.75662e-14 FALSE FALSE
ENST00000612242 ENSG00000081019 HEK293_OSMI2_2hA HEK293_TMG_2hB RSBN1 protein_coding protein_coding 4.727799 1.617125 7.751441 0.3061363 0.4189266 2.253999 3.0162905 0.3114218 5.31258495 0.3114218 0.19313905 4.0495876 0.6363083 0.24366667 0.6867667 0.44310000 1.503506e-01 1.75662e-14 FALSE TRUE
ENST00000615321 ENSG00000081019 HEK293_OSMI2_2hA HEK293_TMG_2hB RSBN1 protein_coding protein_coding 4.727799 1.617125 7.751441 0.3061363 0.4189266 2.253999 1.1452492 0.0000000 2.33286850 0.0000000 0.19838863 7.8721322 0.1678292 0.00000000 0.2999667 0.29996667 1.756620e-14 1.75662e-14 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000081019 E001 0.5514428 0.0217681645 5.109922e-03 0.0162295366 1 113761832 113761832 1 - 0.000 0.465 13.054
ENSG00000081019 E002 235.6001258 0.0088325319 1.467991e-04 0.0007332869 1 113761833 113764615 2783 - 2.256 2.448 0.641
ENSG00000081019 E003 117.3303654 0.0003566816 8.071329e-01 0.8774917539 1 113764616 113765847 1232 - 2.000 2.051 0.172
ENSG00000081019 E004 61.0436598 0.0004737935 4.573042e-01 0.5976496230 1 113765848 113766144 297 - 1.711 1.790 0.266
ENSG00000081019 E005 84.5441865 0.0215081655 6.393519e-01 0.7527515401 1 113766145 113766453 309 - 1.871 1.841 -0.101
ENSG00000081019 E006 41.5604157 0.0005703821 6.509288e-01 0.7619043244 1 113767099 113767207 109 - 1.560 1.573 0.043
ENSG00000081019 E007 1.1040209 0.0116248996 6.970376e-01 0.7972739414 1 113767955 113768221 267 - 0.262 0.357 0.623
ENSG00000081019 E008 39.4094480 0.0006636246 3.856064e-02 0.0883860752 1 113768222 113768389 168 - 1.562 1.464 -0.337
ENSG00000081019 E009 39.4209229 0.0006150086 3.294519e-01 0.4729597838 1 113777210 113777352 143 - 1.548 1.526 -0.077
ENSG00000081019 E010 37.2739132 0.0007674966 1.332380e-01 0.2386302504 1 113777671 113777808 138 - 1.534 1.473 -0.208
ENSG00000081019 E011 0.2987644 0.0280098570 1.000000e+00   1 113796186 113796303 118 - 0.124 0.000 -8.855
ENSG00000081019 E012 91.5595314 0.0042226623 7.818062e-05 0.0004193518 1 113797363 113798036 674 - 1.937 1.756 -0.607
ENSG00000081019 E013 50.3080128 0.0016875363 2.231130e-05 0.0001368076 1 113811710 113812476 767 - 1.691 1.454 -0.810