ENSG00000080986

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000261597 ENSG00000080986 HEK293_OSMI2_2hA HEK293_TMG_2hB NDC80 protein_coding protein_coding 16.4346 6.239948 25.43675 0.333569 0.6523529 2.025565 14.8203280 4.9872852 23.14554 0.2603732 0.21917024 2.2121410 0.87754167 0.7996667 0.9109667 0.11130000 0.03152552 0.0250745 FALSE TRUE
ENST00000576274 ENSG00000080986 HEK293_OSMI2_2hA HEK293_TMG_2hB NDC80 protein_coding protein_coding 16.4346 6.239948 25.43675 0.333569 0.6523529 2.025565 0.6732656 0.8086956 1.03133 0.2483762 0.07640132 0.3470284 0.05466667 0.1268333 0.0405000 -0.08633333 0.02507450 0.0250745 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000080986 E001 6.3119267 0.0025885372 5.240955e-01 0.6577215429 18 2571557 2571564 8 + 0.829 0.741 -0.349
ENSG00000080986 E002 67.7584559 0.0004052876 1.215313e-01 0.2221125999 18 2571565 2571683 119 + 1.784 1.710 -0.249
ENSG00000080986 E003 91.8008958 0.0002892695 4.842454e-02 0.1063724946 18 2572977 2573086 110 + 1.914 1.833 -0.274
ENSG00000080986 E004 75.8350540 0.0003462140 2.348309e-02 0.0587447870 18 2574989 2575066 78 + 1.838 1.732 -0.358
ENSG00000080986 E005 0.2966881 0.0270142668 1.000000e+00   18 2577236 2577357 122 + 0.123 0.001 -7.189
ENSG00000080986 E006 87.7557896 0.0004349014 7.043222e-02 0.1440651762 18 2577746 2577848 103 + 1.893 1.816 -0.258
ENSG00000080986 E007 50.6223217 0.0006500381 3.893606e-02 0.0890698551 18 2577849 2577869 21 + 1.667 1.546 -0.412
ENSG00000080986 E008 103.4967778 0.0003113079 1.562358e-02 0.0418956033 18 2577969 2578122 154 + 1.968 1.872 -0.322
ENSG00000080986 E009 42.3776590 0.0010432983 3.542415e-02 0.0824710582 18 2578123 2578141 19 + 1.596 1.459 -0.470
ENSG00000080986 E010 88.8499477 0.0003358491 3.614116e-04 0.0016244357 18 2578927 2579029 103 + 1.914 1.752 -0.548
ENSG00000080986 E011 2.2981483 0.0064400725 1.365613e-01 0.2432731681 18 2579030 2579196 167 + 0.518 0.216 -1.833
ENSG00000080986 E012 20.0856570 0.0382275469 2.374376e-05 0.0001447618 18 2582102 2582879 778 + 1.061 1.595 1.868
ENSG00000080986 E013 81.7332568 0.0059541627 3.101057e-02 0.0739366570 18 2585113 2585202 90 + 1.870 1.745 -0.419
ENSG00000080986 E014 77.4560038 0.0013039659 1.404703e-01 0.2486770534 18 2587830 2587923 94 + 1.836 1.767 -0.231
ENSG00000080986 E015 90.1594904 0.0004403586 9.987557e-02 0.1902902137 18 2589204 2589310 107 + 1.901 1.833 -0.229
ENSG00000080986 E016 113.0113077 0.0003073676 9.856882e-01 0.9950797699 18 2590018 2590162 145 + 1.983 1.990 0.026
ENSG00000080986 E017 134.5405856 0.0002421740 1.502126e-01 0.2619632192 18 2595416 2595621 206 + 2.046 2.103 0.191
ENSG00000080986 E018 87.0844097 0.0003638549 1.571568e-01 0.2713161927 18 2599019 2599171 153 + 1.857 1.925 0.230
ENSG00000080986 E019 90.5915780 0.0003349107 5.054928e-02 0.1101331736 18 2601396 2601485 90 + 1.868 1.957 0.302
ENSG00000080986 E020 108.4294954 0.0006494398 1.558597e-02 0.0418142774 18 2606415 2606507 93 + 1.941 2.044 0.344
ENSG00000080986 E021 131.0464968 0.0016104349 5.879239e-03 0.0182947750 18 2608700 2608830 131 + 2.021 2.135 0.380
ENSG00000080986 E022 64.0923992 0.0016568546 6.231014e-01 0.7397766817 18 2610759 2610780 22 + 1.737 1.771 0.115
ENSG00000080986 E023 89.1939124 0.0109347293 1.716665e-01 0.2903879106 18 2610781 2610861 81 + 1.861 1.962 0.338
ENSG00000080986 E024 4.4149614 0.0041294938 5.880276e-03 0.0182970995 18 2614954 2615314 361 + 0.539 0.942 1.653
ENSG00000080986 E025 71.0640130 0.0019623497 1.101084e-03 0.0043067430 18 2616437 2616635 199 + 1.744 1.914 0.573