ENSG00000077721

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000371558 ENSG00000077721 HEK293_OSMI2_2hA HEK293_TMG_2hB UBE2A protein_coding protein_coding 72.63241 95.11083 71.69617 4.855931 1.272031 -0.4076639 57.419961 82.4204703 54.769488 5.33426491 1.4397174 -0.589542 0.78134167 0.865400000 0.76376667 -0.10163333 2.254297e-04 3.159776e-25 FALSE TRUE
ENST00000469205 ENSG00000077721 HEK293_OSMI2_2hA HEK293_TMG_2hB UBE2A protein_coding retained_intron 72.63241 95.11083 71.69617 4.855931 1.272031 -0.4076639 3.069934 0.7178201 4.725545 0.09234687 0.1927646 2.701877 0.04657083 0.007633333 0.06596667 0.05833333 3.159776e-25 3.159776e-25 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000077721 E001 0.5911836 3.967766e-02 2.315031e-01 3.642603e-01 X 119474802 119474894 93 + 0.001 0.229 9.043
ENSG00000077721 E002 0.0000000       X 119542540 119542651 112 +      
ENSG00000077721 E003 0.0000000       X 119572949 119573166 218 +      
ENSG00000077721 E004 0.0000000       X 119573643 119573820 178 +      
ENSG00000077721 E005 0.7717566 2.621338e-02 4.800105e-01 6.183342e-01 X 119574146 119574160 15 + 0.164 0.283 1.004
ENSG00000077721 E006 1.1017542 3.317858e-02 2.392495e-01 3.732365e-01 X 119574161 119574316 156 + 0.164 0.376 1.586
ENSG00000077721 E007 4.3029949 4.400236e-03 7.554713e-01 8.407899e-01 X 119574517 119574525 9 + 0.669 0.693 0.098
ENSG00000077721 E008 9.2797901 1.871445e-03 5.925398e-01 7.150730e-01 X 119574526 119574535 10 + 0.944 0.980 0.133
ENSG00000077721 E009 12.2833552 4.359522e-03 1.514444e-01 2.636357e-01 X 119574536 119574545 10 + 0.987 1.115 0.465
ENSG00000077721 E010 16.1708349 2.363760e-02 8.140156e-01 8.821386e-01 X 119574546 119574548 3 + 1.232 1.175 -0.199
ENSG00000077721 E011 39.6307518 4.807647e-03 1.560029e-02 4.184796e-02 X 119574549 119574562 14 + 1.462 1.601 0.477
ENSG00000077721 E012 46.8644252 1.155917e-03 2.463026e-03 8.652836e-03 X 119574563 119574570 8 + 1.526 1.676 0.512
ENSG00000077721 E013 206.4676691 7.058430e-03 4.015044e-02 9.131490e-02 X 119574571 119574577 7 + 2.361 2.233 -0.427
ENSG00000077721 E014 763.9171392 2.128242e-03 2.471510e-01 3.825734e-01 X 119574578 119574747 170 + 2.844 2.842 -0.008
ENSG00000077721 E015 407.7494241 1.648029e-03 4.888202e-02 1.071662e-01 X 119574748 119574755 8 + 2.555 2.577 0.072
ENSG00000077721 E016 6.3223283 2.668277e-03 4.897034e-04 2.120958e-03 X 119574756 119574900 145 + 1.096 0.650 -1.728
ENSG00000077721 E017 358.1710393 2.523471e-03 2.042307e-01 3.314591e-01 X 119574901 119574907 7 + 2.508 2.517 0.027
ENSG00000077721 E018 697.9736503 1.137263e-03 1.081028e-03 4.239061e-03 X 119574908 119574981 74 + 2.776 2.814 0.125
ENSG00000077721 E019 54.5581929 3.283115e-02 1.730150e-01 2.921109e-01 X 119574982 119575374 393 + 1.821 1.648 -0.586
ENSG00000077721 E020 551.6805549 6.619854e-04 1.259148e-03 4.840343e-03 X 119575375 119575400 26 + 2.677 2.711 0.114
ENSG00000077721 E021 34.8958336 5.603510e-04 1.398114e-33 3.076289e-31 X 119575401 119575794 394 + 1.895 1.144 -2.584
ENSG00000077721 E022 9.6426425 1.284462e-02 7.011431e-15 2.364776e-13 X 119576945 119577066 122 + 1.426 0.454 -3.797
ENSG00000077721 E023 80.7908610 3.217039e-04 4.601072e-55 3.856899e-52 X 119580335 119581506 1172 + 2.217 1.568 -2.184
ENSG00000077721 E024 782.0572477 1.399196e-04 3.136311e-07 2.889771e-06 X 119581507 119581596 90 + 2.823 2.866 0.144
ENSG00000077721 E025 12.6737011 4.753710e-03 3.325376e-06 2.465782e-05 X 119582368 119582587 220 + 1.379 0.911 -1.683
ENSG00000077721 E026 471.8506895 1.475487e-04 4.699378e-04 2.045123e-03 X 119582588 119582596 9 + 2.611 2.645 0.114
ENSG00000077721 E027 862.1879434 9.646483e-05 9.717709e-09 1.201299e-07 X 119582597 119582676 80 + 2.864 2.911 0.155
ENSG00000077721 E028 2559.2555299 6.112479e-03 1.463514e-02 3.967351e-02 X 119583127 119584425 1299 + 3.432 3.339 -0.310
ENSG00000077721 E029 0.6621601 1.652230e-02 5.415885e-01 6.725551e-01 X 119590128 119591083 956 + 0.283 0.165 -0.990