ENSG00000077522

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000542672 ENSG00000077522 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTN2 protein_coding protein_coding 1.635412 1.528328 1.712952 0.05376834 0.07361852 0.1635195 0.16846306 0.16931810 0.31122252 0.12011905 0.16436427 0.84105193 0.10723750 0.11316667 0.18086667 0.06770000 0.87776411 0.01966998 FALSE TRUE
ENST00000651786 ENSG00000077522 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTN2 protein_coding nonsense_mediated_decay 1.635412 1.528328 1.712952 0.05376834 0.07361852 0.1635195 0.04362133 0.30606317 0.00000000 0.30606317 0.00000000 -4.98214101 0.02945417 0.21173333 0.00000000 -0.21173333 0.81440224 0.01966998 FALSE TRUE
ENST00000682966 ENSG00000077522 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTN2 protein_coding retained_intron 1.635412 1.528328 1.712952 0.05376834 0.07361852 0.1635195 0.23332152 0.09712550 0.35189003 0.01277426 0.02117069 1.75624936 0.13864167 0.06413333 0.20696667 0.14283333 0.01966998 0.01966998 FALSE TRUE
ENST00000683111 ENSG00000077522 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTN2 protein_coding nonsense_mediated_decay 1.635412 1.528328 1.712952 0.05376834 0.07361852 0.1635195 0.63909099 0.55254380 0.57291869 0.28562482 0.10429975 0.05132923 0.39622083 0.35046667 0.33686667 -0.01360000 0.96235727 0.01966998 FALSE TRUE
ENST00000683322 ENSG00000077522 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTN2 protein_coding retained_intron 1.635412 1.528328 1.712952 0.05376834 0.07361852 0.1635195 0.08937142 0.04234106 0.09427199 0.01576181 0.01946462 0.99433671 0.05517917 0.02756667 0.05513333 0.02756667 0.65559988 0.01966998 FALSE TRUE
ENST00000684050 ENSG00000077522 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTN2 protein_coding retained_intron 1.635412 1.528328 1.712952 0.05376834 0.07361852 0.1635195 0.13610268 0.08474818 0.06613165 0.04268214 0.03410395 -0.31560193 0.07772917 0.05510000 0.03733333 -0.01776667 0.87006519 0.01966998 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000077522 E001 0.0000000       1 236664141 236664205 65 +      
ENSG00000077522 E002 0.0000000       1 236676866 236677110 245 +      
ENSG00000077522 E003 0.6579068 0.4624957124 9.238567e-01 9.560524e-01 1 236686454 236686498 45 + 0.209 0.228 0.154
ENSG00000077522 E004 0.8051558 0.5970509946 9.149585e-01 9.501509e-01 1 236686499 236686508 10 + 0.285 0.228 -0.428
ENSG00000077522 E005 2.3790495 0.4512528775 3.917243e-01 5.356455e-01 1 236686509 236686605 97 + 0.612 0.436 -0.836
ENSG00000077522 E006 2.0866278 0.4458742833 5.210041e-01 6.549023e-01 1 236686606 236686638 33 + 0.541 0.436 -0.515
ENSG00000077522 E007 2.2391623 0.1833127281 3.354965e-01 4.792767e-01 1 236686639 236686658 20 + 0.612 0.378 -1.156
ENSG00000077522 E008 3.8560165 0.1629237470 6.140856e-01 7.326587e-01 1 236686659 236686673 15 + 0.727 0.623 -0.438
ENSG00000077522 E009 3.3410924 0.2009897255 5.210819e-01 6.549808e-01 1 236686674 236686674 1 + 0.700 0.544 -0.682
ENSG00000077522 E010 3.6355904 0.2526968087 4.663163e-01 6.058722e-01 1 236686675 236686681 7 + 0.751 0.547 -0.878
ENSG00000077522 E011 4.1138811 0.1039703667 6.855172e-01 7.882324e-01 1 236686682 236686688 7 + 0.751 0.651 -0.416
ENSG00000077522 E012 4.9233034 0.1149790212 6.848532e-01 7.877180e-01 1 236686689 236686730 42 + 0.818 0.714 -0.414
ENSG00000077522 E013 4.1192048 0.0405703938 7.661411e-01 8.487158e-01 1 236686731 236686734 4 + 0.727 0.680 -0.198
ENSG00000077522 E014 6.7074659 0.0544469142 8.683710e-01 9.193703e-01 1 236686735 236686799 65 + 0.893 0.861 -0.124
ENSG00000077522 E015 0.0000000       1 236706399 236706619 221 +      
ENSG00000077522 E016 7.0577276 0.0032228033 1.597711e-01 2.748120e-01 1 236717858 236717972 115 + 0.972 0.811 -0.613
ENSG00000077522 E017 4.3222045 0.0039616196 1.257602e-01 2.282296e-01 1 236718894 236718903 10 + 0.818 0.610 -0.859
ENSG00000077522 E018 8.0309084 0.0024536866 6.646337e-01 7.724336e-01 1 236718904 236719013 110 + 0.972 0.930 -0.154
ENSG00000077522 E019 6.5100333 0.0419521362 9.352573e-01 9.632347e-01 1 236720105 236720191 87 + 0.876 0.875 -0.001
ENSG00000077522 E020 2.0293678 0.0081698795 4.789822e-01 6.173928e-01 1 236720192 236720974 783 + 0.407 0.531 0.626
ENSG00000077522 E021 7.5955953 0.0063211969 1.150130e-01 2.128588e-01 1 236725933 236726020 88 + 1.013 0.834 -0.677
ENSG00000077522 E022 1.1748837 0.1606955722 8.024895e-02 1.599415e-01 1 236727678 236727756 79 + 0.500 0.127 -2.677
ENSG00000077522 E023 4.5647687 0.0231321263 8.662950e-02 1.700042e-01 1 236729087 236731232 2146 + 0.858 0.610 -1.015
ENSG00000077522 E024 4.6428536 0.0136168475 3.368018e-02 7.911776e-02 1 236731233 236731249 17 + 0.876 0.573 -1.248
ENSG00000077522 E025 6.8009494 0.0198570817 6.543536e-02 1.357681e-01 1 236731250 236731314 65 + 1.000 0.762 -0.911
ENSG00000077522 E026 16.9367055 0.0018214441 2.669573e-08 3.035670e-07 1 236731315 236734432 3118 + 1.437 0.964 -1.684
ENSG00000077522 E027 3.6733051 0.0184143087 8.535593e-01 9.093806e-01 1 236734433 236734518 86 + 0.644 0.678 0.145
ENSG00000077522 E028 10.5894796 0.0042552092 7.084616e-03 2.144778e-02 1 236734519 236735634 1116 + 1.184 0.912 -0.991
ENSG00000077522 E029 1.8487076 0.0081333665 3.049182e-01 4.469552e-01 1 236735635 236735720 86 + 0.350 0.530 0.947
ENSG00000077522 E030 7.6267928 0.0022133684 8.281911e-03 2.449699e-02 1 236735721 236736557 837 + 1.062 0.762 -1.139
ENSG00000077522 E031 3.1875126 0.1110221358 9.441988e-01 9.690220e-01 1 236736558 236736668 111 + 0.612 0.616 0.019
ENSG00000077522 E032 8.1039350 0.0021803583 3.955327e-01 5.393388e-01 1 236736669 236737121 453 + 1.000 0.913 -0.325
ENSG00000077522 E033 10.3090735 0.0128571217 1.636335e-01 2.798656e-01 1 236737122 236737180 59 + 1.127 0.979 -0.541
ENSG00000077522 E034 10.3180562 0.0018926337 4.240169e-01 5.670171e-01 1 236737181 236737214 34 + 1.096 1.024 -0.262
ENSG00000077522 E035 22.8610602 0.0008070200 8.968042e-01 9.380659e-01 1 236739302 236739532 231 + 1.379 1.378 -0.003
ENSG00000077522 E036 9.2656132 0.0047557091 3.444119e-01 4.885061e-01 1 236742896 236742918 23 + 0.942 1.052 0.405
ENSG00000077522 E037 16.5693857 0.0011510806 2.339409e-01 3.670648e-01 1 236742919 236743043 125 + 1.184 1.289 0.372
ENSG00000077522 E038 15.1184576 0.0012023068 4.947233e-01 6.316517e-01 1 236744626 236744776 151 + 1.175 1.240 0.233
ENSG00000077522 E039 9.6224692 0.0018132352 9.685177e-01 9.843127e-01 1 236747667 236747699 33 + 1.026 1.038 0.043
ENSG00000077522 E040 12.6850003 0.0014805968 2.223133e-01 3.531701e-01 1 236747700 236747775 76 + 1.192 1.089 -0.370
ENSG00000077522 E041 0.4815130 0.0205574142 5.240940e-01 6.577215e-01 1 236747776 236747875 100 + 0.117 0.226 1.139
ENSG00000077522 E042 2.3593669 0.0064587023 6.912818e-01 7.927917e-01 1 236747876 236749123 1248 + 0.501 0.572 0.331
ENSG00000077522 E043 16.8802570 0.0011339256 4.875041e-01 6.251857e-01 1 236749124 236749264 141 + 1.217 1.281 0.224
ENSG00000077522 E044 0.1515154 0.0435289183 5.264900e-01   1 236751199 236751469 271 + 0.117 0.000 -10.887
ENSG00000077522 E045 20.6464801 0.0009325582 2.005735e-02 5.160814e-02 1 236751470 236751652 183 + 1.233 1.414 0.629
ENSG00000077522 E046 0.1482932 0.0413455541 4.537488e-01   1 236753812 236753946 135 + 0.000 0.127 10.392
ENSG00000077522 E047 19.9377331 0.0135696290 8.952388e-02 1.744016e-01 1 236753947 236754081 135 + 1.226 1.395 0.592
ENSG00000077522 E048 1.0350216 0.0134176212 2.985154e-01 4.399664e-01 1 236754254 236754897 644 + 0.407 0.226 -1.184
ENSG00000077522 E049 0.5954526 0.0193874923 4.458558e-02 9.943849e-02 1 236754898 236755018 121 + 0.350 0.000 -12.754
ENSG00000077522 E050 29.0992291 0.0290077182 3.868065e-01 5.309752e-01 1 236755019 236755198 180 + 1.423 1.525 0.353
ENSG00000077522 E051 0.0000000       1 236755382 236755453 72 +      
ENSG00000077522 E052 24.8437744 0.0272655387 1.921827e-01 3.164957e-01 1 236757486 236757632 147 + 1.332 1.481 0.514
ENSG00000077522 E053 18.7120950 0.0223756194 1.013681e-02 2.910032e-02 1 236759724 236759789 66 + 1.127 1.414 1.009
ENSG00000077522 E054 26.0168723 0.0037510675 4.141043e-04 1.828435e-03 1 236761015 236761173 159 + 1.278 1.541 0.909
ENSG00000077522 E055 3.8760918 0.0042934412 1.067904e-01 2.008171e-01 1 236761174 236762460 1287 + 0.797 0.572 -0.948
ENSG00000077522 E056 49.3771146 0.0109222941 8.438395e-02 1.664215e-01 1 236762461 236764631 2171 + 1.635 1.756 0.411