ENSG00000077458

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000358780 ENSG00000077458 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM76B protein_coding protein_coding 10.14167 1.789273 17.65107 0.1832999 0.4070213 3.295087 1.9850188 0.92834058 4.188064 0.34258132 0.14505668 2.161540 0.30137500 0.4895667 0.23736667 -0.25220000 0.213204438 0.001489746 FALSE TRUE
ENST00000398187 ENSG00000077458 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM76B protein_coding nonsense_mediated_decay 10.14167 1.789273 17.65107 0.1832999 0.4070213 3.295087 0.8882604 0.25287899 1.536010 0.17145656 0.33566135 2.556079 0.09722500 0.1649667 0.08770000 -0.07726667 1.000000000 0.001489746 TRUE TRUE
ENST00000535919 ENSG00000077458 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM76B protein_coding retained_intron 10.14167 1.789273 17.65107 0.1832999 0.4070213 3.295087 1.1902813 0.24912058 1.746441 0.05674172 0.06750466 2.760960 0.10210000 0.1384333 0.09900000 -0.03943333 0.526617703 0.001489746 FALSE FALSE
ENST00000536839 ENSG00000077458 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM76B protein_coding protein_coding 10.14167 1.789273 17.65107 0.1832999 0.4070213 3.295087 0.8001916 0.03549308 1.387175 0.02730113 0.35930447 4.940722 0.05515417 0.0230000 0.07813333 0.05513333 0.207913897 0.001489746 FALSE TRUE
ENST00000545813 ENSG00000077458 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM76B protein_coding retained_intron 10.14167 1.789273 17.65107 0.1832999 0.4070213 3.295087 1.0237610 0.00000000 1.959225 0.00000000 0.61784567 7.621484 0.10398333 0.0000000 0.11000000 0.11000000 0.001489746 0.001489746 FALSE TRUE
MSTRG.6203.5 ENSG00000077458 HEK293_OSMI2_2hA HEK293_TMG_2hB FAM76B protein_coding   10.14167 1.789273 17.65107 0.1832999 0.4070213 3.295087 3.7377395 0.18274273 6.023664 0.09235300 0.37152712 4.968286 0.28750833 0.1140333 0.34256667 0.22853333 0.205150745 0.001489746   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000077458 E001 1.3986458 0.3395989131 4.748963e-01 0.6136907065 11 95768953 95768954 2 - 0.246 0.470 1.356
ENSG00000077458 E002 6.5476107 0.0720920938 1.600208e-05 0.0001014795 11 95768955 95768987 33 - 0.517 1.306 3.068
ENSG00000077458 E003 117.4386519 0.0522006950 2.162450e-05 0.0001329924 11 95768988 95770114 1127 - 1.776 2.319 1.820
ENSG00000077458 E004 146.2489764 0.0003205239 4.789796e-02 0.1054456534 11 95770115 95771525 1411 - 1.959 2.124 0.552
ENSG00000077458 E005 41.9461370 0.0037071580 1.748807e-02 0.0460179434 11 95771526 95771604 79 - 1.463 1.331 -0.459
ENSG00000077458 E006 46.4190552 0.0005195349 1.271761e-02 0.0352481023 11 95771605 95771650 46 - 1.504 1.386 -0.407
ENSG00000077458 E007 0.7406253 0.0154911323 6.583419e-01 0.7675172976 11 95775854 95775921 68 - 0.189 0.000 -10.083
ENSG00000077458 E008 60.1399142 0.0004314929 2.146552e-03 0.0076798522 11 95775922 95776023 102 - 1.616 1.479 -0.468
ENSG00000077458 E009 27.9917011 0.0006793608 3.881494e-02 0.0888410537 11 95778822 95778835 14 - 1.296 1.164 -0.466
ENSG00000077458 E010 57.1711075 0.0004299018 4.480000e-02 0.0998368016 11 95778836 95778954 119 - 1.587 1.531 -0.190
ENSG00000077458 E011 27.2317209 0.0007986000 1.612163e-01 0.2766831230 11 95778955 95778957 3 - 1.276 1.218 -0.205
ENSG00000077458 E012 5.1375116 0.0434067177 2.634846e-01 0.4013992068 11 95778958 95779077 120 - 0.653 0.469 -0.850
ENSG00000077458 E013 6.5903949 0.0387351818 6.292291e-01 0.7446391924 11 95779078 95779135 58 - 0.721 0.691 -0.124
ENSG00000077458 E014 3.0353238 0.0554358020 7.904107e-01 0.8659199845 11 95779136 95779219 84 - 0.472 0.469 -0.016
ENSG00000077458 E015 4.1864856 0.0113680239 3.523803e-01 0.4966487361 11 95779489 95779606 118 - 0.532 0.768 1.016
ENSG00000077458 E016 52.7261569 0.0004687037 4.708703e-01 0.6100527390 11 95779607 95779687 81 - 1.542 1.578 0.125
ENSG00000077458 E017 51.8760409 0.0004944345 3.912588e-02 0.0894198550 11 95779879 95779926 48 - 1.547 1.479 -0.235
ENSG00000077458 E018 61.3818702 0.0004529596 6.718895e-04 0.0027957446 11 95783065 95783197 133 - 1.627 1.465 -0.555
ENSG00000077458 E019 35.2515448 0.0010434283 7.260363e-05 0.0003926727 11 95783198 95783264 67 - 1.407 1.067 -1.201
ENSG00000077458 E020 0.4396707 0.0290589370 1.000000e+00 1.0000000000 11 95783265 95783268 4 - 0.123 0.000 -9.329
ENSG00000077458 E021 44.5612271 0.0040599057 2.236408e-02 0.0564373324 11 95786119 95786182 64 - 1.487 1.369 -0.408
ENSG00000077458 E022 52.4436259 0.0050649025 1.201029e-01 0.2201391077 11 95786183 95786274 92 - 1.547 1.507 -0.138
ENSG00000077458 E023 0.8814178 0.4170870410 7.720527e-01 0.8528839578 11 95786275 95786805 531 - 0.218 0.000 -9.400
ENSG00000077458 E024 25.3045481 0.0008032973 1.568797e-02 0.0420373173 11 95787624 95787624 1 - 1.258 1.067 -0.682
ENSG00000077458 E025 34.4811399 0.0005742484 1.552834e-01 0.2687726230 11 95787625 95787678 54 - 1.373 1.330 -0.148
ENSG00000077458 E026 32.9514943 0.0006904593 4.232433e-01 0.5663364903 11 95788499 95788563 65 - 1.326 1.479 0.529
ENSG00000077458 E027 2.8173112 0.2012715543 3.792628e-01 0.5235286064 11 95788564 95788858 295 - 0.384 0.705 1.518
ENSG00000077458 E028 1.4843564 0.0253552997 4.056952e-01 0.5493459862 11 95788859 95788868 10 - 0.247 0.470 1.350
ENSG00000077458 E029 1.9262173 0.0076725211 6.485373e-01 0.7600207695 11 95788869 95788947 79 - 0.320 0.469 0.832
ENSG00000077458 E030 1.9951174 0.0073288734 6.101754e-01 0.7295233757 11 95788948 95789391 444 - 0.363 0.295 -0.431
ENSG00000077458 E031 20.6595178 0.0050312758 6.976027e-01 0.7977016454 11 95789392 95789682 291 - 1.152 1.192 0.142
ENSG00000077458 E032 3.7654537 0.0042933291 5.036871e-01 0.6396563730 11 95789683 95789816 134 - 0.545 0.469 -0.370
ENSG00000077458 E033 13.0929742 0.0014240241 3.829008e-01 0.5270686648 11 95790156 95790422 267 - 0.983 0.943 -0.151