ENSG00000077254

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000370792 ENSG00000077254 HEK293_OSMI2_2hA HEK293_TMG_2hB USP33 protein_coding protein_coding 31.69747 7.229471 51.76799 0.3967307 1.430397 2.838383 3.310445 0.0131025 7.025358 0.01310250 0.6924294 8.2504314 0.06506667 0.00170000 0.13546667 0.133766667 6.452329e-17 6.452329e-17 FALSE TRUE
ENST00000370794 ENSG00000077254 HEK293_OSMI2_2hA HEK293_TMG_2hB USP33 protein_coding protein_coding 31.69747 7.229471 51.76799 0.3967307 1.430397 2.838383 13.114098 2.3016234 20.731972 0.18616229 2.1186495 3.1655748 0.37158750 0.32333333 0.40106667 0.077733333 5.881235e-01 6.452329e-17 FALSE TRUE
ENST00000481579 ENSG00000077254 HEK293_OSMI2_2hA HEK293_TMG_2hB USP33 protein_coding protein_coding 31.69747 7.229471 51.76799 0.3967307 1.430397 2.838383 2.584930 2.3732133 2.714900 0.82404861 0.4765366 0.1932955 0.14179583 0.31906667 0.05206667 -0.267000000 3.682116e-03 6.452329e-17 FALSE TRUE
ENST00000533928 ENSG00000077254 HEK293_OSMI2_2hA HEK293_TMG_2hB USP33 protein_coding retained_intron 31.69747 7.229471 51.76799 0.3967307 1.430397 2.838383 1.328467 0.2208261 1.817997 0.06586725 0.3058534 2.9853853 0.06150833 0.02966667 0.03530000 0.005633333 9.064799e-01 6.452329e-17   FALSE
MSTRG.1447.5 ENSG00000077254 HEK293_OSMI2_2hA HEK293_TMG_2hB USP33 protein_coding   31.69747 7.229471 51.76799 0.3967307 1.430397 2.838383 3.730261 0.6370325 7.312300 0.23734631 2.1557369 3.5003868 0.11888750 0.09053333 0.14026667 0.049733333 6.885947e-01 6.452329e-17 FALSE TRUE
MSTRG.1447.8 ENSG00000077254 HEK293_OSMI2_2hA HEK293_TMG_2hB USP33 protein_coding   31.69747 7.229471 51.76799 0.3967307 1.430397 2.838383 2.242074 0.0000000 2.732245 0.00000000 0.2334516 8.0992135 0.04362917 0.00000000 0.05263333 0.052633333 2.459056e-10 6.452329e-17 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000077254 E001 1.4757339 0.0447846514 4.196284e-04 1.849582e-03 1 77695987 77695990 4 - 0.130 0.810 3.973
ENSG00000077254 E002 46.1429362 0.0494827070 3.740658e-06 2.741524e-05 1 77695991 77696074 84 - 1.346 2.004 2.238
ENSG00000077254 E003 210.5151098 0.0359018557 1.299234e-06 1.053123e-05 1 77696075 77696321 247 - 2.032 2.583 1.841
ENSG00000077254 E004 516.1717801 0.0150292733 9.607473e-08 9.785521e-07 1 77696322 77697052 731 - 2.472 2.855 1.275
ENSG00000077254 E005 58.3703600 0.0013155262 5.674105e-06 3.988506e-05 1 77697053 77697074 22 - 1.549 1.880 1.120
ENSG00000077254 E006 264.3318160 0.0002226757 7.081138e-12 1.511720e-10 1 77697075 77697474 400 - 2.210 2.469 0.864
ENSG00000077254 E007 8.1936868 0.0024959048 6.962637e-01 7.966694e-01 1 77697609 77697862 254 - 0.817 0.812 -0.022
ENSG00000077254 E008 131.8551934 0.0002321543 1.140531e-02 3.213034e-02 1 77697863 77697931 69 - 1.928 2.098 0.570
ENSG00000077254 E009 165.3242770 0.0002489899 5.657006e-02 1.207104e-01 1 77701369 77701471 103 - 2.031 2.168 0.460
ENSG00000077254 E010 4.6198136 0.0157433268 5.655936e-01 6.926578e-01 1 77701472 77701651 180 - 0.591 0.746 0.657
ENSG00000077254 E011 0.2987644 0.0275349735 1.000000e+00   1 77711502 77711651 150 - 0.091 0.000 -10.248
ENSG00000077254 E012 0.1472490 0.0438825175 8.129592e-01   1 77711652 77711664 13 - 0.048 0.000 -9.218
ENSG00000077254 E013 5.4231100 0.0029791041 7.506196e-01 8.372437e-01 1 77711665 77711746 82 - 0.673 0.668 -0.022
ENSG00000077254 E014 123.7670828 0.0002674027 2.856409e-02 6.913940e-02 1 77711747 77711784 38 - 1.902 2.062 0.535
ENSG00000077254 E015 147.3839989 0.0003735832 2.891863e-01 4.298485e-01 1 77711785 77711855 71 - 1.986 2.095 0.364
ENSG00000077254 E016 150.6560549 0.0023648151 9.893251e-01 9.973324e-01 1 77713200 77713281 82 - 2.003 2.069 0.224
ENSG00000077254 E017 0.4386386 0.1457216413 3.403891e-01 4.843567e-01 1 77713282 77713508 227 - 0.090 0.280 1.969
ENSG00000077254 E018 218.8666054 0.0097520360 5.553879e-01 6.842553e-01 1 77714614 77714783 170 - 2.157 2.264 0.358
ENSG00000077254 E019 0.8899506 0.0139212120 4.631335e-01 6.029609e-01 1 77714784 77714917 134 - 0.229 0.000 -11.805
ENSG00000077254 E020 184.2879575 0.0002203816 8.049249e-01 8.758775e-01 1 77715742 77715868 127 - 2.088 2.163 0.249
ENSG00000077254 E021 0.0000000       1 77717540 77717644 105 -      
ENSG00000077254 E022 0.2924217 0.0272395576 1.000000e+00   1 77717645 77717777 133 - 0.091 0.000 -10.248
ENSG00000077254 E023 0.7427016 0.0157316954 5.957522e-01 7.178004e-01 1 77717778 77717866 89 - 0.199 0.000 -11.552
ENSG00000077254 E024 224.4967003 0.0014567552 1.497315e-01 2.613118e-01 1 77717867 77718023 157 - 2.167 2.286 0.397
ENSG00000077254 E025 108.7938395 0.0058290645 4.827171e-02 1.061158e-01 1 77718024 77718047 24 - 1.843 2.024 0.608
ENSG00000077254 E026 128.2491513 0.0053190678 3.253464e-01 4.687853e-01 1 77718596 77718641 46 - 1.925 2.045 0.403
ENSG00000077254 E027 0.5202097 0.0208120415 3.271760e-01 4.706198e-01 1 77718642 77718712 71 - 0.091 0.282 1.980
ENSG00000077254 E028 106.7897745 0.0002749367 7.621961e-02 1.534542e-01 1 77721172 77721205 34 - 1.868 1.842 -0.087
ENSG00000077254 E029 6.1350520 0.0398933268 2.495743e-01 3.854153e-01 1 77721206 77721738 533 - 0.672 0.920 0.981
ENSG00000077254 E030 138.2926719 0.0002263972 1.793922e-02 4.700531e-02 1 77721831 77721901 71 - 1.981 1.939 -0.143
ENSG00000077254 E031 58.7793616 0.0004484178 1.624745e-01 2.783684e-01 1 77721902 77721925 24 - 1.616 1.585 -0.107
ENSG00000077254 E032 204.0389429 0.0002161542 6.725225e-07 5.794455e-06 1 77722024 77722196 173 - 2.160 2.029 -0.437
ENSG00000077254 E033 8.3135017 0.0019872286 6.611585e-01 7.696696e-01 1 77722197 77722399 203 - 0.825 0.812 -0.052
ENSG00000077254 E034 203.2352892 0.0001780814 7.161588e-08 7.477219e-07 1 77723331 77723443 113 - 2.160 2.010 -0.500
ENSG00000077254 E035 213.0774162 0.0002440399 1.987063e-08 2.318285e-07 1 77725622 77725762 141 - 2.181 2.026 -0.519
ENSG00000077254 E036 349.9692150 0.0001790482 5.561819e-15 1.901931e-13 1 77728295 77728712 418 - 2.397 2.221 -0.587
ENSG00000077254 E037 117.4585549 0.0008851540 1.391529e-04 6.998541e-04 1 77729860 77729933 74 - 1.922 1.781 -0.474
ENSG00000077254 E038 66.8241559 0.0407501537 2.124621e-01 3.414429e-01 1 77729934 77729938 5 - 1.674 1.597 -0.259
ENSG00000077254 E039 140.8559334 0.0002400136 8.836019e-06 5.947408e-05 1 77730618 77730731 114 - 2.001 1.853 -0.496
ENSG00000077254 E040 73.5149685 0.0003634761 1.110085e-01 2.070271e-01 1 77734347 77734356 10 - 1.711 1.678 -0.114
ENSG00000077254 E041 88.9946752 0.0003076636 2.533777e-02 6.256895e-02 1 77734357 77734416 60 - 1.795 1.732 -0.213
ENSG00000077254 E042 162.3814267 0.0047133894 9.287827e-05 4.885335e-04 1 77736056 77736158 103 - 2.064 1.906 -0.530
ENSG00000077254 E043 128.5408397 0.0002331194 2.983120e-06 2.233414e-05 1 77739265 77739310 46 - 1.965 1.794 -0.578
ENSG00000077254 E044 112.2057985 0.0002730903 1.574463e-04 7.803404e-04 1 77739311 77739330 20 - 1.903 1.768 -0.458
ENSG00000077254 E045 118.1949302 0.0003812509 5.914341e-06 4.142006e-05 1 77739331 77739376 46 - 1.930 1.754 -0.595
ENSG00000077254 E046 98.9755130 0.0021053723 3.141457e-06 2.339661e-05 1 77739377 77739397 21 - 1.859 1.624 -0.794
ENSG00000077254 E047 87.4190060 0.0050326370 3.214978e-04 1.466962e-03 1 77739398 77739417 20 - 1.803 1.605 -0.668
ENSG00000077254 E048 110.6257819 0.0320160685 1.460521e-02 3.960500e-02 1 77740877 77740934 58 - 1.902 1.712 -0.642
ENSG00000077254 E049 62.3607210 0.0416969001 2.950186e-02 7.097192e-02 1 77740935 77740939 5 - 1.660 1.453 -0.708
ENSG00000077254 E050 106.9487089 0.0007791107 5.236367e-08 5.623190e-07 1 77741376 77741429 54 - 1.894 1.634 -0.879
ENSG00000077254 E051 145.6484529 0.0181389761 4.375978e-05 2.500199e-04 1 77741617 77741748 132 - 2.028 1.743 -0.957
ENSG00000077254 E052 56.4602589 0.0075215437 9.746277e-06 6.497987e-05 1 77745421 77745599 179 - 1.628 1.265 -1.253
ENSG00000077254 E053 0.0000000       1 77745600 77745638 39 -      
ENSG00000077254 E054 1.1888288 0.0119618579 3.011327e-01 4.429039e-01 1 77746399 77746556 158 - 0.285 0.000 -12.203
ENSG00000077254 E055 0.1451727 0.0428346089 8.107156e-01   1 77759348 77759642 295 - 0.048 0.000 -9.221
ENSG00000077254 E056 82.8254472 0.0345435829 1.210589e-03 4.676353e-03 1 77759643 77759700 58 - 1.788 1.472 -1.074
ENSG00000077254 E057 72.4359980 0.0319926894 3.898277e-03 1.285853e-02 1 77759701 77759852 152 - 1.728 1.458 -0.921