ENSG00000077150

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000189444 ENSG00000077150 HEK293_OSMI2_2hA HEK293_TMG_2hB NFKB2 protein_coding protein_coding 12.34028 19.50379 7.220501 1.462115 0.482941 -1.432327 1.4184442 3.5720854 0.0000000 0.6550092 0.00000000 -8.4846559 0.07906250 0.19076667 0.00000000 -0.19076667 1.734241e-10 1.734241e-10 FALSE TRUE
ENST00000336486 ENSG00000077150 HEK293_OSMI2_2hA HEK293_TMG_2hB NFKB2 protein_coding processed_transcript 12.34028 19.50379 7.220501 1.462115 0.482941 -1.432327 0.7527939 0.7106890 0.6144784 0.1770079 0.14154778 -0.2067250 0.06350417 0.03543333 0.08333333 0.04790000 4.636256e-02 1.734241e-10 FALSE TRUE
ENST00000428099 ENSG00000077150 HEK293_OSMI2_2hA HEK293_TMG_2hB NFKB2 protein_coding protein_coding 12.34028 19.50379 7.220501 1.462115 0.482941 -1.432327 0.8613953 0.9371176 0.7544140 0.5330206 0.20662593 -0.3091893 0.07064583 0.04680000 0.10706667 0.06026667 3.197000e-01 1.734241e-10 FALSE TRUE
ENST00000467116 ENSG00000077150 HEK293_OSMI2_2hA HEK293_TMG_2hB NFKB2 protein_coding retained_intron 12.34028 19.50379 7.220501 1.462115 0.482941 -1.432327 0.4568012 0.2598162 0.3284000 0.1065770 0.08888496 0.3267528 0.05370000 0.01260000 0.04443333 0.03183333 8.045862e-02 1.734241e-10 FALSE TRUE
ENST00000652277 ENSG00000077150 HEK293_OSMI2_2hA HEK293_TMG_2hB NFKB2 protein_coding protein_coding 12.34028 19.50379 7.220501 1.462115 0.482941 -1.432327 3.1474893 4.3892539 1.6671898 0.6328863 0.13007384 -1.3912129 0.27524583 0.22310000 0.23346667 0.01036667 9.494290e-01 1.734241e-10 FALSE TRUE
ENST00000661543 ENSG00000077150 HEK293_OSMI2_2hA HEK293_TMG_2hB NFKB2 protein_coding protein_coding 12.34028 19.50379 7.220501 1.462115 0.482941 -1.432327 4.6065642 7.5473792 3.2468697 1.1904489 0.47639548 -1.2144000 0.36517500 0.38190000 0.45030000 0.06840000 7.204623e-01 1.734241e-10 FALSE TRUE
MSTRG.4544.3 ENSG00000077150 HEK293_OSMI2_2hA HEK293_TMG_2hB NFKB2 protein_coding   12.34028 19.50379 7.220501 1.462115 0.482941 -1.432327 0.3224173 1.2240793 0.0000000 0.6120646 0.00000000 -6.9472913 0.02121250 0.06516667 0.00000000 -0.06516667 2.673840e-01 1.734241e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000077150 E001 4.7428056 0.0946679566 0.1181416004 0.2172715001 10 102394110 102394328 219 + 0.876 0.610 -1.083
ENSG00000077150 E002 7.5922142 0.1246423948 0.5580868969 0.6863798202 10 102394329 102394502 174 + 0.941 0.820 -0.461
ENSG00000077150 E003 7.5104014 0.0023423515 0.7205377285 0.8151373218 10 102394503 102394555 53 + 0.875 0.839 -0.140
ENSG00000077150 E004 8.9464937 0.0019091491 0.9474037668 0.9709383106 10 102394556 102394600 45 + 0.908 0.912 0.013
ENSG00000077150 E005 1.6198729 0.0088761513 0.3746238040 0.5190544701 10 102394601 102394774 174 + 0.202 0.386 1.276
ENSG00000077150 E006 3.2355813 0.0060113563 0.9601189555 0.9789089159 10 102395693 102395704 12 + 0.527 0.545 0.085
ENSG00000077150 E007 3.2355813 0.0060113563 0.9601189555 0.9789089159 10 102395705 102395705 1 + 0.527 0.545 0.085
ENSG00000077150 E008 3.2355813 0.0060113563 0.9601189555 0.9789089159 10 102395706 102395706 1 + 0.527 0.545 0.085
ENSG00000077150 E009 14.5928889 0.0018331858 0.0116389705 0.0326739380 10 102395707 102395796 90 + 1.273 1.043 -0.823
ENSG00000077150 E010 12.8300424 0.0014313946 0.1662265395 0.2832903261 10 102395797 102395887 91 + 1.147 1.013 -0.483
ENSG00000077150 E011 53.7190230 0.0004346982 0.0264605045 0.0648704394 10 102395888 102395980 93 + 1.725 1.616 -0.370
ENSG00000077150 E012 73.3710484 0.0003827180 0.1803312516 0.3015873746 10 102396253 102396334 82 + 1.812 1.760 -0.175
ENSG00000077150 E013 67.9896322 0.0014481804 0.6176916389 0.7354482623 10 102396449 102396489 41 + 1.753 1.740 -0.045
ENSG00000077150 E014 1.4832105 0.0923616963 0.0134838643 0.0370445007 10 102396490 102396724 235 + 0.658 0.188 -2.715
ENSG00000077150 E015 88.7409258 0.0058929397 0.7509440740 0.8375086980 10 102396725 102396823 99 + 1.861 1.856 -0.017
ENSG00000077150 E016 129.6866139 0.0006677734 0.0477297687 0.1051413529 10 102396904 102397055 152 + 2.066 2.005 -0.204
ENSG00000077150 E017 64.9736835 0.0003982194 0.0002197673 0.0010486159 10 102397302 102397341 40 + 1.850 1.682 -0.568
ENSG00000077150 E018 60.5485519 0.0004079560 0.0184117342 0.0480361342 10 102397342 102397373 32 + 1.775 1.666 -0.370
ENSG00000077150 E019 61.5770471 0.0005761586 0.1188780529 0.2183376361 10 102397374 102397408 35 + 1.748 1.679 -0.234
ENSG00000077150 E020 135.1718714 0.0002550050 0.0243088082 0.0604527599 10 102397527 102397685 159 + 2.085 2.019 -0.223
ENSG00000077150 E021 137.9506863 0.0002578865 0.0021010438 0.0075371416 10 102397981 102398072 92 + 2.114 2.020 -0.314
ENSG00000077150 E022 89.1257384 0.0003305123 0.0021159656 0.0075842083 10 102398073 102398085 13 + 1.947 1.829 -0.398
ENSG00000077150 E023 128.6684652 0.0002562932 0.0353500804 0.0823238969 10 102398212 102398297 86 + 2.061 1.998 -0.213
ENSG00000077150 E024 126.7757687 0.0002716241 0.5448136004 0.6752322588 10 102398385 102398523 139 + 1.975 2.012 0.123
ENSG00000077150 E025 93.0192650 0.0062052170 0.5573866756 0.6858035729 10 102398739 102398864 126 + 1.835 1.884 0.164
ENSG00000077150 E026 2.8283939 0.0324672247 0.2142684716 0.3435734768 10 102398865 102399218 354 + 0.658 0.448 -0.975
ENSG00000077150 E027 75.0343697 0.0115505603 0.5591604158 0.6872985479 10 102399288 102399497 210 + 1.800 1.776 -0.081
ENSG00000077150 E028 81.3972201 0.0133851955 0.0382287832 0.0877783496 10 102399577 102399718 142 + 1.911 1.787 -0.417
ENSG00000077150 E029 14.4436676 0.0013501446 0.0001818282 0.0008857955 10 102399927 102400079 153 + 1.337 0.998 -1.209
ENSG00000077150 E030 102.4522464 0.0009248632 0.0165763817 0.0440089379 10 102400080 102400194 115 + 1.981 1.893 -0.294
ENSG00000077150 E031 137.7254389 0.0011367808 0.7605128493 0.8446107847 10 102400278 102400491 214 + 2.019 2.046 0.089
ENSG00000077150 E032 8.1382236 0.0036653669 0.0057239008 0.0178824822 10 102400578 102400654 77 + 1.108 0.792 -1.188
ENSG00000077150 E033 156.1535703 0.0057321415 0.0454919134 0.1010968763 10 102400655 102400824 170 + 2.001 2.120 0.396
ENSG00000077150 E034 138.6926268 0.0024894718 0.0076546307 0.0229130943 10 102400947 102401049 103 + 1.935 2.071 0.454
ENSG00000077150 E035 0.6234634 0.0192090947 0.9139064488 0.9494959781 10 102401050 102401179 130 + 0.202 0.186 -0.143
ENSG00000077150 E036 173.6488561 0.0002090484 0.0048818312 0.0156010910 10 102401180 102401331 152 + 2.052 2.161 0.363
ENSG00000077150 E037 103.5542548 0.0003782112 0.0084318044 0.0248654293 10 102401449 102401518 70 + 1.812 1.943 0.440
ENSG00000077150 E038 144.3961004 0.0101040956 0.0021760353 0.0077682098 10 102401745 102401917 173 + 1.895 2.099 0.684
ENSG00000077150 E039 101.0543640 0.0101552118 0.0013562521 0.0051638542 10 102402048 102402104 57 + 1.715 1.950 0.790
ENSG00000077150 E040 112.7171968 0.0095106846 0.0006057670 0.0025543825 10 102402105 102402159 55 + 1.758 1.997 0.807
ENSG00000077150 E041 35.7609210 0.0006455637 0.0349358118 0.0815335354 10 102402252 102402254 3 + 1.325 1.497 0.598
ENSG00000077150 E042 110.2451291 0.0099495152 0.0018514935 0.0067630704 10 102402255 102402524 270 + 1.762 1.986 0.755