ENSG00000076641

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000220597 ENSG00000076641 HEK293_OSMI2_2hA HEK293_TMG_2hB PAG1 protein_coding protein_coding 3.199093 1.055671 4.779101 0.1328172 0.2475316 2.167993 2.4773659 0.87270189 3.4588386 0.08591219 0.07686205 1.974455 0.81746250 0.8334667 0.72820000 -0.10526667 0.51412831 0.02217737 FALSE  
MSTRG.31725.4 ENSG00000076641 HEK293_OSMI2_2hA HEK293_TMG_2hB PAG1 protein_coding   3.199093 1.055671 4.779101 0.1328172 0.2475316 2.167993 0.2420458 0.00000000 0.3930687 0.00000000 0.11227450 5.332954 0.06217917 0.0000000 0.08410000 0.08410000 0.02217737 0.02217737 TRUE  
MSTRG.31725.5 ENSG00000076641 HEK293_OSMI2_2hA HEK293_TMG_2hB PAG1 protein_coding   3.199093 1.055671 4.779101 0.1328172 0.2475316 2.167993 0.1952602 0.03326222 0.4061374 0.02517324 0.09943976 3.265880 0.05116667 0.0374000 0.08666667 0.04926667 0.57692956 0.02217737 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000076641 E001 156.501079 0.0012768336 4.711150e-12 1.034438e-10 8 80967810 80970290 2481 - 2.075 2.284 0.700
ENSG00000076641 E002 38.297484 0.0030338868 1.070946e-01 2.012494e-01 8 80970291 80970811 521 - 1.563 1.450 -0.389
ENSG00000076641 E003 13.333676 0.0384364578 5.328193e-01 6.651315e-01 8 80970812 80970879 68 - 1.077 1.163 0.312
ENSG00000076641 E004 10.042077 0.1018203637 6.551640e-01 7.651126e-01 8 80970880 80970907 28 - 0.995 0.936 -0.222
ENSG00000076641 E005 241.590288 0.0019930020 3.272994e-01 4.707367e-01 8 80970908 80975521 4614 - 2.332 2.307 -0.085
ENSG00000076641 E006 108.861860 0.0078716451 4.865336e-01 6.242538e-01 8 80975522 80976906 1385 - 1.990 1.948 -0.139
ENSG00000076641 E007 14.841306 0.0145269291 2.047971e-01 3.321393e-01 8 80980435 80980494 60 - 1.172 1.014 -0.573
ENSG00000076641 E008 46.442478 0.0008252332 9.179032e-02 1.779276e-01 8 80984776 80985227 452 - 1.640 1.537 -0.352
ENSG00000076641 E009 21.920187 0.0010872457 5.203841e-02 1.127819e-01 8 80985228 80985377 150 - 1.340 1.165 -0.618
ENSG00000076641 E010 18.251057 0.0022067796 1.127727e-01 2.095909e-01 8 80987370 80987466 97 - 1.262 1.106 -0.557
ENSG00000076641 E011 0.000000       8 80987467 80987534 68 -      
ENSG00000076641 E012 14.889556 0.0012188118 7.970573e-01 8.704983e-01 8 80991479 80991530 52 - 1.152 1.126 -0.092
ENSG00000076641 E013 25.622203 0.0007777612 1.018056e-01 1.932522e-01 8 80993103 80993307 205 - 1.397 1.263 -0.469
ENSG00000076641 E014 2.871742 0.0110352506 2.094257e-01 3.378499e-01 8 80994939 80995040 102 - 0.592 0.350 -1.235
ENSG00000076641 E015 0.000000       8 81029791 81029995 205 -      
ENSG00000076641 E016 13.860387 0.0458766396 2.289775e-01 3.612186e-01 8 81029996 81030089 94 - 1.157 0.956 -0.733
ENSG00000076641 E017 8.126156 0.0552100885 2.505835e-01 3.866326e-01 8 81070112 81070170 59 - 0.948 0.729 -0.853
ENSG00000076641 E018 0.000000       8 81070171 81070176 6 -      
ENSG00000076641 E019 16.249452 0.0893253245 8.878672e-01 9.323159e-01 8 81111591 81112068 478 - 1.182 1.155 -0.099