ENSG00000075618

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000382361 ENSG00000075618 HEK293_OSMI2_2hA HEK293_TMG_2hB FSCN1 protein_coding protein_coding 27.47313 50.43255 15.78791 3.536824 1.149901 -1.674907 21.777470 44.021370 10.583084 4.007746 0.151382 -2.0554091 0.7561500 0.8706000 0.6767000 -0.1939000 0.015277764 0.007330513 FALSE TRUE
ENST00000405801 ENSG00000075618 HEK293_OSMI2_2hA HEK293_TMG_2hB FSCN1 protein_coding protein_coding 27.47313 50.43255 15.78791 3.536824 1.149901 -1.674907 5.506704 6.050908 5.073093 1.147497 1.061905 -0.2538273 0.2373083 0.1215667 0.3153333 0.1937667 0.007330513 0.007330513 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000075618 E001 110.2331478 0.0017288949 1.166261e-01 2.151422e-01 7 5592816 5592865 50 + 1.943 1.912 -0.103
ENSG00000075618 E002 906.4108449 0.0095936665 4.167367e-03 1.361269e-02 7 5592866 5593768 903 + 2.889 2.824 -0.214
ENSG00000075618 E003 0.1482932 0.0418245058 1.000000e+00   7 5593848 5594027 180 + 0.001 0.066 6.397
ENSG00000075618 E004 0.4762024 0.1501402144 9.181685e-02 1.779627e-01 7 5594547 5594685 139 + 0.360 0.066 -2.978
ENSG00000075618 E005 0.4804688 0.0221184819 7.046667e-02 1.441123e-01 7 5594686 5594820 135 + 0.360 0.066 -2.981
ENSG00000075618 E006 0.3332198 0.0284427291 2.867138e-01   7 5594821 5594896 76 + 0.217 0.066 -1.981
ENSG00000075618 E007 2.0972213 0.0071662303 2.359870e-01 3.695254e-01 7 5602872 5603256 385 + 0.555 0.364 -0.980
ENSG00000075618 E008 540.6721648 0.0012678595 1.135978e-03 4.424499e-03 7 5603257 5603413 157 + 2.637 2.603 -0.113
ENSG00000075618 E009 516.4992781 0.0002605767 7.977037e-03 2.371964e-02 7 5603496 5603617 122 + 2.599 2.587 -0.041
ENSG00000075618 E010 211.3256715 0.0002185625 2.902566e-04 1.340261e-03 7 5603863 5603871 9 + 2.257 2.188 -0.232
ENSG00000075618 E011 548.9085160 0.0001178050 2.916465e-01 4.325245e-01 7 5603872 5604030 159 + 2.597 2.618 0.068
ENSG00000075618 E012 2187.4696873 0.0055091846 8.023098e-07 6.799921e-06 7 5605272 5608281 3010 + 3.079 3.243 0.546