ENSG00000075391

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000367649 ENSG00000075391 HEK293_OSMI2_2hA HEK293_TMG_2hB RASAL2 protein_coding protein_coding 3.603472 1.894699 5.077364 0.08275773 0.08542579 1.417355 0.8228677 0.2976558 1.716401 0.10645950 0.08653918 2.4883783 0.2494917 0.1527667 0.3378667 0.18510000 6.167055e-02 8.281245e-09 FALSE TRUE
ENST00000463079 ENSG00000075391 HEK293_OSMI2_2hA HEK293_TMG_2hB RASAL2 protein_coding retained_intron 3.603472 1.894699 5.077364 0.08275773 0.08542579 1.417355 1.2046300 0.6872655 1.663324 0.06745017 0.13098561 1.2629365 0.3448833 0.3632000 0.3269333 -0.03626667 8.002423e-01 8.281245e-09 FALSE FALSE
ENST00000696606 ENSG00000075391 HEK293_OSMI2_2hA HEK293_TMG_2hB RASAL2 protein_coding protein_coding 3.603472 1.894699 5.077364 0.08275773 0.08542579 1.417355 0.7086495 0.0000000 1.094219 0.00000000 0.12160824 6.7868819 0.1351917 0.0000000 0.2164000 0.21640000 8.281245e-09 8.281245e-09 FALSE TRUE
MSTRG.2646.1 ENSG00000075391 HEK293_OSMI2_2hA HEK293_TMG_2hB RASAL2 protein_coding   3.603472 1.894699 5.077364 0.08275773 0.08542579 1.417355 0.7751587 0.8228288 0.567188 0.05610541 0.16232651 -0.5289787 0.2338542 0.4383333 0.1118333 -0.32650000 2.500135e-04 8.281245e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000075391 E001 5.0520564 0.0043717457 5.778260e-01 7.030075e-01 1 178094103 178094103 1 + 0.736 0.837 0.401
ENSG00000075391 E002 13.8107934 0.0223713922 9.159751e-01 9.508702e-01 1 178094104 178094323 220 + 1.142 1.189 0.168
ENSG00000075391 E003 21.7382826 0.0009021185 4.195895e-02 9.464895e-02 1 178094324 178094694 371 + 1.388 1.260 -0.446
ENSG00000075391 E004 0.1515154 0.0425099807 8.503631e-01   1 178120961 178121087 127 + 0.091 0.000 -11.042
ENSG00000075391 E005 0.1515154 0.0425099807 8.503631e-01   1 178123056 178123182 127 + 0.091 0.000 -11.042
ENSG00000075391 E006 0.1451727 0.0431941288 8.504119e-01   1 178123719 178123815 97 + 0.091 0.000 -11.036
ENSG00000075391 E007 0.1451727 0.0431941288 8.504119e-01   1 178125327 178125501 175 + 0.091 0.000 -11.036
ENSG00000075391 E008 0.0000000       1 178230310 178233880 3571 +      
ENSG00000075391 E009 15.7510484 0.0011941985 5.581733e-02 1.193779e-01 1 178283564 178283691 128 + 1.263 1.121 -0.505
ENSG00000075391 E010 19.5060187 0.0010931580 7.864301e-03 2.343997e-02 1 178299992 178300118 127 + 1.366 1.177 -0.666
ENSG00000075391 E011 0.0000000       1 178341471 178341608 138 +      
ENSG00000075391 E012 0.0000000       1 178371374 178371475 102 +      
ENSG00000075391 E013 0.0000000       1 178372203 178372322 120 +      
ENSG00000075391 E014 0.1817044 0.0396677339 2.748583e-01   1 178389010 178389307 298 + 0.000 0.162 11.483
ENSG00000075391 E015 18.2521007 0.0220928186 3.979249e-01 5.416688e-01 1 178390100 178390206 107 + 1.300 1.236 -0.224
ENSG00000075391 E016 20.9078657 0.0487993658 6.926913e-01 7.938628e-01 1 178420511 178420620 110 + 1.334 1.333 -0.005
ENSG00000075391 E017 25.1780001 0.0139381983 2.648515e-01 4.028489e-01 1 178439422 178439575 154 + 1.435 1.371 -0.222
ENSG00000075391 E018 20.0169150 0.0014066932 1.022959e-02 2.932978e-02 1 178441549 178441647 99 + 1.371 1.190 -0.635
ENSG00000075391 E019 61.0011180 0.0004667795 1.707721e-07 1.659005e-06 1 178442675 178443229 555 + 1.851 1.630 -0.751
ENSG00000075391 E020 34.4219564 0.0080468938 5.748322e-02 1.223159e-01 1 178445518 178445662 145 + 1.577 1.464 -0.389
ENSG00000075391 E021 29.3504961 0.0149958029 5.097761e-02 1.109061e-01 1 178451571 178451715 145 + 1.523 1.380 -0.491
ENSG00000075391 E022 12.1344706 0.0159911327 3.230554e-01 4.663932e-01 1 178452416 178452436 21 + 1.143 1.055 -0.315
ENSG00000075391 E023 31.2658580 0.0006944137 4.171373e-01 5.605235e-01 1 178452437 178452652 216 + 1.510 1.489 -0.073
ENSG00000075391 E024 28.7155997 0.0092002906 3.809383e-01 5.252221e-01 1 178454447 178454648 202 + 1.424 1.524 0.345
ENSG00000075391 E025 1.7305929 0.0099647671 9.677074e-01 9.838217e-01 1 178456475 178456699 225 + 0.421 0.448 0.142
ENSG00000075391 E026 6.8621403 0.0024500364 2.992969e-02 7.182492e-02 1 178456700 178456720 21 + 0.755 1.039 1.086
ENSG00000075391 E027 34.8617610 0.0024157938 4.732888e-01 6.122692e-01 1 178456721 178456899 179 + 1.552 1.537 -0.053
ENSG00000075391 E028 95.4758952 0.0018004383 6.820880e-01 7.856084e-01 1 178457683 178458544 862 + 1.966 1.980 0.047
ENSG00000075391 E029 28.0287591 0.0044194144 4.781193e-01 6.165730e-01 1 178464278 178464412 135 + 1.416 1.496 0.276
ENSG00000075391 E030 31.8938910 0.0193977754 5.365644e-02 1.156513e-01 1 178465920 178466122 203 + 1.408 1.613 0.702
ENSG00000075391 E031 18.8816445 0.0072547141 2.219105e-01 3.526780e-01 1 178467334 178467421 88 + 1.223 1.358 0.473
ENSG00000075391 E032 0.0000000       1 178470693 178470723 31 +      
ENSG00000075391 E033 425.3481865 0.0039987313 1.625494e-04 8.029646e-04 1 178473075 178484147 11073 + 2.569 2.685 0.386