ENSG00000075336

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000169551 ENSG00000075336 HEK293_OSMI2_2hA HEK293_TMG_2hB TIMM21 protein_coding protein_coding 22.07729 10.74864 31.24757 0.5675599 0.5188527 1.53871 5.792336 3.875785 7.051483 0.5055029 0.4174509 0.861765 0.2730417 0.3578000 0.2262000 -0.13160000 0.01547236 0.01547236 FALSE TRUE
ENST00000577952 ENSG00000075336 HEK293_OSMI2_2hA HEK293_TMG_2hB TIMM21 protein_coding retained_intron 22.07729 10.74864 31.24757 0.5675599 0.5188527 1.53871 10.872110 5.044556 15.127218 0.8877234 0.9018251 1.582444 0.4841792 0.4636000 0.4835333 0.01993333 0.93455618 0.01547236 TRUE FALSE
ENST00000581467 ENSG00000075336 HEK293_OSMI2_2hA HEK293_TMG_2hB TIMM21 protein_coding retained_intron 22.07729 10.74864 31.24757 0.5675599 0.5188527 1.53871 4.715988 1.481163 8.139144 0.7521289 0.4952463 2.450211 0.2051500 0.1456667 0.2605667 0.11490000 0.69078949 0.01547236 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000075336 E001 9.310388 0.0180775560 1.454111e-01 0.2554141871 18 74148523 74148537 15 + 1.074 0.886 -0.697
ENSG00000075336 E002 186.798671 0.0013883313 4.954302e-02 0.1083532702 18 74148538 74148781 244 + 2.289 2.234 -0.184
ENSG00000075336 E003 281.516728 0.0004243556 9.485488e-05 0.0004978245 18 74148782 74149109 328 + 2.474 2.392 -0.272
ENSG00000075336 E004 1.819653 0.2402145953 7.935517e-01 0.8680007264 18 74155095 74155144 50 + 0.462 0.378 -0.451
ENSG00000075336 E005 105.444620 0.0003656265 4.710985e-02 0.1040225225 18 74155145 74155174 30 + 2.043 1.974 -0.230
ENSG00000075336 E006 104.747391 0.0003046729 8.554481e-01 0.9106043545 18 74155175 74155207 33 + 2.011 2.019 0.028
ENSG00000075336 E007 10.060369 0.0018087586 7.498369e-01 0.8366719406 18 74155208 74155305 98 + 1.019 1.054 0.127
ENSG00000075336 E008 167.305280 0.0016371639 3.889024e-01 0.5329792429 18 74155306 74155403 98 + 2.205 2.233 0.094
ENSG00000075336 E009 1.807081 0.0282908062 6.356156e-01 0.7498696659 18 74155404 74155493 90 + 0.461 0.371 -0.484
ENSG00000075336 E010 12.135366 0.0019418834 1.231789e-02 0.0342967689 18 74156237 74158012 1776 + 1.194 0.935 -0.943
ENSG00000075336 E011 142.161065 0.0003849343 2.437143e-01 0.3785466043 18 74158014 74158087 74 + 2.132 2.169 0.122
ENSG00000075336 E012 159.313155 0.0002243720 3.464659e-02 0.0809802508 18 74158171 74158276 106 + 2.173 2.234 0.201
ENSG00000075336 E013 9.819442 0.0086362372 4.621789e-01 0.6020465596 18 74158277 74158375 99 + 0.989 1.071 0.301
ENSG00000075336 E014 233.748866 0.0011736852 3.795530e-05 0.0002200146 18 74158376 74160531 2156 + 2.318 2.424 0.354