ENSG00000075223

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000265361 ENSG00000075223 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA3C protein_coding protein_coding 1.27988 0.3858491 1.976664 0.1408271 0.173572 2.327325 0.76924686 0.2438124 1.1137231 0.002356257 0.09432185 2.146452 0.72864583 0.7872 0.5787333 -0.2084667 0.5438780104 0.0003665092 FALSE TRUE
ENST00000411788 ENSG00000075223 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA3C protein_coding nonsense_mediated_decay 1.27988 0.3858491 1.976664 0.1408271 0.173572 2.327325 0.03478691 0.1420367 0.0000000 0.142036672 0.00000000 -3.926347 0.05127917 0.2128 0.0000000 -0.2128000 0.4014765257 0.0003665092   FALSE
ENST00000419255 ENSG00000075223 HEK293_OSMI2_2hA HEK293_TMG_2hB SEMA3C protein_coding protein_coding 1.27988 0.3858491 1.976664 0.1408271 0.173572 2.327325 0.41864168 0.0000000 0.6915497 0.000000000 0.14812631 6.132473 0.19737500 0.0000 0.3421333 0.3421333 0.0003665092 0.0003665092 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000075223 E001 40.6375251 0.002410563 5.474125e-13 1.399914e-11 7 80742538 80744867 2330 - 1.399 1.800 1.370
ENSG00000075223 E002 16.9745706 0.001482432 1.126712e-02 3.180111e-02 7 80744868 80745307 440 - 1.175 0.868 -1.129
ENSG00000075223 E003 7.6082517 0.002213020 3.075631e-01 4.497988e-01 7 80748898 80749028 131 - 0.852 0.698 -0.617
ENSG00000075223 E004 5.4552510 0.019707836 8.621354e-02 1.693413e-01 7 80751269 80751336 68 - 0.752 0.413 -1.547
ENSG00000075223 E005 6.5859156 0.002563850 1.080276e-01 2.026149e-01 7 80758331 80758488 158 - 0.809 0.530 -1.188
ENSG00000075223 E006 2.7325336 0.005842289 6.708388e-01 7.771929e-01 7 80761616 80761657 42 - 0.495 0.414 -0.415
ENSG00000075223 E007 5.1056973 0.003638890 1.093734e-01 2.046384e-01 7 80765155 80765243 89 - 0.720 0.414 -1.415
ENSG00000075223 E008 10.3205281 0.004742981 5.664684e-03 1.772613e-02 7 80789306 80789528 223 - 0.990 0.530 -1.876
ENSG00000075223 E009 0.0000000       7 80793357 80793493 137 -      
ENSG00000075223 E010 0.0000000       7 80796389 80796624 236 -      
ENSG00000075223 E011 7.6485999 0.004032086 4.952378e-02 1.083237e-01 7 80798092 80798236 145 - 0.868 0.530 -1.416
ENSG00000075223 E012 0.0000000       7 80798237 80798238 2 -      
ENSG00000075223 E013 4.4686419 0.029466530 2.001971e-01 3.264902e-01 7 80800757 80800826 70 - 0.674 0.414 -1.224
ENSG00000075223 E014 7.1353707 0.002570162 2.065510e-01 3.344056e-01 7 80802665 80802779 115 - 0.827 0.622 -0.841
ENSG00000075223 E015 8.6991227 0.002076374 1.833790e-01 3.054468e-01 7 80804106 80804248 143 - 0.898 0.698 -0.793
ENSG00000075223 E016 8.2957431 0.025178055 5.147330e-01 6.494696e-01 7 80805639 80805758 120 - 0.868 0.764 -0.410
ENSG00000075223 E017 7.2478471 0.002790792 9.908145e-01 9.984300e-01 7 80810611 80810701 91 - 0.809 0.818 0.034
ENSG00000075223 E018 0.3268771 0.029178012 1.976852e-01   7 80815949 80816224 276 - 0.054 0.255 2.586
ENSG00000075223 E019 6.5157547 0.002820850 7.460177e-01 8.338888e-01 7 80818299 80818418 120 - 0.762 0.818 0.222
ENSG00000075223 E020 2.7365714 0.005771826 3.843555e-01 5.285142e-01 7 80827425 80827429 5 - 0.456 0.622 0.777
ENSG00000075223 E021 2.4463277 0.006451638 8.160179e-01 8.835604e-01 7 80827430 80827448 19 - 0.456 0.414 -0.223
ENSG00000075223 E022 2.5757065 0.046057205 7.307694e-01 8.226008e-01 7 80827449 80827487 39 - 0.456 0.530 0.361
ENSG00000075223 E023 7.5225370 0.002686690 4.543018e-01 5.949454e-01 7 80828585 80828745 161 - 0.800 0.912 0.433
ENSG00000075223 E024 0.1515154 0.041289963 1.000000e+00   7 80847260 80847345 86 - 0.054 0.001 -5.988
ENSG00000075223 E025 0.8438776 0.253636997 5.939221e-02 1.256006e-01 7 80905829 80905916 88 - 0.102 0.533 3.178
ENSG00000075223 E026 0.0000000       7 80915689 80915733 45 -      
ENSG00000075223 E027 8.1953088 0.002344781 9.512431e-01 9.735103e-01 7 80916679 80916819 141 - 0.852 0.868 0.061
ENSG00000075223 E028 0.3332198 0.028947228 1.976801e-01   7 80918301 80918429 129 - 0.054 0.255 2.585
ENSG00000075223 E029 6.6976651 0.002486351 4.235714e-03 1.380168e-02 7 80918828 80919183 356 - 0.835 0.255 -2.875
ENSG00000075223 E030 0.0000000       7 80922265 80922359 95 -