ENSG00000074935

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000368657 ENSG00000074935 HEK293_OSMI2_2hA HEK293_TMG_2hB TUBE1 protein_coding retained_intron 18.71766 10.34162 25.08782 0.6722836 0.7286205 1.277705 3.802153 1.3625541 5.440354 0.69475138 0.45168333 1.98948692 0.20300417 0.12630000 0.21613333 0.08983333 0.6813412661 0.0003313791 FALSE TRUE
ENST00000368662 ENSG00000074935 HEK293_OSMI2_2hA HEK293_TMG_2hB TUBE1 protein_coding protein_coding 18.71766 10.34162 25.08782 0.6722836 0.7286205 1.277705 4.350367 3.7965323 5.000031 0.75428278 0.52760307 0.39634209 0.25515833 0.36140000 0.19996667 -0.16143333 0.0439322995 0.0003313791 FALSE TRUE
ENST00000441191 ENSG00000074935 HEK293_OSMI2_2hA HEK293_TMG_2hB TUBE1 protein_coding processed_transcript 18.71766 10.34162 25.08782 0.6722836 0.7286205 1.277705 1.830671 2.3636430 2.403721 1.20816274 0.34645247 0.02415587 0.11868750 0.24383333 0.09520000 -0.14863333 0.9017537155 0.0003313791 FALSE FALSE
ENST00000604689 ENSG00000074935 HEK293_OSMI2_2hA HEK293_TMG_2hB TUBE1 protein_coding retained_intron 18.71766 10.34162 25.08782 0.6722836 0.7286205 1.277705 1.268794 0.1831988 1.531299 0.05072554 0.07642713 2.99598887 0.05767917 0.01730000 0.06126667 0.04396667 0.0005051309 0.0003313791 FALSE FALSE
ENST00000604743 ENSG00000074935 HEK293_OSMI2_2hA HEK293_TMG_2hB TUBE1 protein_coding nonsense_mediated_decay 18.71766 10.34162 25.08782 0.6722836 0.7286205 1.277705 0.253200 0.7640534 0.000000 0.52142080 0.00000000 -6.27436112 0.02088750 0.06830000 0.00000000 -0.06830000 0.0708601570 0.0003313791 FALSE FALSE
ENST00000605457 ENSG00000074935 HEK293_OSMI2_2hA HEK293_TMG_2hB TUBE1 protein_coding nonsense_mediated_decay 18.71766 10.34162 25.08782 0.6722836 0.7286205 1.277705 1.779913 0.9191634 2.747882 0.46027713 0.03627918 1.56955641 0.09344167 0.08803333 0.10980000 0.02176667 0.7956735352 0.0003313791 FALSE TRUE
MSTRG.28819.2 ENSG00000074935 HEK293_OSMI2_2hA HEK293_TMG_2hB TUBE1 protein_coding   18.71766 10.34162 25.08782 0.6722836 0.7286205 1.277705 3.800482 0.1800551 5.548607 0.18005515 0.71592147 4.87023346 0.15711667 0.01893333 0.22220000 0.20326667 0.0264280690 0.0003313791 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000074935 E001 34.374342 0.0086098233 1.767347e-02 4.643357e-02 6 112070663 112071111 449 - 1.466 1.642 0.603
ENSG00000074935 E002 32.594207 0.0008262671 2.109497e-04 1.010718e-03 6 112071112 112071264 153 - 1.417 1.641 0.766
ENSG00000074935 E003 44.764444 0.0028363279 9.502799e-02 1.829159e-01 6 112071265 112071328 64 - 1.615 1.707 0.313
ENSG00000074935 E004 110.913880 0.0002904663 2.157768e-02 5.480468e-02 6 112071329 112071570 242 - 2.071 1.990 -0.271
ENSG00000074935 E005 99.974524 0.0003196587 8.558466e-06 5.776393e-05 6 112071902 112072076 175 - 2.053 1.885 -0.565
ENSG00000074935 E006 35.639238 0.0008711061 1.219762e-02 3.400541e-02 6 112072758 112072781 24 - 1.607 1.448 -0.547
ENSG00000074935 E007 63.372606 0.0004678788 1.414733e-02 3.855969e-02 6 112072782 112072898 117 - 1.844 1.730 -0.387
ENSG00000074935 E008 67.413883 0.0045052415 4.394578e-03 1.424898e-02 6 112074710 112074850 141 - 1.882 1.727 -0.523
ENSG00000074935 E009 2.677363 0.0733052644 4.675861e-01 6.070959e-01 6 112074851 112075084 234 - 0.609 0.448 -0.762
ENSG00000074935 E010 9.410408 0.0026809560 1.319200e-01 2.367885e-01 6 112075085 112075936 852 - 1.064 0.890 -0.646
ENSG00000074935 E011 43.953317 0.0006625589 1.943933e-03 7.052456e-03 6 112075937 112075998 62 - 1.706 1.530 -0.601
ENSG00000074935 E012 63.110928 0.0025745834 6.493423e-04 2.713667e-03 6 112075999 112076112 114 - 1.862 1.682 -0.606
ENSG00000074935 E013 8.500374 0.0021438531 2.199231e-02 5.566004e-02 6 112076113 112076171 59 - 1.055 0.775 -1.061
ENSG00000074935 E014 21.484232 0.0017278474 3.817400e-01 5.260480e-01 6 112076172 112076321 150 - 1.377 1.308 -0.238
ENSG00000074935 E015 87.265490 0.0004485448 6.758029e-01 7.809541e-01 6 112076322 112076434 113 - 1.933 1.946 0.046
ENSG00000074935 E016 98.601706 0.0026463486 1.182609e-03 4.582472e-03 6 112076435 112076509 75 - 1.935 2.063 0.428
ENSG00000074935 E017 53.439291 0.0005510406 1.758050e-19 1.060354e-17 6 112076510 112078694 2185 - 1.866 1.337 -1.805
ENSG00000074935 E018 10.075084 0.0219116473 1.332805e-07 1.322107e-06 6 112079400 112079632 233 - 1.212 0.370 -3.505
ENSG00000074935 E019 137.792649 0.0003220735 2.431988e-04 1.146815e-03 6 112079633 112079754 122 - 2.092 2.199 0.359
ENSG00000074935 E020 145.941136 0.0002626112 1.223795e-03 4.721110e-03 6 112081092 112081203 112 - 2.123 2.215 0.305
ENSG00000074935 E021 75.493625 0.0003858173 3.092048e-01 4.514710e-01 6 112081204 112081207 4 - 1.864 1.904 0.134
ENSG00000074935 E022 1.838303 0.0325694521 1.995570e-01 3.256678e-01 6 112083916 112084188 273 - 0.525 0.279 -1.382
ENSG00000074935 E023 91.055440 0.0003027495 1.390351e-02 3.800877e-02 6 112084189 112084246 58 - 1.923 2.012 0.298
ENSG00000074935 E024 2.676422 0.0316339266 4.331346e-04 1.901823e-03 6 112085334 112085654 321 - 0.719 0.001 -11.839
ENSG00000074935 E025 1.435051 0.0098261080 9.602940e-01 9.789945e-01 6 112085655 112085674 20 - 0.382 0.371 -0.061
ENSG00000074935 E026 2.434697 0.0062704965 6.398534e-01 7.531753e-01 6 112085675 112085743 69 - 0.494 0.569 0.354
ENSG00000074935 E027 93.707976 0.0002950656 1.266992e-03 4.867123e-03 6 112086556 112086608 53 - 1.923 2.038 0.385
ENSG00000074935 E028 2.986347 0.0086708155 1.871451e-03 6.827149e-03 6 112086814 112087232 419 - 0.738 0.162 -3.309
ENSG00000074935 E029 83.914594 0.0003725675 5.562395e-03 1.745332e-02 6 112087233 112087306 74 - 1.879 1.984 0.352
ENSG00000074935 E030 60.721486 0.0004173955 4.581000e-03 1.476700e-02 6 112087410 112087529 120 - 1.730 1.856 0.425