ENSG00000074621

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000261892 ENSG00000074621 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC24A1 protein_coding protein_coding 4.652763 4.456464 4.805637 0.5242092 0.1692725 0.1085936 0.4099190 0.5031215 0.2476973 0.27165678 0.24769734 -0.9936229 0.08968750 0.11813333 0.04850000 -0.06963333 0.58852714 0.007800441 FALSE TRUE
ENST00000339868 ENSG00000074621 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC24A1 protein_coding protein_coding 4.652763 4.456464 4.805637 0.5242092 0.1692725 0.1085936 0.2205608 0.4988273 0.1833631 0.26205027 0.09369822 -1.3958639 0.04982917 0.11960000 0.03926667 -0.08033333 0.79749232 0.007800441 FALSE TRUE
ENST00000425561 ENSG00000074621 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC24A1 protein_coding processed_transcript 4.652763 4.456464 4.805637 0.5242092 0.1692725 0.1085936 0.3516213 0.5177697 0.5785743 0.26083134 0.29881000 0.1573159 0.06777500 0.10406667 0.11650000 0.01243333 1.00000000 0.007800441   FALSE
ENST00000434116 ENSG00000074621 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC24A1 protein_coding retained_intron 4.652763 4.456464 4.805637 0.5242092 0.1692725 0.1085936 1.1420455 0.8233983 1.4695737 0.08413164 0.06720598 0.8281035 0.24666667 0.18630000 0.30700000 0.12070000 0.04008473 0.007800441 FALSE TRUE
ENST00000505666 ENSG00000074621 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC24A1 protein_coding protein_coding 4.652763 4.456464 4.805637 0.5242092 0.1692725 0.1085936 0.3987226 0.0000000 0.1821751 0.00000000 0.18217507 4.2643493 0.08592083 0.00000000 0.04030000 0.04030000 0.83829923 0.007800441 FALSE FALSE
ENST00000544319 ENSG00000074621 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC24A1 protein_coding protein_coding 4.652763 4.456464 4.805637 0.5242092 0.1692725 0.1085936 0.4635403 0.1869609 0.5043922 0.06618695 0.01717607 1.3849596 0.09909583 0.03926667 0.10523333 0.06596667 0.02748888 0.007800441 FALSE TRUE
MSTRG.11066.15 ENSG00000074621 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC24A1 protein_coding   4.652763 4.456464 4.805637 0.5242092 0.1692725 0.1085936 0.7875822 1.0483020 0.9034605 0.53243947 0.12428093 -0.2123371 0.16712500 0.21030000 0.18886667 -0.02143333 0.94549447 0.007800441 FALSE TRUE
MSTRG.11066.7 ENSG00000074621 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC24A1 protein_coding   4.652763 4.456464 4.805637 0.5242092 0.1692725 0.1085936 0.1756777 0.3810749 0.0000000 0.38107485 0.00000000 -5.2893729 0.04143750 0.11176667 0.00000000 -0.11176667 0.79641217 0.007800441 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000074621 E001 2.3959726 0.0075366495 6.451603e-01 7.573271e-01 15 65611366 65611377 12 + 0.477 0.550 0.352
ENSG00000074621 E002 8.7168856 0.0514603083 8.180623e-01 8.849883e-01 15 65611378 65611404 27 + 0.953 0.991 0.140
ENSG00000074621 E003 29.0333324 0.0126756007 5.261924e-02 1.138159e-01 15 65611405 65611767 363 + 1.547 1.390 -0.541
ENSG00000074621 E004 8.4596458 0.0186898983 1.939923e-01 3.188110e-01 15 65611768 65611811 44 + 1.054 0.887 -0.623
ENSG00000074621 E005 9.6516798 0.0573473986 3.665317e-01 5.108727e-01 15 65611812 65611862 51 + 1.091 0.945 -0.539
ENSG00000074621 E006 7.3642333 0.0820761227 3.082957e-01 4.505639e-01 15 65611863 65611902 40 + 1.014 0.813 -0.765
ENSG00000074621 E007 33.2711836 0.0076180778 4.921065e-04 2.130149e-03 15 65611903 65612306 404 + 1.650 1.398 -0.863
ENSG00000074621 E008 19.0434074 0.0010240405 3.193873e-03 1.083752e-02 15 65612307 65612429 123 + 1.409 1.176 -0.814
ENSG00000074621 E009 12.8804953 0.0014211732 1.355672e-01 2.419049e-01 15 65612430 65612483 54 + 1.212 1.073 -0.501
ENSG00000074621 E010 15.9764340 0.0011753007 1.402252e-01 2.483267e-01 15 65612484 65612579 96 + 1.293 1.167 -0.446
ENSG00000074621 E011 9.5809286 0.0018373693 2.998357e-01 4.414179e-01 15 65612580 65612613 34 + 1.078 0.967 -0.408
ENSG00000074621 E012 15.4018100 0.0011620384 5.655880e-02 1.206931e-01 15 65618880 65618980 101 + 1.293 1.128 -0.586
ENSG00000074621 E013 0.0000000       15 65621932 65621937 6 +      
ENSG00000074621 E014 0.0000000       15 65621938 65622008 71 +      
ENSG00000074621 E015 0.0000000       15 65622009 65622092 84 +      
ENSG00000074621 E016 10.8615730 0.0020472783 1.465157e-02 3.970953e-02 15 65623955 65623998 44 + 1.185 0.937 -0.904
ENSG00000074621 E017 14.5301502 0.0014121877 1.897268e-03 6.905773e-03 15 65623999 65624080 82 + 1.315 1.036 -0.992
ENSG00000074621 E018 29.0506925 0.0007303036 4.256110e-03 1.385836e-02 15 65624081 65624342 262 + 1.564 1.379 -0.635
ENSG00000074621 E019 113.2149549 0.0003078610 1.145615e-01 2.121994e-01 15 65624343 65625816 1474 + 2.081 2.028 -0.177
ENSG00000074621 E020 23.3960297 0.0011122750 9.682176e-01 9.841449e-01 15 65625817 65625970 154 + 1.385 1.384 -0.004
ENSG00000074621 E021 3.6690387 0.0099179988 9.391986e-01 9.658157e-01 15 65638128 65638138 11 + 0.668 0.654 -0.062
ENSG00000074621 E022 4.9266488 0.0033858539 6.466663e-01 7.585038e-01 15 65638139 65638181 43 + 0.726 0.785 0.237
ENSG00000074621 E023 19.6282392 0.0010069958 7.274964e-01 8.201752e-01 15 65639595 65639703 109 + 1.300 1.323 0.080
ENSG00000074621 E024 0.4417471 0.7516533288 3.034982e-01 4.454184e-01 15 65642905 65643006 102 + 0.300 0.000 -11.500
ENSG00000074621 E025 0.5514428 0.0196740114 8.893391e-02 1.734931e-01 15 65643928 65644426 499 + 0.000 0.291 11.934
ENSG00000074621 E026 20.0670641 0.0009522074 6.603988e-01 7.690641e-01 15 65644427 65644513 87 + 1.300 1.330 0.103
ENSG00000074621 E027 21.5356297 0.0010731487 7.575896e-01 8.423556e-01 15 65645612 65645703 92 + 1.342 1.361 0.066
ENSG00000074621 E028 50.9419631 0.0011944566 9.728065e-01 9.870299e-01 15 65650382 65650616 235 + 1.712 1.708 -0.016
ENSG00000074621 E029 49.9933746 0.0005350115 7.364205e-02 1.493616e-01 15 65650617 65650922 306 + 1.652 1.736 0.284
ENSG00000074621 E030 15.2743390 0.0087869291 6.993031e-01 7.990980e-01 15 65650923 65650942 20 + 1.194 1.227 0.116
ENSG00000074621 E031 18.7389267 0.0010575288 1.557403e-01 2.694207e-01 15 65651670 65651759 90 + 1.221 1.330 0.382
ENSG00000074621 E032 7.0612595 0.0025343889 2.139891e-01 3.432417e-01 15 65651760 65652641 882 + 0.823 0.967 0.548
ENSG00000074621 E033 24.7346847 0.0008040588 7.477873e-03 2.245578e-02 15 65652642 65652738 97 + 1.293 1.477 0.636
ENSG00000074621 E034 23.2472899 0.0008856285 2.249078e-01 3.563176e-01 15 65652739 65652808 70 + 1.335 1.417 0.285
ENSG00000074621 E035 2.8734347 0.0068639319 7.598438e-01 8.441024e-01 15 65652809 65652929 121 + 0.601 0.550 -0.232
ENSG00000074621 E036 45.8753155 0.0019656224 8.364178e-03 2.469906e-02 15 65653830 65654729 900 + 1.587 1.726 0.472
ENSG00000074621 E037 93.2886398 0.0007342675 3.749636e-14 1.132946e-12 15 65654730 65656260 1531 + 1.792 2.072 0.944
ENSG00000074621 E038 4.0149323 0.0038919132 4.965639e-01 6.331924e-01 15 65660279 65660338 60 + 0.753 0.653 -0.412
ENSG00000074621 E039 4.6320515 0.0036335314 7.786458e-01 8.575737e-01 15 65660693 65661229 537 + 0.726 0.762 0.143