ENSG00000074527

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000343702 ENSG00000074527 HEK293_OSMI2_2hA HEK293_TMG_2hB NTN4 protein_coding protein_coding 0.5550021 0.4883853 0.8942982 0.02605843 0.1024233 0.859537 0.23877746 0.27433206 0.44505788 0.02662336 0.11006902 0.6784732 0.47083333 0.56413333 0.53826667 -0.02586667 1.000000e+00 1.701511e-06 FALSE TRUE
ENST00000344911 ENSG00000074527 HEK293_OSMI2_2hA HEK293_TMG_2hB NTN4 protein_coding protein_coding 0.5550021 0.4883853 0.8942982 0.02605843 0.1024233 0.859537 0.13689337 0.00000000 0.21262007 0.00000000 0.21262007 4.4765118 0.20484583 0.00000000 0.19596667 0.19596667 9.009613e-01 1.701511e-06 FALSE TRUE
ENST00000553059 ENSG00000074527 HEK293_OSMI2_2hA HEK293_TMG_2hB NTN4 protein_coding protein_coding 0.5550021 0.4883853 0.8942982 0.02605843 0.1024233 0.859537 0.02442584 0.00000000 0.19540669 0.00000000 0.10871358 4.3604113 0.02620417 0.00000000 0.20963333 0.20963333 2.988886e-01 1.701511e-06 FALSE TRUE
ENST00000674345 ENSG00000074527 HEK293_OSMI2_2hA HEK293_TMG_2hB NTN4 protein_coding protein_coding 0.5550021 0.4883853 0.8942982 0.02605843 0.1024233 0.859537 0.02915759 0.01173658 0.04121359 0.01173658 0.04121359 1.2364016 0.05522500 0.02236667 0.05613333 0.03376667 1.000000e+00 1.701511e-06 FALSE TRUE
ENST00000674466 ENSG00000074527 HEK293_OSMI2_2hA HEK293_TMG_2hB NTN4 protein_coding retained_intron 0.5550021 0.4883853 0.8942982 0.02605843 0.1024233 0.859537 0.08653268 0.20231668 0.00000000 0.03704956 0.00000000 -4.4081458 0.18879583 0.41346667 0.00000000 -0.41346667 1.701511e-06 1.701511e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000074527 E001 16.2869782 0.001370497 0.0051550001 0.016351052 12 95657807 95658987 1181 - 1.122 1.347 0.795
ENSG00000074527 E002 3.4215692 0.005007136 0.0404853607 0.091910390 12 95658988 95659085 98 - 0.498 0.808 1.338
ENSG00000074527 E003 3.5450632 0.004483918 0.3198488376 0.462922619 12 95659086 95659222 137 - 0.687 0.540 -0.647
ENSG00000074527 E004 0.6934950 0.020841102 0.4311355172 0.573731499 12 95663391 95663633 243 - 0.155 0.299 1.204
ENSG00000074527 E005 4.4392922 0.004031977 0.4118563258 0.555396984 12 95665810 95665980 171 - 0.758 0.649 -0.452
ENSG00000074527 E006 3.5858601 0.024301128 0.7092105664 0.806648621 12 95670078 95670146 69 - 0.625 0.693 0.289
ENSG00000074527 E007 5.1358825 0.003580552 0.3868738650 0.531044402 12 95682707 95682822 116 - 0.804 0.694 -0.443
ENSG00000074527 E008 8.6432416 0.033150602 0.1873215149 0.310370906 12 95683498 95683711 214 - 1.010 0.843 -0.631
ENSG00000074527 E009 7.2606449 0.002785447 0.0252240829 0.062335336 12 95710441 95710629 189 - 0.972 0.694 -1.084
ENSG00000074527 E010 2.6417617 0.026191765 0.5247817828 0.658225357 12 95713212 95713296 85 - 0.579 0.473 -0.502
ENSG00000074527 E011 1.7444242 0.008820633 0.7928808593 0.867552458 12 95713297 95713338 42 - 0.434 0.395 -0.214
ENSG00000074527 E012 3.7781642 0.004382309 0.0834280633 0.164931245 12 95737866 95738088 223 - 0.741 0.473 -1.192
ENSG00000074527 E013 2.8580247 0.006096025 0.1282303569 0.231682687 12 95738089 95738144 56 - 0.647 0.394 -1.215
ENSG00000074527 E014 0.0000000       12 95739896 95739995 100 -      
ENSG00000074527 E015 1.1780267 0.012112449 0.0001003186 0.000523339 12 95786783 95786938 156 - 0.000 0.648 13.082
ENSG00000074527 E016 10.6760076 0.001850630 0.3661780734 0.510522563 12 95786939 95787468 530 - 1.010 1.109 0.359
ENSG00000074527 E017 0.1515154 0.042000975 1.0000000000   12 95789786 95789948 163 - 0.084 0.000 -8.256
ENSG00000074527 E018 0.3332198 0.027412876 0.5553491119   12 95790251 95790254 4 - 0.084 0.174 1.201
ENSG00000074527 E019 1.1103767 0.013786620 0.8941742763 0.936348951 12 95790255 95790309 55 - 0.317 0.298 -0.121
ENSG00000074527 E020 1.1395084 0.012188668 0.8922694143 0.935178269 12 95790310 95790330 21 - 0.317 0.298 -0.119
ENSG00000074527 E021 3.3045752 0.054435812 0.5212012855 0.655083096 12 95790331 95790727 397 - 0.646 0.542 -0.467
ENSG00000074527 E022 1.3619978 0.010709308 0.2638166099 0.401730801 12 95790728 95790764 37 - 0.269 0.474 1.202
ENSG00000074527 E023 0.0000000       12 95791112 95791152 41 -      
ENSG00000074527 E024 0.0000000       12 95791153 95791189 37 -