ENSG00000074356

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000389005 ENSG00000074356 HEK293_OSMI2_2hA HEK293_TMG_2hB NCBP3 protein_coding protein_coding 27.19922 17.29055 37.03746 0.6403288 0.601305 1.098557 2.295876 2.4804325 1.711250 0.25313300 0.04567245 -0.5329399 0.10685000 0.14496667 0.04626667 -0.098700000 1.227063e-06 1.98745e-15 FALSE TRUE
ENST00000574379 ENSG00000074356 HEK293_OSMI2_2hA HEK293_TMG_2hB NCBP3 protein_coding retained_intron 27.19922 17.29055 37.03746 0.6403288 0.601305 1.098557 2.447323 0.5318988 4.590393 0.09257495 0.35159455 3.0856618 0.08264583 0.03093333 0.12433333 0.093400000 1.043851e-06 1.98745e-15 FALSE FALSE
ENST00000574911 ENSG00000074356 HEK293_OSMI2_2hA HEK293_TMG_2hB NCBP3 protein_coding nonsense_mediated_decay 27.19922 17.29055 37.03746 0.6403288 0.601305 1.098557 4.481561 4.7708785 3.486453 0.34363028 0.23853927 -0.4513836 0.17552500 0.27550000 0.09396667 -0.181533333 1.987450e-15 1.98745e-15 FALSE TRUE
ENST00000575815 ENSG00000074356 HEK293_OSMI2_2hA HEK293_TMG_2hB NCBP3 protein_coding retained_intron 27.19922 17.29055 37.03746 0.6403288 0.601305 1.098557 1.504064 0.2474289 3.170760 0.01643398 0.18430210 3.6271258 0.04957500 0.01440000 0.08583333 0.071433333 1.067311e-11 1.98745e-15 FALSE TRUE
ENST00000577169 ENSG00000074356 HEK293_OSMI2_2hA HEK293_TMG_2hB NCBP3 protein_coding processed_transcript 27.19922 17.29055 37.03746 0.6403288 0.601305 1.098557 2.296617 0.5966859 4.311220 0.13385571 0.34773350 2.8324170 0.07882500 0.03443333 0.11670000 0.082266667 7.603373e-05 1.98745e-15 FALSE TRUE
MSTRG.13440.1 ENSG00000074356 HEK293_OSMI2_2hA HEK293_TMG_2hB NCBP3 protein_coding   27.19922 17.29055 37.03746 0.6403288 0.601305 1.098557 11.995132 7.2525562 16.867406 0.28264181 1.21783112 1.2165438 0.42250833 0.41980000 0.45460000 0.034800000 6.941194e-01 1.98745e-15 TRUE TRUE
MSTRG.13440.2 ENSG00000074356 HEK293_OSMI2_2hA HEK293_TMG_2hB NCBP3 protein_coding   27.19922 17.29055 37.03746 0.6403288 0.601305 1.098557 2.048003 1.4106671 2.899978 0.42710458 0.00905753 1.0344398 0.07736667 0.07990000 0.07833333 -0.001566667 9.936400e-01 1.98745e-15 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000074356 E001 0.00000       17 3802158 3802164 7 -      
ENSG00000074356 E002 40.96585 0.0178893086 3.272563e-04 1.489685e-03 17 3802165 3802481 317 - 1.449 1.775 1.109
ENSG00000074356 E003 48.87074 0.0055019633 3.630780e-13 9.540087e-12 17 3802482 3803181 700 - 1.419 1.895 1.620
ENSG00000074356 E004 74.02570 0.0037238020 1.226855e-06 9.997165e-06 17 3803182 3805048 1867 - 1.734 1.995 0.881
ENSG00000074356 E005 869.33138 0.0014623379 2.274966e-13 6.149031e-12 17 3805049 3811522 6474 - 2.862 3.018 0.521
ENSG00000074356 E006 18.73021 0.0078895628 4.749823e-03 1.523241e-02 17 3811523 3811527 5 - 1.387 1.150 -0.835
ENSG00000074356 E007 319.64728 0.0010492430 2.015855e-09 2.818849e-08 17 3811528 3812493 966 - 2.424 2.590 0.551
ENSG00000074356 E008 121.46621 0.0003306257 2.740440e-05 1.645384e-04 17 3812494 3812539 46 - 2.013 2.169 0.522
ENSG00000074356 E009 157.54037 0.0002108926 5.332099e-07 4.691390e-06 17 3812540 3812654 115 - 2.121 2.282 0.540
ENSG00000074356 E010 154.34624 0.0002343874 4.404599e-08 4.801693e-07 17 3812655 3813116 462 - 2.103 2.279 0.590
ENSG00000074356 E011 197.41156 0.0012792951 1.883967e-01 3.117107e-01 17 3813117 3813279 163 - 2.270 2.328 0.196
ENSG00000074356 E012 206.26916 0.0013642338 8.031740e-01 8.747128e-01 17 3814322 3814483 162 - 2.309 2.320 0.036
ENSG00000074356 E013 186.27425 0.0002415691 1.338975e-02 3.682226e-02 17 3816116 3816270 155 - 2.290 2.244 -0.152
ENSG00000074356 E014 280.23419 0.0004301663 1.133009e-03 4.414650e-03 17 3818263 3818572 310 - 2.473 2.420 -0.177
ENSG00000074356 E015 173.65975 0.0017069263 1.818995e-05 1.139259e-04 17 3821249 3821352 104 - 2.292 2.171 -0.405
ENSG00000074356 E016 189.29308 0.0002047119 1.984830e-07 1.902840e-06 17 3821953 3822052 100 - 2.326 2.209 -0.390
ENSG00000074356 E017 34.84055 0.0010652682 1.086675e-18 5.977565e-17 17 3822053 3822456 404 - 1.727 1.126 -2.083
ENSG00000074356 E018 36.25850 0.0032135120 6.117216e-16 2.376719e-14 17 3822504 3822898 395 - 1.740 1.174 -1.952
ENSG00000074356 E019 20.80562 0.0060912395 4.219405e-13 1.098466e-11 17 3823793 3824059 267 - 1.524 0.819 -2.536
ENSG00000074356 E020 13.21107 0.0012999285 4.713008e-06 3.374779e-05 17 3824060 3824092 33 - 1.292 0.844 -1.637
ENSG00000074356 E021 35.51240 0.0007131158 6.316854e-26 7.507585e-24 17 3824093 3824941 849 - 1.758 1.007 -2.620
ENSG00000074356 E022 189.76292 0.0001907333 7.784675e-10 1.178413e-08 17 3824942 3825050 109 - 2.337 2.194 -0.475
ENSG00000074356 E023 180.71238 0.0003623886 3.626201e-08 4.017581e-07 17 3825767 3825843 77 - 2.312 2.180 -0.444
ENSG00000074356 E024 175.18271 0.0002848039 6.800222e-07 5.854229e-06 17 3826087 3826215 129 - 2.293 2.175 -0.394
ENSG00000074356 E025 160.78357 0.0002842304 1.762219e-08 2.076292e-07 17 3829243 3829368 126 - 2.264 2.120 -0.482
ENSG00000074356 E026 163.71717 0.0059852112 2.838673e-01 4.240650e-01 17 3840100 3840205 106 - 2.227 2.194 -0.111
ENSG00000074356 E027 137.05002 0.0032368576 1.815174e-01 3.031430e-01 17 3843086 3843151 66 - 2.154 2.116 -0.126
ENSG00000074356 E028 140.66904 0.0051507588 4.283607e-01 5.711511e-01 17 3846041 3846251 211 - 2.157 2.137 -0.068