ENSG00000074047

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000341310 ENSG00000074047 HEK293_OSMI2_2hA HEK293_TMG_2hB GLI2 protein_coding nonsense_mediated_decay 2.431258 3.414121 2.101911 0.356524 0.04132149 -0.6971848 0.4405516 1.0621195 0.0000000 0.26751242 0.00000000 -6.7443219 0.1547708 0.3080667 0.0000000 -0.3080667 3.262536e-09 3.262536e-09 FALSE TRUE
ENST00000361492 ENSG00000074047 HEK293_OSMI2_2hA HEK293_TMG_2hB GLI2 protein_coding protein_coding 2.431258 3.414121 2.101911 0.356524 0.04132149 -0.6971848 0.9874600 0.8509119 1.3604322 0.26643570 0.13985143 0.6706934 0.4312292 0.2413333 0.6453333 0.4040000 2.413642e-03 3.262536e-09 FALSE TRUE
ENST00000452319 ENSG00000074047 HEK293_OSMI2_2hA HEK293_TMG_2hB GLI2 protein_coding protein_coding 2.431258 3.414121 2.101911 0.356524 0.04132149 -0.6971848 0.8526521 1.4300095 0.6297295 0.03057908 0.09645865 -1.1705445 0.3433750 0.4293000 0.3016333 -0.1276667 3.856554e-01 3.262536e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000074047 E001 0.0000000       2 120735623 120735681 59 +      
ENSG00000074047 E002 10.8551282 0.0724746750 1.287366e-02 3.562119e-02 2 120735868 120736285 418 + 1.236 0.897 -1.235
ENSG00000074047 E003 0.6298062 0.0194271282 5.592627e-01 6.873931e-01 2 120792283 120792408 126 + 0.145 0.256 1.019
ENSG00000074047 E004 0.2965864 0.2955247783 4.288463e-01   2 120792409 120792438 30 + 0.000 0.182 9.268
ENSG00000074047 E005 8.7555122 0.0021285437 7.353725e-03 2.213654e-02 2 120797291 120797320 30 + 1.123 0.855 -0.993
ENSG00000074047 E006 6.7816025 0.0025883442 4.302899e-02 9.659801e-02 2 120797321 120797344 24 + 1.004 0.784 -0.839
ENSG00000074047 E007 11.3918710 0.0015973366 2.122809e-02 5.406828e-02 2 120797345 120797468 124 + 1.194 0.993 -0.728
ENSG00000074047 E008 10.0119248 0.0020805813 5.798576e-01 7.046920e-01 2 120927361 120927466 106 + 1.052 1.016 -0.135
ENSG00000074047 E009 13.9128884 0.0013110301 9.043722e-01 9.430992e-01 2 120951243 120951445 203 + 1.148 1.158 0.037
ENSG00000074047 E010 0.0000000       2 120951446 120951763 318 +      
ENSG00000074047 E011 13.0148870 0.0014385959 9.088917e-01 9.461230e-01 2 120955245 120955430 186 + 1.123 1.133 0.037
ENSG00000074047 E012 21.8221095 0.0009764056 4.521239e-02 1.005819e-01 2 120968714 120968915 202 + 1.414 1.290 -0.432
ENSG00000074047 E013 24.8342735 0.0008689871 1.777284e-02 4.665001e-02 2 120970393 120970606 214 + 1.475 1.335 -0.486
ENSG00000074047 E014 20.6803251 0.0097125708 5.995447e-03 1.859834e-02 2 120971941 120972063 123 + 1.433 1.222 -0.736
ENSG00000074047 E015 4.6738818 0.0036640172 4.375594e-01 5.795184e-01 2 120974924 120974974 51 + 0.788 0.701 -0.356
ENSG00000074047 E016 22.8140271 0.0088952081 9.899185e-03 2.851495e-02 2 120974975 120975109 135 + 1.463 1.274 -0.657
ENSG00000074047 E017 25.3159398 0.0008035676 2.931987e-04 1.352034e-03 2 120978434 120978583 150 + 1.524 1.301 -0.770
ENSG00000074047 E018 24.9792423 0.0007880379 5.998294e-02 1.265771e-01 2 120982716 120982880 165 + 1.458 1.351 -0.370
ENSG00000074047 E019 31.1535063 0.0024190225 1.865227e-01 3.093802e-01 2 120984471 120984743 273 + 1.524 1.461 -0.218
ENSG00000074047 E020 26.7226506 0.0009613329 2.186543e-01 3.488022e-01 2 120986278 120986614 337 + 1.452 1.391 -0.211
ENSG00000074047 E021 46.6151705 0.0005443132 6.753936e-05 3.682476e-04 2 120988208 120989358 1151 + 1.757 1.580 -0.603
ENSG00000074047 E022 114.7249852 0.0003027829 3.021720e-01 4.440258e-01 2 120989359 120990589 1231 + 2.001 2.057 0.188
ENSG00000074047 E023 249.3410122 0.0025099320 4.862014e-20 3.099239e-18 2 120990590 120992653 2064 + 2.187 2.455 0.896