ENSG00000073849

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000169298 ENSG00000073849 HEK293_OSMI2_2hA HEK293_TMG_2hB ST6GAL1 protein_coding protein_coding 3.818053 3.754643 3.353535 0.2729066 0.07800631 -0.1625348 2.5585744 1.6911394 2.964119 0.2284135 0.05623531 0.8059612 0.6727333 0.44853333 0.8848667 0.43633333 1.696430e-08 1.69643e-08 FALSE TRUE
ENST00000442023 ENSG00000073849 HEK293_OSMI2_2hA HEK293_TMG_2hB ST6GAL1 protein_coding protein_coding 3.818053 3.754643 3.353535 0.2729066 0.07800631 -0.1625348 0.6367804 1.5350775 0.000000 0.3555478 0.00000000 -7.2715354 0.1674500 0.42136667 0.0000000 -0.42136667 6.800713e-08 1.69643e-08 FALSE TRUE
ENST00000677292 ENSG00000073849 HEK293_OSMI2_2hA HEK293_TMG_2hB ST6GAL1 protein_coding protein_coding 3.818053 3.754643 3.353535 0.2729066 0.07800631 -0.1625348 0.1338117 0.2373043 0.000000 0.1897144 0.00000000 -4.6282154 0.0337875 0.05763333 0.0000000 -0.05763333 2.176966e-01 1.69643e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000073849 E001 0.1451727 0.0437741939 4.581852e-01   3 186930325 186930501 177 + 0.123 0.000 -10.543
ENSG00000073849 E002 1.3253775 0.0098885944 5.574321e-01 6.858372e-01 3 186930502 186930525 24 + 0.297 0.418 0.716
ENSG00000073849 E003 1.8402886 0.0078620728 4.445059e-01 5.860595e-01 3 186930526 186930531 6 + 0.364 0.513 0.787
ENSG00000073849 E004 1.8402886 0.0078620728 4.445059e-01 5.860595e-01 3 186930532 186930533 2 + 0.364 0.513 0.787
ENSG00000073849 E005 13.8236801 0.0013628327 4.340956e-03 1.409756e-02 3 186930534 186930771 238 + 1.279 1.055 -0.802
ENSG00000073849 E006 5.0522468 0.0033771089 6.655703e-03 2.032960e-02 3 186930772 186930772 1 + 0.931 0.592 -1.373
ENSG00000073849 E007 5.2048064 0.0033132176 1.249103e-02 3.471355e-02 3 186930773 186930778 6 + 0.931 0.627 -1.222
ENSG00000073849 E008 8.4122775 0.0021339566 5.155008e-02 1.119082e-01 3 186930779 186930834 56 + 1.060 0.873 -0.698
ENSG00000073849 E009 0.0000000       3 186931087 186931138 52 +      
ENSG00000073849 E010 0.5503986 0.0211134900 6.132867e-01 7.320197e-01 3 186931352 186931394 43 + 0.123 0.217 0.982
ENSG00000073849 E011 0.3289534 0.0289317437 9.542476e-01   3 186942348 186942427 80 + 0.123 0.122 -0.020
ENSG00000073849 E012 1.4266421 0.0352720218 1.973444e-01 3.229128e-01 3 186948855 186948938 84 + 0.219 0.469 1.571
ENSG00000073849 E013 16.5935554 0.0012561343 4.628606e-02 1.025573e-01 3 186963785 186963926 142 + 1.308 1.173 -0.476
ENSG00000073849 E014 0.1817044 0.0394147491 5.198831e-01   3 186963927 186964364 438 + 0.000 0.122 11.439
ENSG00000073849 E015 0.0000000       3 186974957 186975090 134 +      
ENSG00000073849 E016 0.0000000       3 186986316 186986717 402 +      
ENSG00000073849 E017 0.0000000       3 186996444 186996547 104 +      
ENSG00000073849 E018 0.0000000       3 186996548 186996621 74 +      
ENSG00000073849 E019 0.0000000       3 186996622 186996673 52 +      
ENSG00000073849 E020 0.4449813 0.0215000258 5.209996e-01 6.549023e-01 3 187006142 187006551 410 + 0.219 0.121 -1.022
ENSG00000073849 E021 0.1515154 0.0429445535 4.582968e-01   3 187017584 187019407 1824 + 0.123 0.000 -12.391
ENSG00000073849 E022 0.0000000       3 187021855 187021855 1 +      
ENSG00000073849 E023 0.0000000       3 187021856 187022004 149 +      
ENSG00000073849 E024 0.0000000       3 187024614 187024858 245 +      
ENSG00000073849 E025 0.0000000       3 187025479 187025482 4 +      
ENSG00000073849 E026 0.0000000       3 187025483 187025517 35 +      
ENSG00000073849 E027 0.0000000       3 187038392 187038394 3 +      
ENSG00000073849 E028 0.0000000       3 187038395 187038491 97 +      
ENSG00000073849 E029 16.0548258 0.0041915975 1.254860e-02 3.484849e-02 3 187038742 187038873 132 + 1.322 1.134 -0.665
ENSG00000073849 E030 0.1472490 0.0432953845 4.582473e-01   3 187041917 187041966 50 + 0.123 0.000 -12.389
ENSG00000073849 E031 29.8631202 0.0008176950 2.781573e-02 6.765188e-02 3 187042654 187043015 362 + 1.545 1.439 -0.363
ENSG00000073849 E032 15.5100451 0.0012365798 2.228272e-01 3.537431e-01 3 187043016 187043107 92 + 1.256 1.182 -0.261
ENSG00000073849 E033 24.9655469 0.0009093414 1.370971e-01 2.440501e-01 3 187043108 187043310 203 + 1.450 1.379 -0.245
ENSG00000073849 E034 0.0000000       3 187043743 187043879 137 +      
ENSG00000073849 E035 19.8864549 0.0018395704 1.260037e-02 3.497022e-02 3 187051249 187051346 98 + 1.402 1.242 -0.558
ENSG00000073849 E036 0.0000000       3 187051347 187051539 193 +      
ENSG00000073849 E037 0.1817044 0.0394147491 5.198831e-01   3 187064520 187064616 97 + 0.000 0.122 11.439
ENSG00000073849 E038 0.0000000       3 187071974 187072848 875 +      
ENSG00000073849 E039 18.8702187 0.0010476012 1.188182e-01 2.182573e-01 3 187072849 187072947 99 + 1.348 1.258 -0.315
ENSG00000073849 E040 0.4847352 0.2152759445 5.829310e-01 7.071385e-01 3 187073405 187074158 754 + 0.219 0.122 -1.021
ENSG00000073849 E041 25.1787986 0.0074880670 7.591007e-01 8.435350e-01 3 187074159 187074333 175 + 1.419 1.426 0.025
ENSG00000073849 E042 294.6627436 0.0022460509 1.818641e-10 3.068816e-09 3 187075562 187078553 2992 + 2.391 2.525 0.449