ENSG00000073350

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000375227 ENSG00000073350 HEK293_OSMI2_2hA HEK293_TMG_2hB LLGL2 protein_coding protein_coding 15.59964 29.50125 5.875054 1.555869 0.2521054 -2.326138 8.350153 18.102369 2.4770464 1.0021515 0.06294567 -2.8644700 0.4877667 0.61413333 0.4229000 -0.1912333 2.399509e-04 5.035417e-07 FALSE TRUE
ENST00000577500 ENSG00000073350 HEK293_OSMI2_2hA HEK293_TMG_2hB LLGL2 protein_coding retained_intron 15.59964 29.50125 5.875054 1.555869 0.2521054 -2.326138 1.483568 1.811708 0.9881674 0.1008850 0.08661809 -0.8679382 0.1179500 0.06146667 0.1685000 0.1070333 5.035417e-07 5.035417e-07 TRUE TRUE
MSTRG.15075.4 ENSG00000073350 HEK293_OSMI2_2hA HEK293_TMG_2hB LLGL2 protein_coding   15.59964 29.50125 5.875054 1.555869 0.2521054 -2.326138 1.435508 1.656550 1.1921423 0.8382481 0.19494993 -0.4712569 0.1032542 0.05766667 0.2035667 0.1459000 4.165308e-01 5.035417e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000073350 E001 0.0000000       17 75524826 75525079 254 +      
ENSG00000073350 E002 1.3317071 0.0110343590 1.892040e-01 3.127379e-01 17 75525080 75525174 95 + 0.493 0.244 -1.488
ENSG00000073350 E003 0.9170165 0.0163897791 2.474446e-01 3.829446e-01 17 75525690 75525690 1 + 0.381 0.162 -1.636
ENSG00000073350 E004 1.1384617 0.0812556114 3.845795e-01 5.287187e-01 17 75525691 75525696 6 + 0.381 0.208 -1.199
ENSG00000073350 E005 2.9411744 0.0093707642 6.561946e-03 2.008727e-02 17 75525697 75525725 29 + 0.821 0.373 -2.051
ENSG00000073350 E006 3.8311225 0.0174319442 4.244462e-03 1.382638e-02 17 75525726 75525731 6 + 0.905 0.450 -1.953
ENSG00000073350 E007 12.7994028 0.0033496474 2.455169e-04 1.156623e-03 17 75525732 75525778 47 + 1.301 0.916 -1.393
ENSG00000073350 E008 12.8338582 0.0048649243 7.412417e-04 3.047506e-03 17 75525779 75525788 10 + 1.285 0.924 -1.307
ENSG00000073350 E009 21.0677637 0.0127609994 9.639044e-02 1.849484e-01 17 75525789 75525825 37 + 1.345 1.173 -0.603
ENSG00000073350 E010 0.0000000       17 75532493 75532566 74 +      
ENSG00000073350 E011 0.0000000       17 75533642 75533705 64 +      
ENSG00000073350 E012 0.5514428 0.0196635270 7.242888e-01 8.178425e-01 17 75538531 75538636 106 + 0.000 0.162 11.260
ENSG00000073350 E013 0.0000000       17 75542635 75543072 438 +      
ENSG00000073350 E014 0.0000000       17 75543154 75543208 55 +      
ENSG00000073350 E015 0.2965864 0.2595263094 1.000000e+00   17 75543261 75543396 136 + 0.000 0.112 9.865
ENSG00000073350 E016 59.8068526 0.0031269069 6.489392e-01 7.603225e-01 17 75543397 75543501 105 + 1.663 1.632 -0.105
ENSG00000073350 E017 0.0000000       17 75546148 75546323 176 +      
ENSG00000073350 E018 86.7963442 0.0030167742 6.234755e-02 1.305979e-01 17 75556046 75556143 98 + 1.701 1.810 0.368
ENSG00000073350 E019 0.2944980 0.3406587378 3.541433e-02   17 75557805 75558154 350 + 0.381 0.000 -13.112
ENSG00000073350 E020 90.4958397 0.0018481416 2.884209e-03 9.922926e-03 17 75558155 75558236 82 + 1.663 1.833 0.576
ENSG00000073350 E021 109.8754242 0.0003331360 8.606633e-06 5.805179e-05 17 75558512 75558627 116 + 1.701 1.924 0.753
ENSG00000073350 E022 0.1482932 0.0418516317 1.000000e+00   17 75558975 75559251 277 + 0.000 0.061 9.560
ENSG00000073350 E023 126.0150962 0.0008392224 2.753003e-08 3.122482e-07 17 75559252 75559410 159 + 1.713 1.989 0.931
ENSG00000073350 E024 1.2814694 0.0116294564 8.169843e-01 8.842278e-01 17 75562280 75563015 736 + 0.231 0.280 0.362
ENSG00000073350 E025 133.4279879 0.0002989867 5.284088e-04 2.265662e-03 17 75563016 75563178 163 + 1.849 2.000 0.506
ENSG00000073350 E026 0.3634088 0.4219331007 1.000000e+00   17 75563179 75563330 152 + 0.000 0.115 9.714
ENSG00000073350 E027 151.6124832 0.0002304745 1.470363e-03 5.540532e-03 17 75563331 75563463 133 + 1.924 2.052 0.427
ENSG00000073350 E028 1.9552723 0.0072205400 1.172646e-02 3.288007e-02 17 75563464 75563751 288 + 0.718 0.279 -2.225
ENSG00000073350 E029 80.3711052 0.0004016960 5.874307e-02 1.244709e-01 17 75563752 75563773 22 + 1.676 1.778 0.346
ENSG00000073350 E030 90.5492629 0.0003651448 2.090147e-02 5.338135e-02 17 75563774 75563806 33 + 1.707 1.827 0.405
ENSG00000073350 E031 176.4266789 0.0002226875 2.578040e-04 1.207720e-03 17 75564353 75564507 155 + 1.982 2.118 0.456
ENSG00000073350 E032 109.8380211 0.0003317015 2.564258e-07 2.406097e-06 17 75564508 75564723 216 + 1.663 1.928 0.895
ENSG00000073350 E033 33.7478884 0.0006183652 6.268658e-09 8.036124e-08 17 75567920 75568475 556 + 1.701 1.313 -1.332
ENSG00000073350 E034 57.1000594 0.0004233248 3.392623e-07 3.105238e-06 17 75568476 75568636 161 + 1.840 1.563 -0.942
ENSG00000073350 E035 37.9128875 0.0006132510 1.629216e-04 8.044464e-04 17 75568637 75568693 57 + 1.650 1.397 -0.866
ENSG00000073350 E036 38.0654616 0.0006118159 6.028435e-05 3.328385e-04 17 75568772 75568817 46 + 1.663 1.397 -0.912
ENSG00000073350 E037 30.6529053 0.0028600733 1.790577e-05 1.122946e-04 17 75568818 75568839 22 + 1.614 1.294 -1.104
ENSG00000073350 E038 2.7044289 0.0989555097 4.949696e-03 1.579133e-02 17 75568961 75568977 17 + 0.866 0.316 -2.568
ENSG00000073350 E039 62.8322673 0.0004129654 1.844650e-05 1.153802e-04 17 75568978 75569131 154 + 1.840 1.612 -0.774
ENSG00000073350 E040 48.6785418 0.0004628772 5.895922e-02 1.248529e-01 17 75569221 75569325 105 + 1.650 1.529 -0.410
ENSG00000073350 E041 71.3504332 0.0004078491 1.929698e-02 4.998025e-02 17 75569963 75570244 282 + 1.813 1.689 -0.419
ENSG00000073350 E042 20.4383871 0.0009055259 7.351565e-03 2.213215e-02 17 75570245 75570255 11 + 1.384 1.144 -0.843
ENSG00000073350 E043 56.4521175 0.0025031471 7.433291e-01 8.319832e-01 17 75570348 75570498 151 + 1.628 1.607 -0.074
ENSG00000073350 E044 69.2275661 0.0015573505 4.930239e-01 6.300934e-01 17 75570950 75571100 151 + 1.731 1.690 -0.137
ENSG00000073350 E045 5.5229910 0.1191506457 2.049997e-02 5.254576e-02 17 75571425 75571666 242 + 1.034 0.579 -1.816
ENSG00000073350 E046 65.0927557 0.0006781335 3.293008e-02 7.766579e-02 17 75571667 75571783 117 + 1.769 1.649 -0.405
ENSG00000073350 E047 79.6427053 0.0003468689 3.018486e-02 7.233210e-02 17 75571898 75572064 167 + 1.849 1.740 -0.370
ENSG00000073350 E048 93.1783233 0.0003539474 7.299990e-01 8.220511e-01 17 75573014 75573278 265 + 1.840 1.822 -0.062
ENSG00000073350 E049 53.3592426 0.0032164430 7.919487e-01 8.669181e-01 17 75573481 75573554 74 + 1.599 1.581 -0.061
ENSG00000073350 E050 38.4519825 0.0024135106 3.487607e-01 4.929949e-01 17 75573555 75573576 22 + 1.505 1.433 -0.245
ENSG00000073350 E051 48.6643008 0.0028764865 6.356002e-01 7.498655e-01 17 75573577 75573631 55 + 1.575 1.542 -0.113
ENSG00000073350 E052 43.4658620 0.0009552912 6.100545e-01 7.294348e-01 17 75573952 75573980 29 + 1.532 1.495 -0.127
ENSG00000073350 E053 3.6646810 0.0217816070 5.062220e-01 6.418869e-01 17 75574111 75574153 43 + 0.655 0.533 -0.542
ENSG00000073350 E054 4.0010082 0.0041211592 6.254364e-01 7.415637e-01 17 75574154 75574212 59 + 0.655 0.569 -0.376
ENSG00000073350 E055 41.8638280 0.0091567921 6.954171e-01 7.960212e-01 17 75574213 75574260 48 + 1.454 1.491 0.128
ENSG00000073350 E056 1.5049038 0.0098741344 5.905463e-01 7.133871e-01 17 75574261 75574452 192 + 0.231 0.343 0.774
ENSG00000073350 E057 24.7991580 0.0108775364 7.955107e-01 8.694090e-01 17 75574453 75574495 43 + 1.285 1.262 -0.082
ENSG00000073350 E058 4.8931202 0.0762057214 4.416850e-01 5.833268e-01 17 75574496 75574609 114 + 0.772 0.622 -0.624
ENSG00000073350 E059 41.3236632 0.0121094427 6.366602e-01 7.506247e-01 17 75574610 75574668 59 + 1.443 1.491 0.164
ENSG00000073350 E060 36.8373450 0.0006626525 9.278585e-01 9.585875e-01 17 75574669 75574870 202 + 1.432 1.437 0.017
ENSG00000073350 E061 25.4961976 0.0007612625 9.613529e-02 1.845672e-01 17 75574871 75574873 3 + 1.136 1.297 0.572
ENSG00000073350 E062 67.4951165 0.0003965356 4.595109e-02 1.019441e-01 17 75574874 75575209 336 + 1.583 1.702 0.406