ENSG00000069956

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000261845 ENSG00000069956 HEK293_OSMI2_2hA HEK293_TMG_2hB MAPK6 protein_coding protein_coding 11.06862 1.917928 22.31869 0.1394463 0.6073552 3.533775 6.81052182 1.7388582 14.33245805 0.2017458 0.49283905 3.035807 0.65262083 0.9063 0.642000000 -0.26430000 1.024334e-01 1.01418e-16 FALSE TRUE
ENST00000680777 ENSG00000069956 HEK293_OSMI2_2hA HEK293_TMG_2hB MAPK6 protein_coding protein_coding 11.06862 1.917928 22.31869 0.1394463 0.6073552 3.533775 0.05856363 0.0000000 0.00000000 0.0000000 0.00000000 0.000000 0.06573750 0.0000 0.000000000 0.00000000   1.01418e-16 FALSE TRUE
ENST00000691380 ENSG00000069956 HEK293_OSMI2_2hA HEK293_TMG_2hB MAPK6 protein_coding protein_coding 11.06862 1.917928 22.31869 0.1394463 0.6073552 3.533775 0.07386310 0.1634807 0.03645911 0.1297833 0.01832748 -1.900742 0.03158333 0.0857 0.001566667 -0.08413333 5.932658e-02 1.01418e-16 FALSE TRUE
MSTRG.10825.5 ENSG00000069956 HEK293_OSMI2_2hA HEK293_TMG_2hB MAPK6 protein_coding   11.06862 1.917928 22.31869 0.1394463 0.6073552 3.533775 3.33873714 0.0000000 6.28423537 0.0000000 0.09643167 9.297887 0.17508750 0.0000 0.282033333 0.28203333 1.014180e-16 1.01418e-16 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000069956 E001 0.1472490 0.0442619553 6.289751e-01   15 51952106 51952283 178 + 0.045 0.000 -7.727
ENSG00000069956 E002 1.5102023 0.0094674609 1.847904e-06 1.448407e-05 15 51971822 51971906 85 + 0.085 0.893 4.968
ENSG00000069956 E003 0.1515154 0.0432996116 6.285434e-01   15 51983206 51983315 110 + 0.045 0.000 -8.979
ENSG00000069956 E004 0.0000000       15 52004180 52004207 28 +      
ENSG00000069956 E005 0.0000000       15 52004208 52004264 57 +      
ENSG00000069956 E006 0.1515154 0.0432996116 6.285434e-01   15 52004265 52004402 138 + 0.045 0.000 -8.979
ENSG00000069956 E007 0.0000000       15 52019188 52019208 21 +      
ENSG00000069956 E008 0.0000000       15 52019209 52019218 10 +      
ENSG00000069956 E009 1.5532566 0.0087196191 1.000000e+00 1.000000e+00 15 52019219 52019228 10 + 0.296 0.295 -0.007
ENSG00000069956 E010 2.8701925 0.0146228010 4.667088e-01 6.062616e-01 15 52019229 52019231 3 + 0.471 0.295 -1.006
ENSG00000069956 E011 8.2588352 0.0163164914 8.799181e-02 1.720663e-01 15 52019232 52019237 6 + 0.830 0.470 -1.562
ENSG00000069956 E012 17.9151597 0.0262519954 1.432399e-02 3.896442e-02 15 52019238 52019250 13 + 1.133 0.691 -1.688
ENSG00000069956 E013 52.1816161 0.0016528021 1.422105e-03 5.379877e-03 15 52019251 52019362 112 + 1.566 1.268 -1.029
ENSG00000069956 E014 34.2755474 0.0006019357 1.759144e-04 8.607484e-04 15 52019363 52019371 9 + 1.401 0.944 -1.632
ENSG00000069956 E015 33.6083031 0.0014861386 7.958298e-05 4.259029e-04 15 52019372 52019376 5 + 1.395 0.893 -1.805
ENSG00000069956 E016 0.8921407 0.0131259083 6.831444e-01 7.864348e-01 15 52019377 52019382 6 + 0.218 0.000 -11.871
ENSG00000069956 E017 0.7406253 0.0154098141 1.000000e+00 1.000000e+00 15 52019383 52019390 8 + 0.189 0.000 -11.582
ENSG00000069956 E018 1.3318114 0.0096314765 3.821319e-01 5.263624e-01 15 52019922 52020032 111 + 0.296 0.000 -12.481
ENSG00000069956 E019 0.2955422 0.0296341734 1.143328e-01   15 52021464 52021564 101 + 0.045 0.295 3.159
ENSG00000069956 E020 0.0000000       15 52024581 52024695 115 +      
ENSG00000069956 E021 0.0000000       15 52027639 52027873 235 +      
ENSG00000069956 E022 3.4608521 0.0395181512 8.525231e-01 9.086139e-01 15 52042754 52042833 80 + 0.517 0.469 -0.236
ENSG00000069956 E023 37.9628547 0.0006454884 6.854925e-06 4.731637e-05 15 52045830 52045845 16 + 1.447 0.893 -1.985
ENSG00000069956 E024 158.8591531 0.0242944062 5.672622e-04 2.411122e-03 15 52045846 52046374 529 + 2.043 1.731 -1.048
ENSG00000069956 E025 226.2271570 0.0002232642 1.192220e-05 7.783532e-05 15 52046375 52047015 641 + 2.186 2.010 -0.590
ENSG00000069956 E026 64.3991057 0.0006435649 3.598937e-01 5.042099e-01 15 52049993 52050137 145 + 1.638 1.569 -0.236
ENSG00000069956 E027 92.3891135 0.0003116121 6.238285e-02 1.306591e-01 15 52058633 52058797 165 + 1.796 1.679 -0.398
ENSG00000069956 E028 127.3907818 0.0007500093 4.733979e-03 1.518855e-02 15 52061299 52061500 202 + 1.938 1.781 -0.528
ENSG00000069956 E029 155.7024434 0.0003185169 8.070103e-01 8.774121e-01 15 52063902 52064180 279 + 2.007 2.014 0.021
ENSG00000069956 E030 610.5801272 0.0004098099 2.128601e-38 6.654407e-36 15 52064181 52067375 3195 + 2.568 2.774 0.686
ENSG00000069956 E031 0.1472490 0.0442619553 6.289751e-01   15 52068889 52069001 113 + 0.045 0.000 -8.971