ENSG00000069509

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000378045 ENSG00000069509 HEK293_OSMI2_2hA HEK293_TMG_2hB FUNDC1 protein_coding protein_coding 27.40843 21.4431 38.85047 2.767833 1.361018 0.8571177 22.931195 17.147600 34.488175 2.5775398 1.2669261 1.00767227 0.79722083 0.79736667 0.88763333 0.09026667 0.043173193 0.004503773 FALSE  
ENST00000483115 ENSG00000069509 HEK293_OSMI2_2hA HEK293_TMG_2hB FUNDC1 protein_coding processed_transcript 27.40843 21.4431 38.85047 2.767833 1.361018 0.8571177 2.150933 1.277041 1.305194 0.5936520 0.2465185 0.03121764 0.10300417 0.05816667 0.03366667 -0.02450000 0.840158398 0.004503773 FALSE  
MSTRG.34126.5 ENSG00000069509 HEK293_OSMI2_2hA HEK293_TMG_2hB FUNDC1 protein_coding   27.40843 21.4431 38.85047 2.767833 1.361018 0.8571177 1.941550 2.937580 2.817001 0.2091383 0.2129392 -0.06025867 0.07701667 0.13976667 0.07276667 -0.06700000 0.004503773 0.004503773 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000069509 E001 0.2903454 0.2838237241 4.258779e-01   X 44482186 44482363 178 - 0.185 0.000 -9.333
ENSG00000069509 E002 10.0690768 0.0239887051 3.391718e-05 1.990801e-04 X 44523639 44523756 118 - 0.742 1.257 1.917
ENSG00000069509 E003 9.6870317 0.0017730490 3.751569e-06 2.748351e-05 X 44523757 44523771 15 - 0.742 1.239 1.852
ENSG00000069509 E004 23.2013224 0.0019532862 2.887558e-05 1.724859e-04 X 44523772 44523871 100 - 1.229 1.536 1.064
ENSG00000069509 E005 451.9712912 0.0009492974 7.354293e-01 8.261998e-01 X 44523872 44524275 404 - 2.654 2.663 0.030
ENSG00000069509 E006 240.0918831 0.0002736256 4.252125e-01 5.681547e-01 X 44527237 44527365 129 - 2.375 2.396 0.069
ENSG00000069509 E007 210.1738426 0.0002168889 7.409468e-01 8.302536e-01 X 44538467 44538542 76 - 2.328 2.324 -0.014
ENSG00000069509 E008 260.8131387 0.0002784969 1.240238e-02 3.450363e-02 X 44541945 44542101 157 - 2.438 2.389 -0.164
ENSG00000069509 E009 8.3735521 0.0020153919 3.300043e-01 4.735913e-01 X 44542102 44542304 203 - 0.913 1.024 0.413
ENSG00000069509 E010 2.2308570 0.1743077245 5.790195e-01 7.040074e-01 X 44542392 44542399 8 - 0.562 0.418 -0.711
ENSG00000069509 E011 32.4309562 0.0086842817 1.219927e-03 4.708106e-03 X 44542400 44542804 405 - 1.397 1.633 0.809
ENSG00000069509 E012 120.3172634 0.0003274110 8.880088e-07 7.457361e-06 X 44542805 44542955 151 - 2.145 1.988 -0.524