ENSG00000069345

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000317089 ENSG00000069345 HEK293_OSMI2_2hA HEK293_TMG_2hB DNAJA2 protein_coding protein_coding 48.04807 21.21692 85.85978 2.758475 2.76933 2.016255 13.817167 10.495415 21.211409 2.47281247 1.1727767 1.014387 0.3301625 0.48230000 0.24670000 -0.2356 1.836900e-04 3.482996e-07 FALSE TRUE
ENST00000563158 ENSG00000069345 HEK293_OSMI2_2hA HEK293_TMG_2hB DNAJA2 protein_coding nonsense_mediated_decay 48.04807 21.21692 85.85978 2.758475 2.76933 2.016255 4.959031 2.397055 6.954915 0.25444289 0.7682488 1.532837 0.1196625 0.11763333 0.08163333 -0.0360 5.026118e-01 3.482996e-07 FALSE TRUE
MSTRG.12567.1 ENSG00000069345 HEK293_OSMI2_2hA HEK293_TMG_2hB DNAJA2 protein_coding   48.04807 21.21692 85.85978 2.758475 2.76933 2.016255 25.950447 6.436826 51.602398 0.09867447 2.2146986 3.001057 0.4765792 0.31230000 0.60060000 0.2883 3.482996e-07 3.482996e-07 FALSE TRUE
MSTRG.12567.3 ENSG00000069345 HEK293_OSMI2_2hA HEK293_TMG_2hB DNAJA2 protein_coding   48.04807 21.21692 85.85978 2.758475 2.76933 2.016255 2.581450 1.617996 5.155529 0.37795937 0.4556703 1.665819 0.0536625 0.07453333 0.06013333 -0.0144 5.557543e-01 3.482996e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000069345 E001 0.1472490 0.0457130750 1.000000e+00   16 46951913 46951946 34 - 0.076 0.000 -7.807
ENSG00000069345 E002 0.4427912 0.1834431776 8.085411e-01 8.783866e-01 16 46955362 46955390 29 - 0.141 0.189 0.501
ENSG00000069345 E003 70.2445282 0.0107883205 9.621456e-02 1.846988e-01 16 46955391 46955622 232 - 1.775 1.916 0.477
ENSG00000069345 E004 393.4050334 0.0031232311 2.471812e-12 5.689191e-11 16 46955623 46956621 999 - 2.479 2.711 0.772
ENSG00000069345 E005 486.0938955 0.0008829538 1.642429e-29 2.634659e-27 16 46956622 46957006 385 - 2.567 2.811 0.811
ENSG00000069345 E006 441.2351990 0.0001556210 2.820508e-02 6.842051e-02 16 46957007 46957220 214 - 2.596 2.649 0.175
ENSG00000069345 E007 1.5188264 0.0094797011 6.361615e-01 7.502514e-01 16 46958563 46959002 440 - 0.405 0.322 -0.485
ENSG00000069345 E008 313.5053666 0.0001596982 1.964620e-05 1.221140e-04 16 46959003 46959130 128 - 2.486 2.403 -0.279
ENSG00000069345 E009 307.9987487 0.0001573848 1.533124e-15 5.658402e-14 16 46959275 46959419 145 - 2.499 2.323 -0.587
ENSG00000069345 E010 1.1416099 0.1775833220 1.437648e-01 2.531374e-01 16 46959420 46959611 192 - 0.198 0.504 1.924
ENSG00000069345 E011 386.5173434 0.0001714109 4.478498e-03 1.448118e-02 16 46964611 46964732 122 - 2.566 2.521 -0.150
ENSG00000069345 E012 362.1105874 0.0001792099 3.075853e-02 7.343924e-02 16 46964733 46964807 75 - 2.535 2.503 -0.108
ENSG00000069345 E013 420.5199147 0.0001901408 5.620685e-01 6.897542e-01 16 46967513 46967646 134 - 2.589 2.590 0.003
ENSG00000069345 E014 295.1153545 0.0003770529 9.953289e-01 1.000000e+00 16 46968084 46968164 81 - 2.433 2.445 0.041
ENSG00000069345 E015 475.7902329 0.0001606207 3.384434e-05 1.987018e-04 16 46971349 46971572 224 - 2.660 2.597 -0.212
ENSG00000069345 E016 0.4847352 0.2977215815 7.387569e-01 8.285594e-01 16 46971573 46971659 87 - 0.142 0.191 0.515
ENSG00000069345 E017 0.8889184 0.0133948082 5.823189e-01 7.066856e-01 16 46971660 46971895 236 - 0.293 0.190 -0.808
ENSG00000069345 E018 286.2380325 0.0038911475 4.910099e-04 2.126228e-03 16 46971896 46971955 60 - 2.456 2.323 -0.444
ENSG00000069345 E019 15.6009203 0.0013053293 3.981258e-01 5.418761e-01 16 46971956 46972943 988 - 1.204 1.135 -0.247
ENSG00000069345 E020 328.0333666 0.0050328129 1.886987e-05 1.177251e-04 16 46973495 46973674 180 - 2.525 2.348 -0.589