ENSG00000069329

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000299138 ENSG00000069329 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS35 protein_coding protein_coding 40.40042 10.47981 71.15013 1.126477 3.050738 2.76208 2.004582 0.6930563 3.751235 0.1365542 0.24747181 2.41949423 0.05604583 0.06493333 0.05320000 -0.01173333 5.885169e-01 3.913127e-26 FALSE TRUE
ENST00000568784 ENSG00000069329 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS35 protein_coding nonsense_mediated_decay 40.40042 10.47981 71.15013 1.126477 3.050738 2.76208 1.379270 1.0965300 1.141110 0.2794888 0.06570848 0.05698374 0.06190000 0.10510000 0.01600000 -0.08910000 3.596155e-07 3.913127e-26 FALSE TRUE
ENST00000647959 ENSG00000069329 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS35 protein_coding nonsense_mediated_decay 40.40042 10.47981 71.15013 1.126477 3.050738 2.76208 3.628854 1.8048718 5.191269 0.2892654 0.49140495 1.51899599 0.11883333 0.17033333 0.07270000 -0.09763333 1.771700e-07 3.913127e-26 FALSE TRUE
MSTRG.12564.3 ENSG00000069329 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS35 protein_coding   40.40042 10.47981 71.15013 1.126477 3.050738 2.76208 8.961522 3.0596340 16.365917 0.2888194 0.71201438 2.41543711 0.24325417 0.29273333 0.23016667 -0.06256667 4.675346e-02 3.913127e-26 FALSE TRUE
MSTRG.12564.4 ENSG00000069329 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS35 protein_coding   40.40042 10.47981 71.15013 1.126477 3.050738 2.76208 4.194111 1.9481641 6.582145 0.4982944 0.67985284 1.75124638 0.11748750 0.18230000 0.09216667 -0.09013333 3.963814e-02 3.913127e-26 FALSE TRUE
MSTRG.12564.6 ENSG00000069329 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS35 protein_coding   40.40042 10.47981 71.15013 1.126477 3.050738 2.76208 15.027074 1.8048384 27.775333 0.1251684 1.04616650 3.93641054 0.31989583 0.17750000 0.39176667 0.21426667 1.061931e-04 3.913127e-26 FALSE TRUE
MSTRG.12564.8 ENSG00000069329 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS35 protein_coding   40.40042 10.47981 71.15013 1.126477 3.050738 2.76208 4.097757 0.0000000 8.281653 0.0000000 0.64377882 9.69551587 0.05777083 0.00000000 0.11603333 0.11603333 3.913127e-26 3.913127e-26 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000069329 E001 27.9963530 0.0029878785 6.680623e-07 5.762265e-06 16 46656132 46658076 1945 - 1.257 1.651 1.360
ENSG00000069329 E002 193.2991047 0.0004379702 1.438208e-15 5.324687e-14 16 46658077 46659012 936 - 2.120 2.384 0.881
ENSG00000069329 E003 94.0728991 0.0010873125 1.333166e-01 2.387277e-01 16 46659013 46659334 322 - 1.851 1.941 0.303
ENSG00000069329 E004 87.1686596 0.0003233581 1.615752e-01 2.771584e-01 16 46659335 46659676 342 - 1.821 1.906 0.286
ENSG00000069329 E005 348.3275830 0.0007153525 2.213736e-31 4.071705e-29 16 46659677 46660204 528 - 2.362 2.666 1.012
ENSG00000069329 E006 236.3024950 0.0095354445 1.708916e-04 8.389237e-04 16 46660205 46660335 131 - 2.214 2.444 0.768
ENSG00000069329 E007 471.4539213 0.0073710543 1.562173e-04 7.747963e-04 16 46660336 46660651 316 - 2.523 2.713 0.632
ENSG00000069329 E008 217.5342199 0.0002217924 2.669377e-04 1.244967e-03 16 46661718 46661826 109 - 2.205 2.333 0.426
ENSG00000069329 E009 168.4783832 0.0007682516 1.096778e-02 3.108637e-02 16 46661827 46661861 35 - 2.098 2.208 0.365
ENSG00000069329 E010 405.0943626 0.0002063334 9.178488e-02 1.779200e-01 16 46662243 46662482 240 - 2.488 2.545 0.189
ENSG00000069329 E011 359.7506215 0.0001655309 5.097125e-01 6.450080e-01 16 46662983 46663162 180 - 2.447 2.450 0.008
ENSG00000069329 E012 320.2076176 0.0001953131 4.371956e-01 5.792026e-01 16 46668930 46669052 123 - 2.397 2.395 -0.007
ENSG00000069329 E013 0.4407149 0.0213122343 4.376146e-01 5.795273e-01 16 46669053 46669214 162 - 0.107 0.245 1.435
ENSG00000069329 E014 366.2619586 0.0002116067 1.446433e-01 2.543433e-01 16 46671705 46671860 156 - 2.458 2.440 -0.060
ENSG00000069329 E015 357.6381553 0.0004699221 4.625942e-02 1.025139e-01 16 46672265 46672417 153 - 2.451 2.417 -0.115
ENSG00000069329 E016 244.9513319 0.0001973800 3.006131e-02 7.207432e-02 16 46672418 46672472 55 - 2.289 2.241 -0.160
ENSG00000069329 E017 340.4449238 0.0001366714 1.431547e-01 2.523153e-01 16 46674314 46674462 149 - 2.427 2.408 -0.062
ENSG00000069329 E018 259.1893228 0.0001919878 2.130189e-01 3.420939e-01 16 46674564 46674660 97 - 2.308 2.290 -0.061
ENSG00000069329 E019 0.0000000       16 46676505 46676577 73 -      
ENSG00000069329 E020 0.1472490 0.0449288055 1.000000e+00   16 46676578 46676582 5 - 0.057 0.000 -8.656
ENSG00000069329 E021 237.1031363 0.0002001549 4.000143e-02 9.104196e-02 16 46676583 46676692 110 - 2.274 2.228 -0.153
ENSG00000069329 E022 256.4741503 0.0030049522 7.585414e-02 1.528678e-01 16 46677315 46677398 84 - 2.309 2.250 -0.199
ENSG00000069329 E023 3.2559669 0.0051180573 9.110543e-02 1.768661e-01 16 46677399 46677686 288 - 0.595 0.245 -1.956
ENSG00000069329 E024 459.7075736 0.0005154851 5.661341e-08 6.029141e-07 16 46678943 46679156 214 - 2.572 2.453 -0.396
ENSG00000069329 E025 171.8824990 0.0016626419 1.379630e-03 5.239867e-03 16 46680671 46680674 4 - 2.147 2.026 -0.405
ENSG00000069329 E026 189.4689878 0.0010728303 4.251605e-03 1.384655e-02 16 46680675 46680686 12 - 2.186 2.092 -0.314
ENSG00000069329 E027 392.6893985 0.0004908568 2.149967e-08 2.491618e-07 16 46680687 46680853 167 - 2.506 2.372 -0.445
ENSG00000069329 E028 373.5255251 0.0001472574 7.375151e-13 1.848393e-11 16 46681377 46681500 124 - 2.489 2.319 -0.568
ENSG00000069329 E029 8.0308594 0.0439105865 2.582804e-01 3.955094e-01 16 46681501 46681520 20 - 0.887 0.681 -0.825
ENSG00000069329 E030 0.9016039 0.2280596344 1.000000e+00 1.000000e+00 16 46681521 46681755 235 - 0.231 0.242 0.089
ENSG00000069329 E031 1.2305573 0.1018721396 5.472755e-01 6.773405e-01 16 46681756 46682078 323 - 0.265 0.399 0.842
ENSG00000069329 E032 285.5365691 0.0008308678 3.169230e-08 3.550778e-07 16 46682079 46682175 97 - 2.373 2.210 -0.545
ENSG00000069329 E033 1.9785575 0.3743050992 5.970129e-01 7.188538e-01 16 46682478 46682642 165 - 0.430 0.246 -1.153
ENSG00000069329 E034 1.0822641 0.1857862164 1.000000e+00 1.000000e+00 16 46682643 46682666 24 - 0.265 0.245 -0.151
ENSG00000069329 E035 0.1817044 0.0424674865 7.314552e-02   16 46682667 46682684 18 - 0.000 0.245 13.255
ENSG00000069329 E036 1.5962448 0.1629228886 6.924409e-01 7.936886e-01 16 46682882 46683507 626 - 0.327 0.404 0.452
ENSG00000069329 E037 226.0488124 0.0001912775 1.122353e-08 1.370899e-07 16 46683508 46683606 99 - 2.273 2.097 -0.588
ENSG00000069329 E038 0.1451727 0.0434378786 1.000000e+00   16 46683607 46683607 1 - 0.057 0.000 -8.656
ENSG00000069329 E039 7.8427741 0.0021313205 3.082121e-06 2.299628e-05 16 46688314 46689130 817 - 0.679 1.247 2.141
ENSG00000069329 E040 87.4902573 0.0018583785 9.455876e-05 4.965180e-04 16 46689131 46689221 91 - 1.870 1.659 -0.713
ENSG00000069329 E041 4.5957023 0.0174404963 6.987299e-01 7.986226e-01 16 46689335 46689607 273 - 0.666 0.606 -0.260