ENSG00000068654

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000263857 ENSG00000068654 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR1A protein_coding protein_coding 23.0955 25.13507 21.21942 2.156523 0.4381337 -0.2442103 11.851572 16.816382 8.420278 0.7722125 0.5689139 -0.9970729 0.5069500 0.6745333 0.3972000 -0.2773333333 0.0001652263 0.0001652263 FALSE TRUE
ENST00000409681 ENSG00000068654 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR1A protein_coding protein_coding 23.0955 25.13507 21.21942 2.156523 0.4381337 -0.2442103 3.615306 0.775611 5.985217 0.5810432 0.2231239 2.9319249 0.1652833 0.0278000 0.2821667 0.2543666667 0.0267380292 0.0001652263 FALSE TRUE
MSTRG.18784.4 ENSG00000068654 HEK293_OSMI2_2hA HEK293_TMG_2hB POLR1A protein_coding   23.0955 25.13507 21.21942 2.156523 0.4381337 -0.2442103 6.625586 6.655970 5.554840 1.0074818 0.3908917 -0.2604746 0.2827292 0.2620333 0.2616000 -0.0004333333 1.0000000000 0.0001652263 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000068654 E001 20.7122441 0.0497761710 9.959363e-03 2.866171e-02 2 86020216 86020333 118 - 1.066 1.431 1.290
ENSG00000068654 E002 337.6365909 0.0144856655 1.689236e-10 2.868340e-09 2 86020334 86021272 939 - 2.200 2.635 1.450
ENSG00000068654 E003 241.8722464 0.0044316394 1.005074e-14 3.304135e-13 2 86021273 86021613 341 - 2.120 2.474 1.183
ENSG00000068654 E004 194.4812461 0.0087652225 1.696912e-06 1.340932e-05 2 86021614 86021942 329 - 2.077 2.365 0.965
ENSG00000068654 E005 345.7006370 0.0007407782 7.806949e-12 1.654551e-10 2 86021943 86022702 760 - 2.395 2.591 0.654
ENSG00000068654 E006 186.6344440 0.0002876028 2.118909e-05 1.306284e-04 2 86022703 86022994 292 - 2.160 2.312 0.507
ENSG00000068654 E007 865.9623639 0.0025202809 4.577800e-10 7.219267e-09 2 86022995 86025029 2035 - 2.791 2.988 0.657
ENSG00000068654 E008 782.6061754 0.0024664038 3.318471e-10 5.359999e-09 2 86025030 86026327 1298 - 2.745 2.945 0.666
ENSG00000068654 E009 925.8709283 0.0025529099 1.918588e-30 3.334594e-28 2 86026328 86027017 690 - 2.714 3.053 1.128
ENSG00000068654 E010 711.3165809 0.0040950916 5.217813e-15 1.789786e-13 2 86027018 86027523 506 - 2.634 2.928 0.980
ENSG00000068654 E011 222.7832059 0.0005805143 7.261097e-02 1.476923e-01 2 86027885 86027965 81 - 2.284 2.365 0.270
ENSG00000068654 E012 216.7879532 0.0004239346 3.071064e-02 7.334801e-02 2 86027966 86028049 84 - 2.265 2.355 0.300
ENSG00000068654 E013 255.1061862 0.0007647269 9.922257e-01 9.992426e-01 2 86028594 86028711 118 - 2.375 2.407 0.107
ENSG00000068654 E014 0.1451727 0.0426542683 3.414318e-01   2 86028712 86028799 88 - 0.140 0.000 -12.937
ENSG00000068654 E015 301.5999756 0.0002199732 7.503619e-01 8.370606e-01 2 86030196 86030396 201 - 2.452 2.478 0.085
ENSG00000068654 E016 165.7728063 0.0002764715 5.112432e-01 6.463104e-01 2 86031330 86031420 91 - 2.177 2.228 0.172
ENSG00000068654 E017 161.7064846 0.0033927768 1.141715e-02 3.215559e-02 2 86031421 86031503 83 - 2.111 2.247 0.455
ENSG00000068654 E018 90.1355200 0.0051174610 1.148971e-02 3.232795e-02 2 86031504 86031512 9 - 1.839 2.005 0.560
ENSG00000068654 E019 195.4189849 0.0028494318 2.881153e-01 4.286570e-01 2 86031513 86031635 123 - 2.235 2.306 0.237
ENSG00000068654 E020 145.3484999 0.0002462802 1.262534e-03 4.851587e-03 2 86032272 86032382 111 - 2.191 2.126 -0.216
ENSG00000068654 E021 163.5293872 0.0002215294 8.118166e-02 1.613845e-01 2 86033661 86033787 127 - 2.212 2.194 -0.059
ENSG00000068654 E022 0.0000000       2 86036978 86037070 93 -      
ENSG00000068654 E023 230.6547207 0.0001894188 4.058791e-01 5.495389e-01 2 86038700 86038857 158 - 2.343 2.356 0.041
ENSG00000068654 E024 0.4782907 0.0218687404 1.612477e-01 2.767120e-01 2 86038858 86038917 60 - 0.000 0.264 13.299
ENSG00000068654 E025 0.1482932 0.0415018408 6.827359e-01   2 86039325 86039326 2 - 0.000 0.107 11.614
ENSG00000068654 E026 105.4538516 0.0020168213 3.736452e-02 8.615518e-02 2 86039327 86039341 15 - 2.047 1.997 -0.170
ENSG00000068654 E027 196.9825905 0.0002420995 4.235637e-04 1.864726e-03 2 86039342 86039462 121 - 2.320 2.261 -0.198
ENSG00000068654 E028 1.5133995 0.1134203919 4.779871e-01 6.164411e-01 2 86039998 86040200 203 - 0.462 0.328 -0.754
ENSG00000068654 E029 200.8115005 0.0002199594 7.864893e-06 5.351365e-05 2 86040392 86040559 168 - 2.340 2.258 -0.275
ENSG00000068654 E030 235.8639917 0.0030801135 2.764596e-04 1.283907e-03 2 86041889 86042103 215 - 2.417 2.324 -0.311
ENSG00000068654 E031 242.0227435 0.0024012151 1.665496e-06 1.317754e-05 2 86042974 86043195 222 - 2.445 2.324 -0.404
ENSG00000068654 E032 185.7494780 0.0003896888 9.486533e-09 1.175214e-07 2 86044139 86044304 166 - 2.330 2.206 -0.414
ENSG00000068654 E033 126.8125322 0.0082695458 2.488020e-02 6.162618e-02 2 86045278 86045360 83 - 2.142 2.060 -0.277
ENSG00000068654 E034 170.8579675 0.0055295596 4.873692e-03 1.557810e-02 2 86045617 86045769 153 - 2.276 2.186 -0.300
ENSG00000068654 E035 152.8702814 0.0061782134 1.075611e-03 4.220002e-03 2 86047165 86047263 99 - 2.244 2.126 -0.394
ENSG00000068654 E036 177.6178939 0.0066380184 8.556644e-04 3.457608e-03 2 86048884 86049042 159 - 2.310 2.190 -0.401
ENSG00000068654 E037 124.7799965 0.0035468317 2.453743e-06 1.872299e-05 2 86049160 86049242 83 - 2.183 2.016 -0.559
ENSG00000068654 E038 0.1817044 0.0388851693 6.821211e-01   2 86052329 86052816 488 - 0.000 0.107 11.631
ENSG00000068654 E039 167.7936798 0.0061462020 7.313351e-05 3.952790e-04 2 86052817 86053000 184 - 2.302 2.152 -0.501
ENSG00000068654 E040 0.8480291 0.0145250764 4.529974e-02 1.007395e-01 2 86054138 86054139 2 - 0.000 0.379 14.077
ENSG00000068654 E041 162.1274715 0.0033503445 1.981703e-08 2.312886e-07 2 86054140 86054289 150 - 2.306 2.120 -0.621
ENSG00000068654 E042 188.3809579 0.0093683955 7.549600e-06 5.157024e-05 2 86065274 86065465 192 - 2.378 2.176 -0.673
ENSG00000068654 E043 0.2903454 0.3139547035 1.764193e-01   2 86065466 86065714 249 - 0.245 0.000 -13.105
ENSG00000068654 E044 203.5120526 0.0096637579 4.226407e-07 3.794153e-06 2 86070018 86070272 255 - 2.428 2.193 -0.784
ENSG00000068654 E045 0.1451727 0.0426542683 3.414318e-01   2 86074261 86075029 769 - 0.140 0.000 -12.937
ENSG00000068654 E046 181.8199873 0.0011841842 2.155706e-22 1.794341e-20 2 86075030 86075260 231 - 2.395 2.129 -0.888
ENSG00000068654 E047 148.8059893 0.0022536551 1.309732e-14 4.237827e-13 2 86077859 86077981 123 - 2.300 2.051 -0.832
ENSG00000068654 E048 0.1482932 0.0415018408 6.827359e-01   2 86078041 86078113 73 - 0.000 0.107 11.614
ENSG00000068654 E049 187.0869121 0.0023939859 2.712092e-20 1.790860e-18 2 86078114 86078284 171 - 2.416 2.128 -0.964
ENSG00000068654 E050 1.2533568 0.0104400365 5.139789e-01 6.487558e-01 2 86079773 86080815 1043 - 0.245 0.379 0.878
ENSG00000068654 E051 175.5629377 0.0013946745 7.339413e-29 1.120411e-26 2 86080816 86080978 163 - 2.404 2.081 -1.079
ENSG00000068654 E052 72.4033963 0.0142900956 3.368409e-05 1.978468e-04 2 86081601 86081616 16 - 1.997 1.730 -0.898
ENSG00000068654 E053 89.5741489 0.0082216157 3.461784e-08 3.851901e-07 2 86081617 86081706 90 - 2.097 1.812 -0.957
ENSG00000068654 E054 0.4439371 0.0215536322 4.009937e-02 9.121774e-02 2 86081707 86083081 1375 - 0.330 0.000 -14.611
ENSG00000068654 E055 97.9613250 0.0103593706 1.460140e-07 1.436833e-06 2 86083082 86083168 87 - 2.136 1.854 -0.947
ENSG00000068654 E056 6.7514150 0.0023611664 9.535231e-03 2.762407e-02 2 86086254 86088565 2312 - 1.036 0.759 -1.060
ENSG00000068654 E057 123.2268871 0.0048519650 7.306129e-11 1.318590e-09 2 86088566 86088669 104 - 2.228 1.961 -0.893
ENSG00000068654 E058 99.3004730 0.0113500384 4.865188e-05 2.749360e-04 2 86088785 86088870 86 - 2.110 1.892 -0.731
ENSG00000068654 E059 0.2966881 0.0293266085 1.167152e-01   2 86089634 86089821 188 - 0.245 0.000 -13.993
ENSG00000068654 E060 116.8015096 0.0064651658 4.806886e-09 6.294237e-08 2 86089822 86089929 108 - 2.200 1.939 -0.876
ENSG00000068654 E061 134.8255881 0.0100642603 2.375029e-11 4.656823e-10 2 86098611 86098760 150 - 2.302 1.955 -1.164
ENSG00000068654 E062 145.8919732 0.0115354306 2.400962e-09 3.317204e-08 2 86099968 86100172 205 - 2.323 2.005 -1.062
ENSG00000068654 E063 103.9522509 0.0161646512 2.064947e-04 9.917977e-04 2 86105700 86106155 456 - 2.131 1.910 -0.744