ENSG00000068305

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000354410 ENSG00000068305 HEK293_OSMI2_2hA HEK293_TMG_2hB MEF2A protein_coding protein_coding 9.905581 4.412427 15.24238 0.08920039 0.6235594 1.786124 1.3902481 0.6515459 2.1802254 0.07464832 0.30745282 1.727166 0.12530000 0.1471333 0.14190000 -0.005233333 9.528162e-01 6.750907e-15 FALSE TRUE
ENST00000449277 ENSG00000068305 HEK293_OSMI2_2hA HEK293_TMG_2hB MEF2A protein_coding protein_coding 9.905581 4.412427 15.24238 0.08920039 0.6235594 1.786124 1.3566574 0.7749364 1.6160412 0.38907905 0.20264194 1.050716 0.17724583 0.1740333 0.10563333 -0.068400000 9.906254e-01 6.750907e-15 FALSE TRUE
ENST00000557785 ENSG00000068305 HEK293_OSMI2_2hA HEK293_TMG_2hB MEF2A protein_coding protein_coding 9.905581 4.412427 15.24238 0.08920039 0.6235594 1.786124 2.6900852 0.0000000 5.2226625 0.00000000 0.60990291 9.031401 0.21945417 0.0000000 0.34616667 0.346166667 6.750907e-15 6.750907e-15 FALSE TRUE
ENST00000557942 ENSG00000068305 HEK293_OSMI2_2hA HEK293_TMG_2hB MEF2A protein_coding protein_coding 9.905581 4.412427 15.24238 0.08920039 0.6235594 1.786124 0.5656382 1.5504932 0.4182156 0.18116315 0.41821558 -1.865593 0.10155000 0.3526333 0.02966667 -0.322966667 2.743575e-02 6.750907e-15 FALSE TRUE
ENST00000691492 ENSG00000068305 HEK293_OSMI2_2hA HEK293_TMG_2hB MEF2A protein_coding protein_coding 9.905581 4.412427 15.24238 0.08920039 0.6235594 1.786124 0.8929468 0.2815877 0.8187203 0.23094506 0.81872027 1.506955 0.07737083 0.0648000 0.05040000 -0.014400000 6.323205e-01 6.750907e-15 FALSE TRUE
MSTRG.11647.2 ENSG00000068305 HEK293_OSMI2_2hA HEK293_TMG_2hB MEF2A protein_coding   9.905581 4.412427 15.24238 0.08920039 0.6235594 1.786124 0.3232543 0.0000000 0.9058076 0.00000000 0.14249477 6.516973 0.02065417 0.0000000 0.05973333 0.059733333 6.717506e-06 6.750907e-15 FALSE FALSE
MSTRG.11647.9 ENSG00000068305 HEK293_OSMI2_2hA HEK293_TMG_2hB MEF2A protein_coding   9.905581 4.412427 15.24238 0.08920039 0.6235594 1.786124 1.3194022 0.7015378 1.9737225 0.18282747 0.08235622 1.479198 0.12490000 0.1588333 0.12976667 -0.029066667 8.065919e-01 6.750907e-15 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000068305 E001 1.0373134 0.0117442579 6.191413e-02 1.298407e-01 15 99565413 99565416 4 + 0.376 0.000 -12.864
ENSG00000068305 E002 6.5001628 0.0024597651 1.084645e-02 3.079684e-02 15 99565417 99565669 253 + 0.918 0.567 -1.435
ENSG00000068305 E003 1.0738451 0.0115605987 3.839254e-01 5.281299e-01 15 99565700 99565713 14 + 0.339 0.187 -1.134
ENSG00000068305 E004 2.5068194 0.0060567340 7.649783e-01 8.478699e-01 15 99565714 99565927 214 + 0.527 0.499 -0.133
ENSG00000068305 E005 0.5117019 0.0267574522 1.209030e-02 3.374942e-02 15 99565928 99565928 1 + 0.000 0.417 14.444
ENSG00000068305 E006 0.6632174 0.0242578101 5.748974e-02 1.223228e-01 15 99565929 99565950 22 + 0.078 0.417 3.035
ENSG00000068305 E007 1.2115291 0.0102478601 8.305909e-01 8.937342e-01 15 99565951 99565968 18 + 0.339 0.317 -0.134
ENSG00000068305 E008 2.8366927 0.0163348069 2.102486e-01 3.388398e-01 15 99565969 99565979 11 + 0.618 0.417 -0.966
ENSG00000068305 E009 3.7578764 0.0257533647 3.646632e-01 5.090024e-01 15 99565980 99565983 4 + 0.693 0.566 -0.554
ENSG00000068305 E010 3.7578764 0.0257533647 3.646632e-01 5.090024e-01 15 99565984 99565986 3 + 0.693 0.566 -0.554
ENSG00000068305 E011 3.9030491 0.0189312825 3.042651e-01 4.462811e-01 15 99565987 99565989 3 + 0.710 0.566 -0.623
ENSG00000068305 E012 10.7522758 0.0330637153 1.914752e-01 3.155862e-01 15 99565990 99566012 23 + 1.080 0.931 -0.552
ENSG00000068305 E013 11.3360750 0.0529503818 1.082378e-01 2.029326e-01 15 99566013 99566017 5 + 1.114 0.903 -0.780
ENSG00000068305 E014 12.8129429 0.0125225352 8.935780e-02 1.741515e-01 15 99566018 99566026 9 + 1.152 0.983 -0.613
ENSG00000068305 E015 20.7061607 0.0031331770 1.320725e-01 2.369949e-01 15 99566027 99566104 78 + 1.332 1.234 -0.342
ENSG00000068305 E016 0.1515154 0.0431448843 1.000000e+00   15 99566483 99566599 117 + 0.078 0.000 -11.923
ENSG00000068305 E017 27.4702512 0.0029784007 3.260526e-02 7.701093e-02 15 99598430 99598511 82 + 1.456 1.321 -0.467
ENSG00000068305 E018 0.5869198 0.1762230400 2.688477e-01 4.073201e-01 15 99601941 99601999 59 + 0.252 0.000 -13.403
ENSG00000068305 E019 0.4502799 0.0304338386 3.527621e-01 4.970120e-01 15 99602000 99602109 110 + 0.202 0.000 -13.575
ENSG00000068305 E020 3.8125178 0.0047354129 9.619659e-01 9.800248e-01 15 99620891 99621080 190 + 0.657 0.678 0.087
ENSG00000068305 E021 71.4407431 0.0003973555 1.464265e-04 7.316384e-04 15 99632978 99633173 196 + 1.866 1.721 -0.487
ENSG00000068305 E022 83.6149589 0.0003372226 9.354784e-03 2.716944e-02 15 99645561 99645764 204 + 1.916 1.841 -0.254
ENSG00000068305 E023 0.5858876 0.0207994987 9.015459e-01 9.412744e-01 15 99645765 99645831 67 + 0.201 0.187 -0.135
ENSG00000068305 E024 0.6599951 0.0182294129 3.084521e-03 1.051487e-02 15 99657413 99657531 119 + 0.000 0.498 14.939
ENSG00000068305 E025 51.4492455 0.0004752630 2.207316e-01 3.512906e-01 15 99671323 99671454 132 + 1.696 1.665 -0.105
ENSG00000068305 E026 0.5933762 0.0217636190 2.197081e-01 3.500412e-01 15 99671455 99671519 65 + 0.252 0.000 -14.060
ENSG00000068305 E027 17.0707570 0.0011337628 1.075405e-01 2.019070e-01 15 99671520 99671657 138 + 1.258 1.143 -0.407
ENSG00000068305 E028 0.4407149 0.0215161074 8.498140e-01 9.068089e-01 15 99671658 99671669 12 + 0.144 0.187 0.450
ENSG00000068305 E029 94.1794414 0.0003446478 1.241661e-03 4.782146e-03 15 99674393 99674612 220 + 1.972 1.876 -0.320
ENSG00000068305 E030 57.6160345 0.0003939810 1.855711e-01 3.082163e-01 15 99675399 99675458 60 + 1.744 1.712 -0.108
ENSG00000068305 E031 93.8837591 0.0002866389 8.793290e-02 1.719813e-01 15 99690241 99690428 188 + 1.953 1.921 -0.109
ENSG00000068305 E032 5.9977431 0.0027710953 6.716872e-01 7.778682e-01 15 99694076 99694186 111 + 0.787 0.873 0.335
ENSG00000068305 E033 2.2402039 0.0842124326 2.843369e-02 6.888070e-02 15 99703362 99703385 24 + 0.297 0.728 2.144
ENSG00000068305 E034 3.4375550 0.0044818247 5.517938e-01 6.812184e-01 15 99706595 99706728 134 + 0.638 0.567 -0.314
ENSG00000068305 E035 73.6459111 0.0004092780 4.436348e-07 3.964725e-06 15 99706729 99706855 127 + 1.891 1.684 -0.697
ENSG00000068305 E036 59.2135048 0.0004272090 2.410415e-07 2.274699e-06 15 99710634 99710760 127 + 1.806 1.563 -0.826
ENSG00000068305 E037 100.3366285 0.0003073940 3.919277e-05 2.263981e-04 15 99712390 99712835 446 + 2.007 1.880 -0.427
ENSG00000068305 E038 489.5390817 0.0002384645 1.603786e-04 7.935448e-04 15 99712836 99715467 2632 + 2.625 2.717 0.304
ENSG00000068305 E039 239.0065164 0.0051644123 1.164565e-17 5.662819e-16 15 99715468 99717573 2106 + 2.207 2.557 1.168