ENSG00000067533

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000366932 ENSG00000067533 HEK293_OSMI2_2hA HEK293_TMG_2hB RRP15 protein_coding protein_coding 35.29824 33.85479 41.6624 0.5043329 1.191553 0.299306 5.528879 4.171008 8.288996 0.2529442 0.2988464 0.9890861 0.1521375 0.1230333 0.1988667 0.07583333 1.895378e-05 1.895378e-05 FALSE  
MSTRG.3059.2 ENSG00000067533 HEK293_OSMI2_2hA HEK293_TMG_2hB RRP15 protein_coding   35.29824 33.85479 41.6624 0.5043329 1.191553 0.299306 2.381600 0.000000 0.000000 0.0000000 0.0000000 0.0000000 0.0554125 0.0000000 0.0000000 0.00000000   1.895378e-05 FALSE  
MSTRG.3059.3 ENSG00000067533 HEK293_OSMI2_2hA HEK293_TMG_2hB RRP15 protein_coding   35.29824 33.85479 41.6624 0.5043329 1.191553 0.299306 27.157703 29.627895 33.053951 0.2556568 0.6032787 0.1578162 0.7868875 0.8753333 0.7938667 -0.08146667 3.450683e-04 1.895378e-05 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000067533 E001 116.1272372 0.0081602266 9.498240e-01 9.726137e-01 1 218285293 218285341 49 + 2.087 2.045 -0.142
ENSG00000067533 E002 237.1205477 0.0002100664 3.554362e-01 4.997677e-01 1 218285342 218285455 114 + 2.408 2.342 -0.219
ENSG00000067533 E003 493.1672452 0.0001646692 1.195482e-04 6.118120e-04 1 218302294 218302559 266 + 2.686 2.700 0.045
ENSG00000067533 E004 1.3180938 0.1501379864 8.148110e-02 1.618745e-01 1 218302560 218302671 112 + 0.534 0.129 -2.803
ENSG00000067533 E005 337.4811395 0.0005927756 1.050871e-13 2.976296e-12 1 218305028 218305125 98 + 2.469 2.578 0.365
ENSG00000067533 E006 467.6972010 0.0001366288 9.514104e-17 4.108544e-15 1 218307431 218307632 202 + 2.627 2.711 0.280
ENSG00000067533 E007 0.1451727 0.0429980234 6.146656e-01   1 218318407 218318495 89 + 0.115 0.000 -8.810
ENSG00000067533 E008 2.4495725 0.0441283603 8.040618e-01 8.753274e-01 1 218322736 218322804 69 + 0.572 0.491 -0.379
ENSG00000067533 E009 1021.2712573 0.0022729225 2.673317e-15 9.570142e-14 1 218330948 218337983 7036 + 3.093 2.913 -0.600